Fruit preservative based on comprehensive utilization of enteromorpha prolifera and preparation method of fruit preservative

A technology of antistaling agent and enteromorpha, which is applied in the field of natural fruit preservatives and preparations, can solve problems such as rot and easy damage, achieve strong antibacterial ability, improve antibacterial effect, and prolong the effect of fruit preservation

Active Publication Date: 2014-02-19
广西贺州市正丰现代农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fruit production has the characteristics of strong seasonality, regionality, and post-harvest damage, rot and deterioration, which contradicts the diversity of consumer demand for fruit and the urgency of off-season adjustments, and the problem of fruit storage and preservation has become increasingly prominent , it is of great significance to study natural, efficient, safe and non-toxic fruit preservatives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Degreasing of Enteromorpha powder: Take 100g of Enteromorpha dry powder in a round-bottomed flask, add 1L of ethanol with a concentration of 95%, put it in a water bath at 85°C to reflux twice for 2 hours, filter, and filter the residue Put it in an oven and dry it to get dark brown Enteromorpha dry powder;

[0023] 2) Deproteinization: take 50g of defatted Enteromorpha dry powder, extract it in hot water at 80°C for 4 hours, cool it, and adjust the pH to 5.5; add 8% papain, enzymolysis temperature 60°C, enzymolysis for 2 hours, deproteinized;

[0024] 3) Concentration and extraction: the filtrate was concentrated by a rotary evaporator, added 4 times the volume of absolute ethanol, and allowed to stand for 12 hours to obtain the crude polysaccharide of Enteromorpha enteromorpha;

[0025] 4) Preparation of selenized Enteromorpha polysaccharide: Accurately weigh 5g of Enteromorpha crude polysaccharide into a three-neck flask, add 500mL of 0.5% nitric acid solution, ...

Embodiment 2

[0030] 1) Degreasing of Enteromorpha powder: Take 100g of Enteromorpha dry powder in a round-bottomed flask, add 1L of ethanol with a concentration of 95%, put it in a water bath at 85°C to reflux twice for 2 hours, filter, and filter the residue Put it in an oven and dry it to get dark brown Enteromorpha dry powder;

[0031] 2) Deproteinization: take 50g of defatted Enteromorpha dry powder, extract it in hot water at 80°C for 4 hours, cool it, and adjust the pH to 5.5; add 8% papain, enzymolysis temperature 60°C, enzymolysis for 2 hours, deproteinized;

[0032]3) Concentration and extraction: the filtrate was concentrated by a rotary evaporator, added 4 times the volume of absolute ethanol, and allowed to stand for 12 hours to obtain the crude polysaccharide of Enteromorpha enteromorpha;

[0033] 4) Preparation of selenized Enteromorpha polysaccharide: Accurately weigh 5g of Enteromorpha crude polysaccharide into a three-neck flask, add 500mL of 0.5% nitric acid solution, he...

Embodiment 3

[0038] 1) Degreasing of Enteromorpha powder: Take a certain quality of Enteromorpha dry powder in a round-bottomed flask, add 10 times the amount of 95% ethanol, place it in a water bath at 85°C for reflux twice for 2 hours, filter, and filter the residue Put it in an oven and dry it to get dark brown Enteromorpha dry powder;

[0039] 2) Deproteinization: Take a certain amount of degreased Enteromorpha dry powder, extract it in hot water at 80 °C for 4 h, cool it, and adjust the pH to 5.5; add 8% papain, enzymatic hydrolysis temperature 60 °C, and enzymatic hydrolysis for 2 h , deproteinized;

[0040] 3) Concentration and extraction: the filtrate was concentrated by a rotary evaporator, added 4 times the volume of absolute ethanol, and allowed to stand for 12 hours to obtain the crude polysaccharide of Enteromorpha enteromorpha;

[0041] 4) Preparation of selenized Enteromorpha polysaccharide: Accurately weigh a certain amount of Enteromorpha crude polysaccharide and put it i...

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PUM

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Abstract

The invention discloses a natural, efficient, safe and nontoxic fruit preservative based on comprehensive utilization of marine suddenly aggregated green seaweed enteromorpha prolifera. The fruit preservative has a good preservation effect, prolongs the fruit preservation time, is prepared by mixing enteromorpha prolifera polysaccharide selenide, Chinese herbal medicine ethanol extracts and water and comprises the following components in percentage by mass: the enteromorpha prolifera polysaccharide selenide with the concentration of 100-200g / L, a clove ethanol extract with the concentration of 150-250g / L and a cinnamon ethanol extract with the concentration of 200-300g / L. The product can effectively prolong the fruit preservation time; during use, the fruit preservative can be diluted by 100 times and then used conveniently, quickly and efficiently in a rinsing way, a sprinkling way, a smearing way or the like. The fruit preservative has the advantages as follows: firstly, Chinese herbal medicines such as enteromorpha prolifera, clove and cinnamon, which are safe and have no side effect, are selected as the raw materials so as to meet people's demands for healthy and safe fruit preservatives with no side effect; secondly, enteromorpha prolifera polysaccharide with an antibacterial effect is modified through selenylation so that the antibacterial effect of the enteromorpha prolifera polysaccharide is further improved; thirdly, the enteromorpha prolifera polysaccharide selenide and the Chinese herbal medicine ethanol extracts are integrated, so that the fruit preservative product has a wide anti-bacteria range and a high antibacterial capability and can effectively prolong the fruit preservation time.

Description

technical field [0001] The invention relates to a natural fruit fresh-keeping agent based on the comprehensive utilization of enteromorpha and a preparation method thereof, belonging to the field of food additives. Background technique [0002] Enteromorpha is a large green algae, commonly known as moss strips, green sea moss, etc., is an algae plant of the genus Enteromorpha in the order Ulvaceae of Chlorophyta phylum Ulvaceae. Moss and so on. Enteromorpha, as a natural wild green algae in coastal flats, has a particularly strong natural reproductive ability and a huge output. In recent years, large-scale sudden green tides of Enteromorpha have continuously erupted in my country's coastal waters, which have caused serious damage to human sea activities (maritime sports, marine fisheries, ship traffic, etc.), seawater utilization, coastal landscape, marine ecological environment and people's normal life. Serious impact. Enteromorpha is rich in nutrients, and polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
Inventor 董书阁侯文燕董静静段智岗侯文杰代书强
Owner 广西贺州市正丰现代农业股份有限公司
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