Preparation method of high-emulsion stable soy proteins
A technology of soybean protein and emulsification stability, applied in the direction of vegetable protein processing, etc., to achieve the effects of short production cycle, low production cost, and accelerated dispersibility
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specific Embodiment approach 1
[0018] Embodiment 1: This embodiment prepares high emulsification stable soybean protein according to the following method:
[0019] (1) Ultrasonic modification: Prepare soybean protein into a soybean protein solution with a mass concentration of 0.5-2% (prepared with a 0.04M phosphate buffer solution with a pH of 7.0), at a temperature of 20-30°C and a power of 400- Under the condition of 640W, ultrasonically modify soybean protein for 5-10 minutes;
[0020] (2) Alkylation modification: use the soybean protein solution after step (1) ultrasonic modification as raw material, adopt alkylation reaction to modify it, the specific steps are as follows: to the soybean protein solution after ultrasonic modification Add benzaldehyde and freshly prepared NaBH sequentially to 3 CN solution, NaBH 3 The concentration of CN solution is 6mg / ml (NaBH 3 CN solution adopts the 0.04M phosphate buffer saline preparation of pH=7.0), and its addition is 0.1-0.3% of soybean protein solution qua...
specific Embodiment approach 2
[0023] Specific embodiment two: this embodiment prepares high emulsification type soybean protein according to the following method:
[0024] (1) Ultrasonic modification: use 0.04M phosphate buffer solution with pH=7.0 to prepare a soybean protein solution with a mass concentration of 1%. Ultrasonic modification for 6 minutes;
[0025] (2) Alkylation modification: Add benzaldehyde and 0.15% freshly prepared 6mg / ml NaBH successively to the soybean protein solution in step (1) 3 CN solution (NaBH 3 CN solution is prepared by using 0.04M phosphate buffer solution with pH=7.0), benzaldehyde and NaBH 3 The mass ratio of CN solution added is 1:1, react at 20-30°C for 60 minutes, and shake gently every 15 minutes;
[0026] (3) Dialysis: Dialyze the soybean protein solution in step (2) at 4°C;
[0027] (4) Drying: The soybean protein liquid in step (3) is spray-dried to obtain a highly emulsifying stable soybean protein product, with an emulsifying property of 0.978 and an emulsif...
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