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Extraction method of microalgae oil and content detecting method for triglyceride in microalgae

A technology of microalgae oil and extraction method, which is applied in the direction of oil/fat production, measuring device, fat production, etc., to achieve the effects of low power consumption, resource saving, and short wall breaking time

Active Publication Date: 2015-07-08
JIANGSU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Generally speaking, the selected organic solvent should strive to obtain a high oil yield during the extraction process while avoiding the damage of the solvent to the human body. Studies have shown that it is difficult to extract the intracellular oil of microalgae with a single solvent

Method used

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  • Extraction method of microalgae oil and content detecting method for triglyceride in microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (Example 1, the extraction method of microalgae oil)

[0045] The extraction method of the microalgae oil of the present embodiment comprises the following steps:

[0046] 1. Get fresh microalgae grown to the end of the logarithm (the microalgae in this embodiment is the diatom Chaetoceros, Chaetoceros gracilis , provided by the University of Minnesota, USA) 1000mL of culture medium was centrifuged at 8000r / min for 15 minutes, the supernatant was removed and the obtained microalgae were freeze-dried, and the weight change of the microalgae was measured every 12h during the freeze-drying process. After constant weight ( In this example, after 72 hours of freeze-drying, the microalgae powder has a constant weight) Weigh 0.2 g of the algae powder into a 15 mL test tube.

[0047] ② Prepare 100 mL of a mixed solution with tetrahydrofuran: chloroform: n-hexane at a volume ratio of 1:1:1 for use.

[0048] ③Use a pipette to pipette 5mL of the mixed solution prepared in step ...

Embodiment 2

[0055] (Example 2, the extraction method of microalgae oil)

[0056] The extraction method of the microalga oil of the present embodiment all the other are identical with embodiment 1, difference is:

[0057] When preparing the mixed solution in step ②, the volume ratio of tetrahydrofuran: chloroform: n-hexane is 1:1.5:1. The quality of the oil that step 5. extracts is 0.076g.

Embodiment 3

[0058] (Example 3, the extraction method of microalgae oil)

[0059] The extraction method of the microalga oil of the present embodiment all the other are identical with embodiment 1, difference is:

[0060] When preparing the mixed solution in step ②, the volume ratio of tetrahydrofuran: chloroform: n-hexane is 1:0.8:1. The quality of the oil that step 5. extracts is 0.079g.

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Abstract

The invention discloses an extraction method of microalgae oil and a content detecting method for triglyceride in microalgae. During extraction of microalgae oil, a mixed solvent is adopted to be mixed with microalgae powder, and then the ultrasonic wall breaking extraction is implemented; the microalgae wall breaking rate is up to 99%; the wall breaking time is short; and the efficiency of extracting the triglyceride in the microalgae in the whole process is high. The content detecting method for the triglyceride in the microalgae adopts a gas phase method; the gas phase method can effectively and accurately detect the content of triglyceride.

Description

technical field [0001] The invention relates to a method for extracting oil in microalgae cells and a method for detecting the content of triglyceride contained in microalgae cells. Background technique [0002] The world's crude oil production continues to decrease and is expected to drop to 7.5 billion barrels per year by 2018, which is only equivalent to the crude oil production in 1950. With the increasing depletion of petroleum resources, global energy shortage and environmental pollution, the development of green and clean biofuels to replace traditional fossil fuels has become an international research hotspot. [0003] Biodiesel refers to organic fatty acid esters obtained from vegetable oils, animal fats, and microbial intracellular oils through transesterification with short-chain alcohols. As a renewable alternative energy source, its environmental friendliness and renewability make it highly competitive force. Among the many raw materials for biodiesel, microal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/04C11B1/10G01N30/02G01N1/28
Inventor 梁国斌刘维平朱政滔
Owner JIANGSU UNIV OF TECH