Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quality control method and application of baccharis type propolis

A Dionysus chrysanthemum and propolis technology, which is applied in the field of quality control of Dionysus type propolis, can solve the problems that it is difficult to reflect the biological activity of Brazilian Dionysus type propolis, and achieve high sensitivity, good separation effect and strong feasibility Effect

Active Publication Date: 2014-02-19
ZHEJIANG UNIV +1
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, referring to China’s national propolis standards, it is difficult to reflect the biological activity of Brazilian Dionysus propolis, and even poplar propolis or even poplar gum is used as Brazilian Dionysus propolis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quality control method and application of baccharis type propolis
  • Quality control method and application of baccharis type propolis
  • Quality control method and application of baccharis type propolis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substance 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0024] Take a certain amount of propolis samples of Dionysus genus to be tested, freeze, pulverize and mix, take 0.25g, and use 80% ethanol for ultrasonic extraction. Constant volume, after filtering through a microporous membrane, use high performance liquid chromatography to measure.

[0025] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0026] The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient is eluted by volume ratio. The elution program is: 0-10 min, methanol 15-25%; 10-25 min,...

Embodiment 2

[0029] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substances 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0030] A certain amount of poplar-type propolis and poplar gum samples were taken for freezing, pulverization and mixing, and 0.25 g of each was used for ultrasonic extraction with 70% ethanol. The ratio of solid to liquid was 1:45. 70% ethanol to constant volume, filtered through a microporous membrane, and then determined by high performance liquid chromatography.

[0031] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0032]The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient is elut...

Embodiment 3

[0035] Weigh chlorogenic acid, p-coumaric acid, caffeic acid, rutin and Artepillin C reference substances 10, 10, 10, 40, 10 mg respectively, and dilute to 10 mL with methanol. Then take 0.5, 0.2, 0.5, 0.25, 4 mL respectively and dilute to 10 mL with methanol as the standard mixture, and analyze by HPLC.

[0036] Take a certain amount of eucalyptus-type propolis for testing, freeze and pulverize and mix, take 0.25 g, and use 90% ethanol for ultrasonic extraction, the ratio of solid to liquid is 1:30, ultrasonic for 30 min, extract twice, and use 90% ethanol to volume, After filtering through a microporous membrane, it is determined by high performance liquid chromatography.

[0037] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL.

[0038] The mobile phase is: pure methanol and 1% acetic acid water mixture by volume ratio, and the binary gradient ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a quality control method for baccharis type propolis. The method comprises the following steps: taking chlorogenic acid, p-cumaric acid, caffeic acid, rutin and Artepillin C as reference indexes, and detecting chlorogenic acid, p-cumaric acid, caffeic acid, the rutin and the Artepillin C in the propolis according to a high performance liquid chromatography method to really identify the authenticity of the baccharis type propolis. The method for identifying the authenticity of the baccharis type propolis has the advantages of high accuracy, good sensitivity, shorter time, low cost and the like, solves the technical difficult problems of authenticity identification and quality control of the baccharis type propolis, can be used for qualitatively identifying the baccharis type propolis and effectively distinguishing if the rutin is added, and is effective.

Description

technical field [0001] The invention belongs to the field of bee products and relates to a method for quality control of Dionysus type propolis. Background technique [0002] Propolis has a wide range of biological activities, mainly antibacterial, antifungal, antiprotozoal, antiviral, antitumor, immunomodulatory, anticancer and antioxidant activities. Propolis has been widely used in health food, beverage and medicine. [0003] Brazil is located in the Amazon Basin of South America. It has the largest virgin forest in the world, and its plant species ranks first in the world. It is known as the "treasure house of plants" in the world. The chemical composition and pharmacological activity of propolis from different geographical sources in Brazil are quite different. Current research shows that propolis in Brazil can be divided into 13 types according to geographical origin, chemical composition and plant source, among which green propolis in southeast Brazil is the most re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张翠平胡福良黄帅刘嘉
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com