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Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs

A timosaponin and anti-tumor technology, which is applied in the field of pharmacy, can solve the problems of anti-tumor and anti-cancer, and achieve the effect of expanding the range of options

Inactive Publication Date: 2014-02-26
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern natural medicinal chemistry and pharmacology studies have shown that Anemarrhena contains steroidal saponins, flavonoids, lignans, polysaccharides, sterols, and fatty oils, among which saponins are the main components. Previous and current studies on the pharmacological activity of Anemarrhena , are basically concentrated in the fields of anti-aging (zhimoning), anti-depression (zhimosides), treatment of Alzheimer's disease (timosaponin Q), anti-type 2 diabetes (mangiferin), etc. They all protect cells or promote cell proliferation, but there are very few studies on anti-tumor and anti-cancer aspects

Method used

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  • Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs
  • Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs
  • Application of timosaponin AIII in anemarrhena to preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. MTT method was used to determine the growth inhibitory effect of timosaponin AⅢ on HeLa, HepG2, MCF-7, HCT116, HT1080, T475-S2, K562, HL60, U937 and A549 cells.

[0031] Experimental Materials:

[0032] 1. Cells

[0033] HeLa cells (cultured in RPMI-1640 medium), HepG2 cells (cultured in RPMI-1640 medium), MCF-7 cells (cultured in RPMI-1640 medium), HCT116 cells (cultured in RPMI-1640 medium), HT1080 cells (cultured in RPMI -1640 medium), A375-S2 cells (MEM medium), K562 cells (RPMI-1640 medium), HL60 cells (RPMI-1640 medium), U937 cells (RPMI-1640 medium) , A549 cells (cultured in DMEM medium) were purchased from American Type Culture Collection (ATCC, Manasas, MD, USA). Inoculate in RPMI-1640 medium or MEM medium or DMEM medium containing 10% fetal bovine serum at 37°C in 5% CO 2 cultured in an incubator.

[0034] 2. Drugs and reagents

[0035] Timosaponin AⅢ was dissolved in dimethyl sulfoxide. Under sterile conditions, after dissolving DMSO, dilute ...

Embodiment 2

[0053] Example 2, MTT method to measure the growth inhibitory effect of timosaponin AⅢ, 5-fluorouracil and paclitaxel on hPBMC

[0054] Experimental Materials:

[0055] 1. Cells

[0056] Human peripheral blood mononuclear cells (hPBMC) were collected from three healthy adult volunteers by the Shenyang Military Region Army General Hospital entrusted by the Sino-Japanese Medical Research Institute of Shenyang Pharmaceutical University.

[0057] 2. Drugs and reagents

[0058] Timosaponin AⅢ was dissolved in dimethyl sulfoxide. Under sterile conditions, after dissolving DMSO, dilute with RPMI-1640 culture solution or MEM culture solution or DMEM culture solution to the required concentration (DMSO≤2.5‰).

[0059] 5-Fluorouracil and paclitaxel were purchased from China Institute for the Control of Pharmaceutical and Biological Products and dissolved in dimethyl sulfoxide. Under sterile conditions, after dissolving DMSO, dilute with RPMI-1640 culture solution or MEM culture solu...

Embodiment 3

[0075] Example 3. Determination of growth inhibitory effects of timosaponin AⅢ, 5-fluorouracil and paclitaxel on T475-S2 cells by MTT method (repeated screening)

[0076] Experimental Materials:

[0077] 1. Cells

[0078] A375-S2 cells (cultured in MEM medium) were purchased from American Type Culture Collection (ATCC, Manasas, MD, USA). Inoculated in MEM medium at 37°C, 5% CO 2 cultured in an incubator.

[0079] 2. Drugs and reagents

[0080] Timosaponin AⅢ was dissolved in dimethyl sulfoxide. Under sterile conditions, after dissolving DMSO, dilute with RPMI-1640 culture solution or MEM culture solution or DMEM culture solution to the required concentration (DMSO≤2.5‰).

[0081] 5-Fluorouracil and paclitaxel were purchased from China Institute for the Control of Pharmaceutical and Biological Products and dissolved in dimethyl sulfoxide. Under sterile conditions, after dissolving DMSO, dilute with RPMI-1640 culture solution or MEM culture solution or DMEM culture solutio...

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Abstract

The invention discloses application of timosaponin AIII in anemarrhena to preparation of antitumor drugs, which belongs to the field of pharmacology. Pharmacological experiments prove that timosaponin AIII has broad-spectrum strong antineoplastic activity, especially strong and stable cytotoxicity on T475-S2 cells; timosaponin AIII has stronger capacity in killing T475-S2 cells compared with 5-Fu and paclitaxel; the IC50 value of timosaponin AIII is 6.97 +- 0.72 mu M after 24 h, the concentration of used timosaponin AIII is 6 mu M, timosaponin AIII can induce apoptosis and autophagy of T475-S2 cells, the effect of apoptosis is dominating, so timosaponin AIII can be used for preparing drugs for preventing and treating tumors.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to the application of timosaponin AⅢ in the preparation of antitumor drugs, in particular to the application of timosaponin AⅢ through death receptor pathways, namely Fas and FADD pathways, and endogenous pathways, namely mitochondrial and Cyto.c pathways Induces apoptosis and autophagy of A375-S2 cells for the preparation of antitumor drugs. Background technique [0002] In recent years, due to factors such as intensified competition in modern society, increased living pressure, and severe environmental pollution, malignant tumors have become one of the serious diseases that endanger human life and health, and have become the second "killer disease" that seriously threatens human health! The role of natural medicines in cancer treatment has attracted much attention. Whether we can find medicines and treatment methods that can kill tumor cells and have less toxic side effects is of great signi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P35/02
Inventor 宋少江池岛乔夏明钰李玲芝徐雷殷缘
Owner SHENYANG PHARMA UNIVERSITY
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