Method for improving water-retaining property of myofibrillar protein gel of frozen fish
A technology of myofibrillar protein and fish meat, which is applied in the direction of fish protein components, animal protein processing, protein food ingredients, etc., can solve the problems of poor water retention and poor taste of fish meat, and achieve the improvement of product taste, nutrition and performance. Good efficacy and controllability
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[0026] like figure 1 Shown is a method for improving the water retention of frozen fish myofibrillar protein gel, comprising the following steps:
[0027] (1) Squid pretreatment: Remove the internal organs and fish eyes of the squid, wash them, cut into pieces, and mash them into minced fish with a tissue grinder, and set aside.
[0028] (2) Enzymolysis: Mix minced fish meat and deionized water according to the ratio of material to liquid 1:1-4, stir and mix evenly, adjust the pH to 7-8 with 6mol / L NaOH and 6mol / L HCl, add papain, animal protease or medium Protease was hydrolyzed at 37-50°C for 3-5h, and the pH value of the reaction solution was adjusted with 6mol / L NaOH and 6mol / L HCl every 30min, and the enzyme was inactivated by heating at 95-100°C for 10-15min after the reaction. After the reaction, the enzyme must be inactivated to prevent the transition of enzymatic hydrolysis, resulting in the exposure of hydrophobic amino acid residues or hydrolysis of the peptide...
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