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Preparation method of graphene/hemoglobin composite film modified electrode

A technology of hemoglobin and composite thin films, applied in the direction of material electrochemical variables, material analysis by electromagnetic means, measurement devices, etc., can solve the problems of weak interaction, poor dispersion, and restrictions on the research and application of graphene composite materials, and achieve the goal of making The effect of simple process, low cost and good sensitivity

Inactive Publication Date: 2014-03-05
JIANGNAN UNIV
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Problems solved by technology

However, due to the high chemical stability of graphene, its surface is in an inert state, and the interaction with other media is weak, and there is a strong van der Waals force between graphene sheets, which is prone to agglomeration, making it difficult to dissolve in water and Commonly used organic solvents have poor dispersion, which greatly limits the further research and application of graphene composites

Method used

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  • Preparation method of graphene/hemoglobin composite film modified electrode

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Embodiment 1

[0016] In the first step, add 30 mg graphene oxide to a 250 mL flask, add DMF / H 2 O (V:V=9:1) solution 50mL, stirred, sonicated for 30min. Add 0.0248g NaBH to the flask 4 , 80°C oil bath for 4 hours, after cooling to room temperature, 10g of acrylic acid and 40mL of H 2 O, stirred for 30min. into high-purity N 2 30min, slowly drop 100mg (NH 4 ) 2 S 2 o 8 (dissolved in 80mL deionized water), ultrasonic, 60°C oil bath, stirring. After 48h, cool to room temperature and wash with 200mLH 2 After diluting with O, sonicate for 1 h and centrifuge to obtain polyacrylic acid-modified graphene.

[0017] In the second step, 20 mg of hemoglobin was dissolved in 10 mL of water to obtain a 2 mg / mL aqueous hemoglobin solution.

[0018] In the third step, the treated glassy carbon electrode is soaked in the hemoglobin aqueous solution for 20 minutes, then rinsed with a large amount of deionized water, and dried with nitrogen gas, so that the surface of the obtained glassy carbon elec...

Embodiment 2

[0022] In the first step, add 50 mg graphene oxide to a 250 mL flask, add DMF / H 2 O (V:V=9:1) solution 80mL, stirred, sonicated for 30min. Add 0.0248g NaBH to the flask 4 , 80°C oil bath for 4 hours, after cooling to room temperature, 20g of benzenesulfonic acid and 60mL of H 2 O, stir for 30min. into high-purity N 2 30min, slowly dropwise add 0.2g (NH 4 ) 2 S 2 o 8 , ultrasonic, 60 ° C oil bath, stirring. After 48h, cool to room temperature and wash with 300mL H 2 After diluting with O, sonicate for 1 h, and centrifuge to obtain polybenzenesulfonic acid-modified graphene.

[0023] In the second step, 10 mg hemoglobin was dissolved in 10 mL water to obtain a 1 mg / mL hemoglobin aqueous solution.

[0024] In the third step, the treated gold electrode is soaked in the hemoglobin aqueous solution for 20 minutes, then rinsed with a large amount of deionized water, and dried with nitrogen gas, so that the surface of the obtained gold electrode is positively charged.

[00...

Embodiment 3

[0028] In the first step, add 50 mg graphene oxide to a 250 mL flask, add DMF / H 2 O (V:V=9:1) solution 80mL, stirred, sonicated for 30min. Add 0.0248g NaBH to the flask 4 , 80°C oil bath for 4 hours, after cooling to room temperature, 20g of acrylic acid and 60mL of H 2 O, stirred for 30min. into high-purity N 2 30min, slowly dropwise add 0.2g (NH 4 ) 2 S 2 o 8 , ultrasonic, 60 ° C oil bath, stirring. After 48h, cool to room temperature and wash with 300mLH 2 After diluting with O, sonicate for 1 h, and centrifuge to obtain polybenzenesulfonic acid-modified graphene.

[0029] In the second step, 10 mg hemoglobin was dissolved in 10 mL water to obtain a 1 mg / mL hemoglobin aqueous solution.

[0030] In the third step, soak the treated ITO electrode in the hemoglobin aqueous solution for 30 minutes, then rinse it with a large amount of deionized water, and dry it with nitrogen gas, so that the surface of the obtained ITO electrode is positively charged.

[0031] In the...

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Abstract

The invention relates to a preparation method of a graphene / hemoglobin composite film based on a layer by layer self-assembly technology, which belongs to the technical field of preparation of composite material modified electrodes. The preparation method provided by the invention comprises the following steps: soaking a treated electrode in modified graphene aqueous dispersion for a period of time, taking out the electrode, cleaning the electrode through deionized water, then, soaking the electrode in a hemoglobin aqueous solution for a period of time, taking out and cleaning the electrode, and repeating the process to form a uniform graphene / hemoglobin film having a regular layered structure on the surface of the electrode. The graphene / hemoglobin modified electrode prepared by the method is controllable in structural performance, good in sensitivity, reproducibility and stability, wide in linear detection range, low in lower detection limit, simple in preparation process, low in cost and easy to operate.

Description

Technical field: [0001] The invention belongs to the field of preparation of modified electrodes based on composite materials, and is characterized in that a graphene / hemoglobin multilayer composite film is prepared by a layer-by-layer self-assembly method, and is used in the preparation of modified electrodes. Background technique: [0002] In recent years, the electrode modified by composite material is a hotspot in the field of sensors, and more and more people pay more and more attention to it. [0003] Hemoglobin is a protein with heme as the prosthetic group. It is a kind of tetramer composed of two α and two β subunits, which are responsible for transferring electrons, transporting oxygen, and decomposing H in organisms. 2 o 2 and other important functions related to energy metabolism in organisms. Since hemoglobin has a catalytic effect on peroxidase and cytochrome, and it is cheap and easy to obtain, it is considered to be an ideal model of protein with heme molec...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327
Inventor 罗静朱正意刘晓亚
Owner JIANGNAN UNIV
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