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Induction method of gulfweed regeneration plant

A technology for regenerating plants and Sargassum, applied in the field of plant tissue culture, can solve the problems of immature tissue culture technology and achieve the effect of enriching germplasm resources

Active Publication Date: 2014-03-12
太湖县舜华菌业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue culture technology of Sargassum is still immature, and there is no successful method to stably form a large number of callus or clustered buds.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The rhizoid root of Sargassum was used as explant, cleaned, treated with 0.2% povidone for 3 minutes, then sterilized in 3% sodium hypochlorite solution for 5 minutes, and sterilized in 0.1% mercuric chloride for 3 minutes; the sterilized The explants were cleaned with sterile filtered seawater and cut into sections with a length of 0.3-0.5mm; the sections were inoculated in solid PES medium, and 1L of PES medium was added with 0.1mg of ZT, 1mg of IAA, 1g of ascorbic acid, and sucrose 30g, 7g agar; under the conditions of light intensity 4000-5000Lx, light time 8h, and temperature 18℃, continue to cultivate for 28d until bud formation; inoculate 100 pieces of rhizoid cuts in total, and 20 out of 100 pieces of tissue pieces Buds formed on the implants, and the rate of bud induction was 20%.

Embodiment 2

[0019] The rhizoid root of Sargassum was used as explant, cleaned, treated with 0.2% povidone for 3 minutes, then sterilized in 3% sodium hypochlorite solution for 5 minutes, and sterilized in 0.1% mercuric chloride for 3 minutes; the sterilized The explants are washed with sterile filtered seawater and cut into sections with a length of 0.3-0.5mm; the sections are inoculated in solid PES medium, and 1L of PES medium is added with 1mg of ZT, 2mg of IAA, 3g of ascorbic acid, and 30g of sucrose , agar 7g; continue to cultivate for 28 days under the conditions of light intensity of 4000-5000Lx, light time of 8h, and temperature of 18°C ​​until bud formation; a total of 100 rhizoid cuts were inoculated, and 40 of the 100 tissue blocks were explanted Buds were formed on the body, and the induction rate of buds was 42%.

Embodiment 3

[0021] The rhizoid root of Sargassum was used as explant, cleaned, treated with 0.2% povidone for 3 minutes, then sterilized in 3% sodium hypochlorite solution for 5 minutes, and sterilized in 0.1% mercuric chloride for 3 minutes; the sterilized The explants were cleaned with sterile filtered seawater and cut into sections with a length of 0.3-0.5mm; the sections were inoculated in solid PES medium, and 1L of PES medium was added with 0.5mg of ZT, 1mg of IAA, 2g of ascorbic acid, and sucrose 30g, 7g of agar; under the conditions of light intensity 4000-5000Lx, light time 8h, temperature 18°C, continue to cultivate for 28d until bud formation; inoculate a total of 100 rhizoid cut pieces, 30 out of 100 tissue pieces Buds formed on the implants, and the rate of bud induction was 30%.

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Abstract

The invention discloses an induction method of gulfweed regeneration plant. The induction method of the gulfweed regeneration plant comprises the following steps: preparing a sterile explant, inoculating the explant to an induction culture medium and inducing callus to form at an induction temperature of 15-20 DEG C with a light intensity of 4000-5000Lx and a light period of 8 hours; processing gulfweed rhizoid in 0.2% povidone for 3 minutes, and then sterilizing in a 3% sodium hypochlorite solution for 5 minutes, sterilizing in 0.1% mercury bichloride for 3 minutes so as to obtain a sterile survival explant; adding 0.1-1mg of ZT, 1-2mg of IAA (Indole Acetic Acid), 1-3g of ascorbic acid, 30g of cane sugar and 7g of agar in a 1LPES culture medium, sterilizing at a high temperature and a high pressure for 10-20 minutes to bud the gulfweed.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Sargassum. Background technique [0002] Sargassum is a large economical brown algae with a maximum length of more than 7 m. The natural algae field formed by Sargassum contains rich biological resources. It is a good breeding place for fish, shrimps and crabs, and has the highest marine productivity. One of the ecosystems. Sargassum is rich in carbohydrates, lipids, proteins, vitamins, minerals and a variety of trace elements necessary for the human body, and its development and utilization prospects are very broad. Sargassum is a perennial seaweed. Due to its fast growth rate, large biomass and important ecological service functions in shallow sea ecological environments, it can be used as a "blue carbon sink", algae farm reconstruction, ecosystem environmental restoration, artificial fish reefs and It is one of the important constr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张亚峰
Owner 太湖县舜华菌业发展有限公司
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