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Method for extracting and preparing active ingredients of fish roes

A technology of active ingredients and fish roe, which is applied in the field of extraction and preparation of fish roe active ingredients, can solve the problems of not giving full play to the economic value of fish roe and backward processing technology, and achieve remarkable social and economic benefits

Inactive Publication Date: 2014-03-12
郑州市中食农产品加工研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the relatively backward processing technology at present, most enterprises only carry out simple processing, and do not give full play to the economic value of fish roe

Method used

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  • Method for extracting and preparing active ingredients of fish roes

Examples

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Effect test

Embodiment 1

[0020] A technology for extracting and preparing active ingredients of fish roe, comprising the following steps: (1) collecting salmon roe, beating and then putting them into a subcritical extraction kettle, and using CO 2 As an extractant, extract under the conditions of temperature 35° C., pressure 10 MPa, extraction time 100 min, and flow rate 35 L / h, to obtain fish seed oil rich in DHA and EPA. (2) Add 3% sodium chloride solution to the extracted fish roe, and extract caviar globulin under the extraction conditions of temperature 30°C, time 1h, and solid-liquid ratio 1:8. (3) After caviar globulin extraction is completed, centrifuge, and the supernatant is concentrated and desalted through the membrane to obtain caviar globulin concentrate. (4) Add water and protease to the precipitated part after globulin extraction for enzymolysis, and three-step enzymolysis with three proteases to prepare bioactive peptides. The first step of enzymatic hydrolysis: adding 0.5% of alkali...

Embodiment 2

[0022] A technology for extracting and preparing active ingredients of fish roe, comprising the following steps: (1) collecting salmon roe, beating and then putting them into a subcritical extraction kettle, and using CO 2 As an extractant, extraction is carried out at a temperature of 40° C., a pressure of 8 MPa, an extraction time of 200 min, and a flow rate of 20 L / h to obtain fish seed oil rich in DHA and EPA. (2) Add 3% sodium chloride solution to the extracted fish roe, and extract caviar globulin under the extraction conditions of temperature 40°C, time 3h, and solid-liquid ratio 1:12. (3) After caviar globulin extraction is completed, centrifuge, and the supernatant is concentrated and desalted through the membrane to obtain caviar globulin concentrate. (4) Add water and protease to the precipitated part after globulin extraction for enzymolysis, and three-step enzymolysis with three proteases to prepare bioactive peptides. The first step of enzymatic hydrolysis: addi...

Embodiment 3

[0024] A technology for extracting and preparing active ingredients of fish roe, comprising the following steps: (1) collecting salmon roe, beating and then putting them into a subcritical extraction kettle, and using CO 2As an extractant, extraction is carried out at a temperature of 50° C., a pressure of 6 MPa, an extraction time of 300 min, and a flow rate of 60 L / h to obtain fish seed oil rich in DHA and EPA. (2) Add 3% sodium chloride solution to the extracted fish roe, and extract caviar globulin under the extraction conditions of temperature 25°C, time 5h, and solid-liquid ratio 1:15. (3) After caviar globulin extraction is completed, centrifuge, and the supernatant is concentrated and desalted through the membrane to obtain caviar globulin concentrate. (4) Add water and protease to the precipitated part after globulin extraction for enzymolysis, and three-step enzymolysis with three proteases to prepare bioactive peptides. The first step of enzymatic hydrolysis: addin...

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Abstract

The invention relates to a method for extracting and preparing active ingredients of fish roes. The method comprises the following steps: (1) collecting and pulping the fish roes and placing in a subcritical extraction kettle, and extracting by using CO2 as an extraction agent to obtain fish roe oil rich in DHA and EPA; (2) extracting globulin in the extracted fish roes by a salt method; (3) centrifuging after the globulin of the fish roes is extracted, carrying out membrane concentration on the supernate and desalting to obtain a globulin concentrated solution of the fish roes; (4) adding water and protease into the sediment obtained after globulin extracting for enzymolysis, and preparing biological active peptide by enzymolysis through a three-step method; (5) carrying out solid-liquid separation on the feed liquid through a centrifugal machine after enzymolysis, and concentrating the supernate through a nanofiltration membrane and desalting to obtain biological active peptide concentrated solution of the fish roes; and (6) adding a certain amount of beta-cyclodextrin in the globulin concentrated solution of the fish roes and a biological active peptide concentrated solution of the fish roes, embedding under certain ultrasonic condition, and carrying out freeze drying or spray drying to obtain fish roe globulin and fish roe biological active peptide products.

Description

Technical field: [0001] The invention belongs to the field of food deep processing, and in particular relates to a technique for extracting and preparing active components of caviar. Background technique: [0002] my country has vast fishery waters and rich biological resources. The total area of ​​the four seas of the Bohai Sea, the Yellow Sea, the East China Sea and the South China Sea is 4.7 million km 2 , there are more than 3,000 species of marine life, including 1,694 species of fish that can be caught and farmed, and more than 150 species of large economic value. my country's jurisdiction area in the four major sea areas is nearly 3 million km 2 , the area of ​​shallow sea and tidal flats within the 15-meter isobath is 120,000 km 2 , about 16,200 km available for breeding 2 . The area of ​​the intertidal zone is 20,000 km 2 , the coastline of the mainland is as long as 18,000 km. In addition, there are more than 6,500 islands with a coastline of 14,000 km. 2 . ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/46C11B1/10
Inventor 黄继红惠明侯银臣冯军伟苏雪峰杨铭乾王文游倩倩刁大鹏
Owner 郑州市中食农产品加工研究院
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