Urine creatinine detection reagent strip and preparation method thereof

A technology for detecting reagents and urine creatinine is applied in the field of urine creatinine detection reagent strips and their preparation, which can solve problems such as background interference, and achieve the effects of simple preparation method, stable performance and accurate measurement results.

Active Publication Date: 2014-03-12
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is improved from the picric acid method, which effectively reduces the interference of false creatinine. However, during the production process of the test paper, the strong alkali solution under high temperature conditions not only degrades the test paper fiber, but also makes 3,5-dinitrobenzene Oxidative discoloration of formic acid causing background interference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment provides a urine creatinine detection reagent strip, which is prepared through the following steps:

[0026] (1) Prepare liquid A: put 32g of sodium hydroxide, 10g of polyethylene glycol, and 800mL of absolute ethanol into a volumetric flask, add water to 1000mL; test with pH test paper, the pH of liquid A is 13;

[0027] (2) Prepare liquid B: put 8g of 3,5-dinitrobenzoic acid and 0.08g of bromocresol green into a volumetric flask, add anhydrous methanol to 1000mL;

[0028] (3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 20°C to dry for 20 minutes;

[0029] (4) Immerse the dried filter paper in the B solution, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 20° C. for 20 minutes;

[0030] (5) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine creatinine detection reagent s...

Embodiment 2

[0032] This embodiment provides a urine creatinine detection reagent strip, which is prepared through the following steps:

[0033] (1) Prepare liquid A: put 48g of sodium hydroxide, 15g of polyethylene glycol, and 950mL of absolute ethanol into a volumetric flask, add water to 1000mL; test with pH test paper, the pH of liquid A is 14;

[0034] (2) Prepare liquid B: put 12g of 3,5-dinitrobenzoic acid and 0.12g of bromocresol green into a volumetric flask, add anhydrous methanol to 1000mL;

[0035] (3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 30°C to dry for 10 minutes;

[0036] (4) Immerse the dried filter paper in the B solution, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 40° C. for 10 minutes;

[0037] (5) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine creatinine detection reagent ...

Embodiment 3

[0039] This embodiment provides a urine creatinine detection reagent strip, which is prepared through the following steps:

[0040] (1) Prepare liquid A: put 32g of potassium hydroxide, 10g of polyethylene glycol, and 800mL of absolute ethanol into a volumetric flask, add water to 1000mL; test with pH test paper, the pH of liquid A is 13;

[0041] (2) Prepare liquid B: put 8g of 3,5-dinitrobenzoic acid and 0.08g of bromocresol green into a volumetric flask, add anhydrous methanol to 1000mL;

[0042] (3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 30°C to dry for 10 minutes;

[0043] (4) Immerse the dried filter paper in the B solution, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 40° C. for 10 minutes;

[0044] (5) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine creatinine detection reagen...

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Abstract

The invention discloses a urine creatinine detection reagent strip and a preparation method thereof. The detection reagent strip comprises a substrate and a filter paper sheet, wherein the filter paper sheet is prepared by respectively sufficiently immersing filter paper in a solution A and a solution B, taking out and drying; the solution A is a 80-95v / v% ethanol solution comprising 3.2-4.8 wt% of buffer solution and 1-1.5 wt% of surfactant; and the solution B is a methanol solution comprising 0.8-1.2% of 3,5-dinitrobenzoic acid and 0.008-0.012% of bromocresol green. The urine creatinine detection reagent strip disclosed by the invention can quickly react with creatinine in the urine under alkaline conditions; the creatinine concentration is 0-26.4 mmol / 24h, and the test paper has an obvious color gradation from light blue to brown which can be easily visually distinguished and mechanically read; and the preparation method is simple and easy to operate, and has the advantages of stable performance and accurate detection result.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a urine creatinine detection reagent strip and a preparation method thereof. Background technique [0002] Creatinine is the product of muscle metabolism in the human body, and it is urine creatinine when it is excreted with urine. Urinary creatinine mainly comes from the blood and is excreted from the body after being filtered by the glomerulus. The normal range of urine creatinine is 8.4~13.25mmol / 24h or 40~130mg / dl. The daily excretion of urine creatinine in normal people is very stable, basically not affected by food protein content and urine volume, when the urine creatinine value is significantly abnormal (increase or decrease), it reflects that the renal function has been damaged to a considerable extent. Therefore, clinically, by detecting changes in urine creatinine content, it is possible to control changes in diseases in a timely manner and play an important reference ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 陈名利白鹏利胡军
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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