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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and microbial strains, applied in the fields of application, bacteria, and microorganisms, can solve problems such as unsatisfactory immunity and ability to inhibit pathogenic bacteria, weak gastrointestinal adsorption ability, and difficult screening work, etc., to achieve strong Gastrointestinal absorption ability, good acid resistance and bile salt resistance, and the effect of improving immunity

Active Publication Date: 2014-03-19
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strains of probiotics have a very wide range of strains. Even the individual differences between different strains of the same genus are very large, so the screening work is very difficult. In the application of probiotics, the survival rate after being ingested into the human body is generally not high. The adsorption ability to the gastrointestinal tract is not strong, and the performance in enhancing human immunity and inhibiting pathogenic bacteria is not ideal

Method used

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  • Lactobacillus plantarum and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening and Preservation of Bacterial Strains

[0021] The Lactobacillus plantarum LP45 (YMC1005 or L45) of the present invention is obtained by separating and screening from traditional yogurt at Ulan Hada Gacha, Ulan Hada Sumu Station, Siziwang Banner, Siziwang Banner, Ulan Qab City, Inner Mongolia. The specific separation and identification steps are as follows:

[0022] The Inner Mongolia Siziwangqi yogurt sample was dissolved in a sterilized saline test tube, fully shaken and mixed, and then dipped in the mixed liquid with an inoculation loop and streaked on an MRS plate (37°C for 72h) to isolate the strain. Pick a single colony from the first-generation plate with an inoculation loop, streak culture on a new MRS plate (37°C for 72 hours), and purify. Purify 2 more times in the same way until the final plate has the same form of colonies. Pick a single colony and inoculate it in MRS liquid medium for 18 hours at 37°C for storage and identification.

...

Embodiment 2

[0025] Example 2 Gastrointestinal Survival Test

[0026] Artificial digestive juice experiments (gastric acid resistance and bile acid resistance) were used to study the survival of Lactobacillus plantarum LP45 (YMC1005) in the human intestinal environment.

[0027] 1. Acid resistance test

[0028] Lactobacillus plantarum LP45 (YMC1005 or L45) was activated from cryopreservation tubes, and after 3 activations (24 hours for the first activation, 14 hours for the second activation, and 18 hours for the third activation), 3% was inoculated in 4 pH3.0 tubes (using Hydrochloric acid to adjust the pH) liquid MRS medium test tube, each test tube is filled with 10ml of liquid MRS medium. Take one test tube of liquid MRS medium with pH 3.0 inoculated with 3% to measure the number of viable bacteria at 0h; the other three test tubes of liquid MRS medium with pH 3.0 with 3% inoculated are used to test the OD600 of 4h. Two counts of viable bacteria were measured.

[0029] Survival rate...

Embodiment 3

[0035] Example 3 Gastrointestinal tract adsorption capacity test

[0036] LP45 (YMC1005 or L45) strain was used to adsorb Caco-2 cells to study the adsorption ability of LP45 (YMC1005 or L45) strain to gastrointestinal epithelial cells, and it was found that the adhesion force of LP45 (YMC1005 or L45) was 72.

[0037] The specific operation steps are:

[0038] 1. After treating the human intestinal cell line Caco-2 with 1ml 0.05% insulin for 5 minutes, the cells were gently patted and distributed in a 96-well culture plate. Add 10,000 cells to each well, and change 200μl of fresh DMEM every day. Medium.

[0039] 2. Lactobacillus plantarum LP45 (YMC1005 or L45) was activated twice with MRS, inoculated into 5ml MRS broth for cultivation, and after 24 hours, take 1ml and centrifuge at 6000rpm for 10 minutes, wash twice with PBS (pH 7.2) to obtain lactic acid bacteria bacteria liquid. Add 20 μl of lactic acid bacteria liquid to the cells in step 1, then incubate for 1 hour to a...

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Abstract

The invention relates to lactobacillus plantarum and application thereof, specifically to novel lactobacillus plantarum LP45 (YMC1005) with an accession number of CGMCC No. 8072. The invention further relates to application of the lactobacillus plantarum LP45 in reduction of cholesterol, improvement of immunity and inhibition of harmful bacteria to intestinal tracts.

Description

technical field [0001] The invention relates to a new strain of Lactobacillus, more precisely, the invention describes a new strain LP45 of Lactobacillus plantarum, which belongs to the field of biotechnology. Background technique [0002] Probiotics are of great help to human health. Scientists have conducted decades of research on them, and it has been proven that they play a role in gastrointestinal regulation, anti-oxidation, lowering cholesterol, preventing gastrointestinal cancer, and immune regulation. Works great. However, the strains of probiotics have a very wide range of strains. Even the individual differences between different strains of the same genus are very large, so the screening work is very difficult. In the application of probiotics, the survival rate after being ingested into the human body is generally not high. The adsorption ability to the gastrointestinal tract is not strong, and the performance in enhancing human immunity and inhibiting pathogenic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/00A61P31/04A61P3/06A61P37/04A23L1/29C12R1/25A23L33/00A61K35/747
Inventor 赵林森龄南葛春美马新颖齐世华
Owner 河北一然生物科技股份有限公司
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