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Cultivation medium for test-tube seedling of detoxified strawberry

A test-tube seedling and culture medium technology is applied in the field of detoxified strawberry test-tube seedling rejuvenation medium, which can solve the problems of unsustainable production, disordered growth potential and weakening of hormones, and achieve the effects of preventing bacterial infection and solving rejuvenation.

Inactive Publication Date: 2014-03-26
SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Repeated subculture of detoxified strawberry tissue culture test-tube plantlets will cause two problems: first, due to the accumulation of hormones, the endogenous hormones of the test-tube plantlets will be disturbed, the growth potential will be weakened, and the quality will decline, such as seedling degradation; In the process, it is easy to cause bacterial infection caused by improper operation, and with the proliferation of subcultures, the bacterial infection continues to spread, making the production unsustainable
[0006] In the event of degradation of virus-free strawberry seedlings or large-scale bacterial infection, the conventional solution is to reselect the female parent seedlings to collect explants, disinfect the explants, strip the shoot tip for detoxification, shoot tip culture, and subculture. A long process, in actual production, the impact on the production schedule is fatal

Method used

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  • Cultivation medium for test-tube seedling of detoxified strawberry
  • Cultivation medium for test-tube seedling of detoxified strawberry

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Experimental program
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Effect test

Embodiment 1

[0019] A detoxified strawberry test-tube seedling rejuvenation medium, the formula is as shown in Table 2, the detoxified strawberry test-tube seedlings infected with bacteria and weak in growth potential are cut into individual plants, inoculated in the rejuvenation medium formula 1, at a culture temperature of 22-24 ℃, the light intensity is 1000-200Lux, and the daily light time is 10-12 hours under the culture conditions for 30-45 days to complete the first rejuvenation culture; then the rejuvenated tissue culture seedlings are cut off the old leaves and bases The root system was transferred to the rejuvenation medium formula 2, and the second rejuvenation culture was carried out. The formula 1 and the formula 2 were used at intervals. After repeated subculture for 3 to 4 times, it was inoculated into the original proliferation medium MS+6-BA0.1-0.5mg / L+white granulated sugar 30g / L, and the test tube seedlings recovered their growth potential, grew robustly, and had dark gre...

Embodiment 2

[0021] The virus-free strawberry test-tube seedlings infected with bacteria and weak in growth potential were cut into individual plants and inoculated into the rejuvenation medium formula 2. The culture temperature was 22-24 °C, the light intensity was 1000-200 Lux, and the daily light time was 10-12 Cultivated for 30 to 45 days under the culture conditions of 1 hour to complete the first rejuvenation culture; then cut off the old leaves and basal roots of the once rejuvenated tissue culture seedlings, transfer them to the rejuvenation medium formula 4, and carry out the second rejuvenation For cultivation, formula 3 and formula 4 are used at intervals. After repeated subculture for 3 to 4 times, it was inoculated into the original proliferation medium MS+6-BA0.1-0.5mg / L+white granulated sugar 30g / L, and the test tube seedlings recovered their growth potential, grew robustly, and had dark green leaves. The proportion of bacterial infections can be reduced by more than 60%.

Embodiment 3

[0023] Cut the detoxified strawberry test-tube seedlings infected with bacteria and with weak growth potential into individual plants, inoculated in the rejuvenation medium formula 3, at the culture temperature of 22-24°C, the light intensity of 1000-200Lux, and the daily light time of 10-12 Cultivated for 30 to 45 days under the culture conditions of 1 hour to complete the first rejuvenation culture; then cut off the old leaves and basal roots of the once rejuvenated tissue culture seedlings, transfer them to the rejuvenation medium formula 4, and carry out the second rejuvenation For cultivation, formula 3 and formula 4 are used at intervals. After repeated subculture for 3 to 4 times, it was inoculated into the original proliferation medium MS+6-BA0.1-0.5mg / L+white granulated sugar 30g / L, and the test tube seedlings recovered their growth potential, grew robustly, and had dark green leaves. The proportion of bacterial infections can be reduced by more than 60%.

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Abstract

The invention relates to a cultivation medium for test-tube seedling of detoxified strawberry. The cultivation medium is prepared by mixing a nutrient solution comprising macroelements, iron salts, trace elements and hormone with another material. The macroelements comprise 1100-1237.5 mg / L of ammonium nitrate, 1266.7-1425 mg / L of potassium nitrate, 113.3-127.5 mg / L of potassium dihydrogen phosphate, 246.7-277.5 mg / L of magnesium sulfate heptahydrate and 221.5-249.2 mg / L of calcium chloride. The iron salts comprise 27.8 mg / L of ferrous sulfate heptahydrate and 37.3 mg / L of disodium dihydrogen ethylenediaminetetraacetate. The above-mentioned compounds are mixed to prepare the nutrient solution. The material is vermiculite. The nutrient solution and the vermiculite are mixed according to a volume ratio of 1:1. A difficult problem of test-tube seedling degeneration caused by plant endogenous hormone disorder due to hormone accumulation in repeated subculturing processes of the test-tube seedling of the detoxified strawberry, and difficult problem of bacterial infection in repeated subculturing processes are overcome by the cultivation medium. The production cost is saved. An effective route for production in good time for the test-tube seedling of the detoxified strawberry is provided.

Description

technical field [0001] The invention relates to the technical field of tissue culture test-tube seedling rejuvenation, in particular to a detoxified strawberry test-tube seedling rejuvenation medium. Background technique [0002] Strawberry belongs to the Rosaceae Strawberry genus and is a perennial herb with more than 50 species known worldwide. It is a very popular fruit. The cultivation of strawberries began in Europe in the 14th century. The main producing area is Europe, followed by America and Asia. The average yield per mu is the highest in the United States and Canada. [0003] The characteristics of strawberry production in the United States and Canada are: first, all strawberries are planted with virus-free seedlings; my country's main production areas are distributed in the eastern coastal areas such as Liaoning, Hebei, Shandong, Jiangsu, Shanghai, Zhejiang, and of course, there are also production in Xinjiang and other places. The main methods of detoxificatio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈权肖建梅张承妹
Owner SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING