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A detoxified strawberry test-tube seedling rejuvenation medium

A test-tube seedling and culture medium technology, applied in plant cells, horticulture, botanical equipment and methods, etc., can solve the problems of unsustainable production, disordered growth potential and weakening of hormones, and achieve the effect of preventing bacterial infection and solving rejuvenation.

Inactive Publication Date: 2016-01-06
SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Repeated subculture of detoxified strawberry tissue culture test-tube plantlets will cause two problems: first, due to the accumulation of hormones, the endogenous hormones of the test-tube plantlets will be disturbed, the growth potential will be weakened, and the quality will decline, such as seedling degradation; In the process, it is easy to cause bacterial infection caused by improper operation, and with the proliferation of subcultures, the bacterial infection continues to spread, making the production unsustainable
[0006] In the event of degradation of virus-free strawberry seedlings or large-scale bacterial infection, the conventional solution is to reselect the female parent seedlings to collect explants, disinfect the explants, strip the shoot tip for detoxification, shoot tip culture, and subculture. A long process, in actual production, the impact on the production schedule is fatal

Method used

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  • A detoxified strawberry test-tube seedling rejuvenation medium
  • A detoxified strawberry test-tube seedling rejuvenation medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of rejuvenation medium for virus-free strawberry test-tube plantlets, the formula of which is shown in Table 2. The virus-free strawberry test-tube plantlets infected by bacteria and weak in growth are cut into individual plants, inoculated in rejuvenation medium formula 1, and cultured at a temperature of 22 to 24 ℃, light intensity 1000-200Lux, and daily light time of 10-12 hours under the culture conditions for 30-45 days to complete the first rejuvenation culture; then cut off the old leaves and base of the rejuvenated tissue culture seedlings The root system was transferred to the rejuvenation medium formula 2 for the second rejuvenation culture, and formula 1 and formula 2 were used at intervals. After repeated subculturing for 3 to 4 times, inoculate the original proliferation medium MS+6-BA0.1-0.5mg / L+white sugar 30g / L, the test-tube plantlets recover their growth potential, grow robustly, and have dark green leaves. The proportion of bacterial infection ...

Embodiment 2

[0021] Cut the detoxified strawberry test-tube plantlets infected by bacteria and weak growth potential into individual plants, and inoculate them in the rejuvenation medium formula 2, at a culture temperature of 22-24°C, with a light intensity of 1000-200 Lux, and a daily light time of 10-12 Cultivate for 30 to 45 days under the culture condition of 1 hour to complete the first rejuvenation culture; then cut off the old leaves and basal root system of the tissue cultured seedlings that have been rejuvenated once, and transfer them to the rejuvenation medium formula 4 for the second rejuvenation For cultivation, use formula 3 and formula 4 at intervals. After repeated subculturing for 3 to 4 times, inoculate the original proliferation medium MS+6-BA0.1-0.5mg / L+white sugar 30g / L, the test-tube plantlets recover their growth potential, grow robustly, and have dark green leaves. The proportion of bacterial infection can be reduced by more than 60%.

Embodiment 3

[0023] Cut the detoxified strawberry test-tube plantlets infected by bacteria and weak growth potential into individual plants, inoculate them in the rejuvenation medium formula 3, and cultivate them at a culture temperature of 22-24°C, with a light intensity of 1000-200 Lux, and a daily light time of 10-12 Cultivate for 30 to 45 days under the culture condition of 1 hour to complete the first rejuvenation culture; then cut off the old leaves and basal root system of the tissue cultured seedlings that have been rejuvenated once, and transfer them to the rejuvenation medium formula 4 for the second rejuvenation For cultivation, use formula 3 and formula 4 at intervals. After repeated subculturing for 3 to 4 times, inoculate the original proliferation medium MS+6-BA0.1-0.5mg / L+white sugar 30g / L, the test-tube plantlets recover their growth potential, grow robustly, and have dark green leaves. The proportion of bacterial infection can be reduced by more than 60%.

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Abstract

The invention relates to a cultivation medium for test-tube seedling of detoxified strawberry. The cultivation medium is prepared by mixing a nutrient solution comprising macroelements, iron salts, trace elements and hormone with another material. The macroelements comprise 1100-1237.5 mg / L of ammonium nitrate, 1266.7-1425 mg / L of potassium nitrate, 113.3-127.5 mg / L of potassium dihydrogen phosphate, 246.7-277.5 mg / L of magnesium sulfate heptahydrate and 221.5-249.2 mg / L of calcium chloride. The iron salts comprise 27.8 mg / L of ferrous sulfate heptahydrate and 37.3 mg / L of disodium dihydrogen ethylenediaminetetraacetate. The above-mentioned compounds are mixed to prepare the nutrient solution. The material is vermiculite. The nutrient solution and the vermiculite are mixed according to a volume ratio of 1:1. A difficult problem of test-tube seedling degeneration caused by plant endogenous hormone disorder due to hormone accumulation in repeated subculturing processes of the test-tube seedling of the detoxified strawberry, and difficult problem of bacterial infection in repeated subculturing processes are overcome by the cultivation medium. The production cost is saved. An effective route for production in good time for the test-tube seedling of the detoxified strawberry is provided.

Description

technical field [0001] The invention relates to the technical field of tissue culture test-tube plantlet rejuvenation, in particular to a virus-free strawberry test-tube plantlet rejuvenation medium. Background technique [0002] Strawberry is the plant of the genus Fragaria of the Rosaceae, which belongs to the perennial herb plant, and there are more than 50 known varieties in the world. It is a very popular fruit. Strawberry cultivation began in Europe in the 14th century. The main producing areas are Europe, followed by America and Asia. The average yield per mu is highest in the United States and Canada. [0003] The characteristics of strawberry production in the United States and Canada are: first, virus-free seedlings are used for strawberry planting; my country's main production areas are distributed in Liaoning, Hebei, Shandong, Jiangsu, Shanghai, Zhejiang and other eastern coastal areas. Of course, it is also produced in Xinjiang and other places. The main deto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈权肖建梅张承妹
Owner SHANGHAI SUNQIAO MODERN GREENHOUSE SEED & SEEDLING