Preparation method and detection method of injection for treating lumbar disc herniation
A technique for lumbar intervertebral disc herniation and injection, which is applied in the field of traditional Chinese medicine and can solve the problems of narrow application surface, simple and rough extraction process and detection method, long treatment time and the like.
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Embodiment 1
[0108] Example 1: Take 18 parts of Achyranthes bidentata, 8 parts of Eucommia ulmoides, 25 parts of Sangjisheng and 18 parts of Gentiana chinensis, add 60% to 90% ethanol solution, the amount of ethanol solution is 6 to 8 times, extract by percolation, filter, and combine Alcohol liquid, recover ethanol under reduced pressure and condense into extract with relative density of 1.1-1.5; add 2-3 times of water, heat to 40-60°C and stir to dissolve, add organic solvent for extraction, recover solvent, concentrate and dry to obtain Achyranthes bidentata extract Eucommia ulmoides extract, Sangji extract and Gentiana chinensis extract; take 12 parts of Dilong, add water to decoct twice, each time add 6 to 9 times the amount of water, decoct for 2 to 3.5 hours, combine the two decoctions to concentrate into 1.1-1.5 extract; add 90%-95% ethanol to the extract, stir, precipitate to recover ethanol and concentrate, dry bath at 45-50°C, pulverize into powder to obtain Dilong extract.
Embodiment 2
[0109] Example 2: Take 25 parts of Achyranthes bidentata, 10 parts of Eucommia ulmoides, 35 parts of Sangjisheng and 25 parts of Gentiana chinensis, add water and decoct 2 to 5 times, each time for 1 to 4 hours, combine the decoction, filter, and concentrate the filtrate to 60 For clear ointment with a relative density of 1.15-1.20 °C, add an equal volume of n-butanol or sec-butanol for extraction, separate the extract, concentrate the solvent under reduced pressure to a clear ointment with a relative density of 1.15-1.20 at 60 °C, and depressurize Dry to obtain Achyranthes bidentata extract, Eucommia ulmoides extract, Sangji extract and Gentiana quinquefolium extract.
Embodiment 3
[0110] Example 3: Take 10 parts of Achyranthes bidentata, 5 parts of Eucommia ulmoides, 20 parts of Sangjisheng and 10 parts of Gentiana chinensis, add water to decoct 2 to 5 times, each time for 1 to 4 hours, combine the decoctions, filter, and concentrate the filtrate to a relative density Add ethanol to the clear ointment with a temperature of 1.15-1.20 at 60°C for precipitation and purification, filter, collect the filtrate, recover ethanol under reduced pressure to a clear ointment with a relative density of 1.15-1.20 at 60°C, dry under reduced pressure to obtain Achyranthes bidentata extract extract, Eucommia extract, Sangji extract and Gentiana extract.
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