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A high-density fermentation medium for Haemophilus parasuis

A technology of high-density fermentation of Haemophilus suis is applied in the field of preparation of high-density fermentation medium of Haemophilus parasuis, which can solve the problems of difficult diagnosis, strict pH requirements and high nutritional requirements, and achieves the effect of facilitating rapid growth

Active Publication Date: 2015-09-23
SHANDONG BINZHOU BOLAIWEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to isolate and cultivate under normal conditions, especially the use of antibiotics to treat diseased pigs, which brings difficulties to the diagnosis of the disease; it is reported that the true incidence of Haemophilus parasuis may be 10 times the actual diagnosis many
[0004] At present, there are many problems in the culture of Haemophilus parasuis, such as high nutritional requirements, strict pH requirements, low culture density, etc., and the current commonly used medium is TSB medium, but it cannot meet large-scale cultivation. There are deficiencies in technology

Method used

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  • A high-density fermentation medium for Haemophilus parasuis
  • A high-density fermentation medium for Haemophilus parasuis

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Embodiment 1

[0018] A high-density fermentation medium for Haemophilus parasuis, consisting of the following components and proportioning, according to weight / volume, glutamine 2g / L, yeast extract 15g / L, sodium chloride 5 g / L, carbonic acid Calcium 0.01g / L, magnesium sulfate 0.01g / L, tryptone 1g / L, dipotassium hydrogen phosphate 0.5g / L, by volume ratio: 0.001NAD and 10% bovine serum, the balance is water, before sterilization The pH of the medium was 7.0.

Embodiment 2

[0020] A high-density fermentation medium for Haemophilus parasuis, consisting of the following components and proportioning, according to weight / volume, glutamyl 6g / L, yeast extract 35 g / L, sodium chloride 10 g / L, Calcium carbonate 0.05 g / L, magnesium sulfate 0.05 g / L, tryptone 10 g / L, dipotassium hydrogen phosphate 10 g / L, by volume ratio: 0.01 NAD and 20% bovine serum, the balance is water, sterilized The pH of the pre-medium was 7.2.

Embodiment 3

[0022] The medium consists of the following components and proportions, based on weight / volume, glutamine 3-5g / L, yeast extract 20-30 g / L, sodium chloride 6-8 g / L, calcium carbonate 0.01-0.02 g / L, magnesium sulfate 0.01-0.02 g / L, tryptone 2-8g / L, dipotassium hydrogen phosphate 1.3-8.7 g / L, by volume: 0.005-0.008% NAD and 12-18% bovine serum , the balance is water, and the pH of the culture medium before sterilization is 7.0.

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Abstract

The invention relates to the technical field of microorganism culture mediums and more particularly relates to a preparation method of a high-density fermentation culture medium for haemophilus parasuis. The high-density fermentation culture medium is composed of following components according to a weight / volume ratio: 2-6g / L of glutamine, 15-35g / L of yeast extract, 5-10g / L of sodium chloride, 0.01-0.05g / L of calcium carbonate, 0.01-0.05g / L of magnesium sulfate, 1-10g / L of tryptone and 0.5-10g / L of dipotassium phosphate; the high-density fermentation culture medium further comprises following components in a volume ratio: 0.001%-0.01% of NAD (Nicotinamide Adenine Dinucleotide), 10%-20% of bovine serum and residual amount of water; before sterilization, the pH (Potential of Hydrogen) of the culture medium is 7.0-7.2. The preparation method of the high-density fermentation culture medium for the haemophilus parasuis has the beneficial effects that nutritional ingredients needed by various microorganisms are supplemented to the culture medium and the pH in the culture medium is effectively controlled by the calcium carbonate; a carbon source and a small part of nitrogen are provided by the glutamine so that the high-density fermentation culture medium is good for the rapid growth of the haemophilus parasuis.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, in particular to a method for preparing a high-density fermentation culture medium of Haemophilus parasuis. Background technique [0002] Haemophilus parasuis disease is also known as multiple fibrinous serositis and arthritis, also known as Glaser's disease. Caused by Haemophilus parasuis. The bacterium is ubiquitous in the environment and can be found all over the world, even in healthy pig herds. In herds that are not contaminated with Haemophilus parasuis using specific pathogen-free or medicated early weaning techniques, the consequences of initial infection with this bacterium can be quite severe. [0003] And Haemophilus parasuis: It belongs to the Gram-negative short bacillus with variable morphology and has more than 15 serotypes, of which serotypes 5, 4, and 13 are the most common (accounting for more than 70%). The growth of the bacteria strictly requires nicotinami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/21
Inventor 陈申秒徐海军马景霞吴雪莉王楠
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH