Method and kit for detecting tubercle bacillus

Abstract

The invention provides a primer for detecting tubercle bacillus. The invention also provides a method for detecting tubercle bacillus. The method comprises steps of amplifying IS6110 gene of tubercle bacillus in the detected body through a loop-mediated nucleic acid isothermal amplification method so as to obtain a large number of nucleic acid fragments, then detecting amplified nucleic acid fragments through turbidity of running gel and magnesium pyrophosphate or SYBRGreen fluorescence method. The method can exactly and effectively detect whether the detected body has tubercle bacillus. The invention also provides a kit for detecting the tubercle bacillus.

Description

technical field [0001] The invention relates to a primer for detecting Mycobacterium tuberculosis, in particular to a pair of primers for detecting Mycobacterium tuberculosis with a loop-mediated nucleic acid isothermal amplification method. Background technique [0002] Tuberculosis is a disease of worldwide concern. In April 1993, the World Health Organization declared tuberculosis a global emergency disease and called on all countries to work together to prevent and control it. According to the estimates of the World Health Organization (WHO), about one-third of the world's population has been infected with tuberculosis, and there is a new tuberculosis in almost every second in the world, which also causes nearly two million people in the world every year. Died of tuberculosis. In addition, it is estimated that in the next ten years, 300 million people may be infected, 940,000 people will become ill, and 30 million people will die as a result. In addition, according to ...

AI Technical Summary

Problems solved by technology

However, the average person may not necessarily develop tuberculosis after being infected. Only one tenth of the people will develop tuberculosis, which is called primary TB, and the rest are latent tuberculosis infection (LTBI), which may last their entire lives. None of them will get sick, but about 5% of people will get sick due to the change of immunity, which is called active tuberculosis (Reactivation TB). If the "latent tuberculosis infection" can be diagnosed early, it will effectively monitor and reduce tuberculosis infection

[0004] At present, the clinical methods for diagnosing tuberculosis mainly include smear acid-resistant staining microscopy and tuberculosis culture. Although smear microscopy is simple, its sensitivity is low, only 50% to 60%, and it is usually found in sputum samples. , needs at least 10,000 tubercle bacteria to be found under the microscope; using the tuberculosis culture method, although its specificity is very high, it takes six to eight weeks to get a confirmed diagnosis, so these shortcomings limit this method. Timeliness and efficacy of the two methods in diagnosis and treatment

At present, although many PCR methods are used to detect Mycobacterium tuberculosis, their sensitivity is only about 60% to 80%, and the time required is six to seven hours. Therefore, a faster and more accurate diagnostic method is urgently needed clinically. , to provide physicians with early diagnosis and treatment data

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