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Indoor biological assay method of aphides

A biological assay, aphid technology, applied in biological testing, measuring devices, material inspection products, etc., to save space, reduce damage and errors, and prevent escape

Inactive Publication Date: 2014-03-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problems of various bioassay methods currently widely used in the escape of test insects and bioassay air permeability, the purpose of the present invention is to provide a method for indoor bioassay of aphids

Method used

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  • Indoor biological assay method of aphides

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Embodiment 1

[0032] 1. Research object

[0033] The research object is the field population of peach aphid raised in the laboratory for multiple generations.

[0034] 2. Method

[0035] The tested drug imidacloprid was tested for indoor toxicity by using twelve-well cell culture plates (Corning Costar brand).

[0036] 2.1 Preparation and dilution of the mother liquor of the original drug

[0037] Dissolve the original drug of imidacloprid in acetone, store it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C. Before the toxicity test, it was diluted to various concentrations with distilled water containing 0.05% (v / v) TritonX-100 and set aside. The drug concentration was set at 5 to 7 during the toxicity test, and three simple repetitions were set up.

[0038] 2.2 Selection and use of bioassay leaves

[0039] Choose fresh cabbage leaves, wash with water and dry, and punch holes (diameter d=2cm). Immerse the perforated cabbage lea...

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Abstract

The invention provides an indoor biological assay method of aphides. The method comprises the following steps: (1) laying agar at the bottom of each hole of a cell culture plate; (2) immersing leaves with the same sizes into an insecticide to be assayed for a moment, taking out, airing, and flatly laying the leaves in the cell culture plate by enabling the back surfaces of the leaves to be upward; (3) inoculating a plurality of wingless adult aphides into the holes of the cell culture plate, and sealing the top of the cell culture plate inoculated with the aphides with rice paper strips; (4) putting the cell culture plate into a constant-temperature room, inspecting the death rate all the day, and calculating a toxicity test result. According to the method provided by the invention, the operation is easy and convenient, the space is saved, and damage to the aphides and the error, which are caused by the operation, in a biological assay process are reduced; rice paper is used for sealing, so that the aphides are prevented from escaping, the breathability is improved, the accuracy of a biological assay result is effectively improved, the resistance level of an aphid population can be more comprehensively and accurately known, and a basis can be more accurately provided for reasonably utilizing the insecticide and making a resistance management scheme.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a method for indoor bioassay of aphids. Background technique [0002] Aphids are an important pest in agricultural production, among which peach aphid and cotton aphid are the most serious ones. Among them, green peach aphid is one of the most widely distributed and important agricultural and horticultural pests in the world. It can feed on more than 400 kinds of plants in more than 50 families. , can also spread viruses, cause greater indirect damage to plants, and cause huge economic losses to agricultural production. Peach aphid has a wide range of hosts, many annual generations, and strong reproductive ability. For a long time, the control of peach aphid is mainly based on chemical control. Cotton aphid is also one of the most serious agricultural resistant pests in China, and it is also a worldwide agricultural pest, which mainly harms crops by feeding and sprea...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5085
Inventor 高希武刘晓岚郭慧琳汤秋玲常静
Owner CHINA AGRI UNIV
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