Method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants

A technology of meiosis and parthenogenesis, applied in the field of insect genetic development, can solve the problems of high cost, long breeding cycle, shortening breeding cycle, etc., and achieve the effect of improving combining ability and improving effect

Inactive Publication Date: 2014-04-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a practical silkworm variety suitable for my country (Chinese silkworm variety and Chinese silkworm variety) in view of the lack of novel genetic resources in traditional silkworm breeding in my country, resulting in similar traits, lon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for inducing meiotic parthenogenesis of silkworm by using liquid refrigerant, the method is carried out according to the following steps:

[0021] (1) Collection and cleaning of unfertilized eggs: collect the virgin moths of the silkworm practical variety female 29 after eclosion, and after protecting them at room temperature for 8 hours, pick the abdomen of the virgin moths; The unfertilized eggs in the oviduct pass through the mesh and fall into the collection container, and the large tissue impurities adhere to the mesh and are removed; then the unfertilized eggs peeled off into the collection container are rinsed with tap water , after removing small pieces of tissue fragments, spread the unfertilized silkworm eggs on absorbent paper, dry them at room temperature, and set aside;

[0022] (2) Types of liquid refrigerants and pretreatment: Prepare 20% glycerin aqueous solution with glycerin and water according to the v / v ratio, and put it in the freezer to p...

Embodiment 2

[0028]Method of using liquid refrigerant to induce meiotic parthenogenesis in silkworm In this example, step (1) collection and cleaning of unfertilized eggs: use the virgin moth of silkworm practical variety female 29 after eclosion, and protect it at room temperature for 9 hours; Step (2) Types of liquid refrigerants and pretreatment: Prepare 25% glycerol aqueous solution with glycerin and water according to the v / v ratio, put it in a freezer and pre-cool it to -12°C; pre-cool the large-diameter container to - 12°C; Step (3) Cold shock treatment of unfertilized eggs: put the unfertilized eggs into a small nylon mesh bag, put it into a pre-cooled container, and then pour the pre-cooled glycerin aqueous solution until the mesh bag is covered. Place the cold shock treatment under the condition of -11°C in the freezer for 100 minutes; step (4) protection and acceleration of the cold shock silkworm eggs: put the silkworm eggs in an incubator at 16°C and 80% humidity for 96 hours; ...

Embodiment 3

[0031] In this example, step (1) collection and cleaning of unfertilized eggs: adopt the virgin moth of silkworm practical variety Feng 1 after eclosion, and protect it at room temperature for 10 hours; step (2) type and pretreatment of liquid refrigerant: add chlorine Sodium chloride and water are prepared into a 30% sodium chloride aqueous solution according to the w / v ratio, and then put into a freezer for pre-cooling to -13°C; another large-caliber container is pre-cooled to -13°C; step (3) unfertilized eggs Cold shock treatment: put the unfertilized eggs into a small nylon mesh bag, put it into a pre-cooled container, and then pour in a pre-cooled sodium chloride aqueous solution until the mesh bag is covered, and put it in a freezer at -13°C Under cold shock treatment for 75 minutes; step (4) protection and acceleration of cold shock silkworm eggs: put silkworm eggs in an incubator at 17°C and 85% humidity for 96 hours; the induction result of this example is: a total of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants, belonging to the field of insect genetic breeding technology. The method disclosed by the invention comprises the following steps: (1), collecting and cleaning unfertilized eggs; (2), classifying and preparing the liquid refrigerants; (3), performing cold shock on the unfertilized eggs; and (4), protecting the unfertilized eggs subjected to cold shock and hastening the hatching of the silkworm eggs. According to the invention, a silkworm meiosis type parthenogernesis inducing test achieves success by taking the liquid refrigerants as a medium and Chinese silkworms and Chinese-Japanese crossbreeding silkworms as materials, male silkworm individuals in a genotype homozygous type are obtained, and a new approach is provided for obtaining novel homozygous and true hybrid genetic germplasm resources of Chinese silkworms, and further developing research and utilization of gene expression, excellent character formation, heterosis mechanisms and the like.

Description

technical field [0001] The invention relates to the technical field of insect genetic development, in particular to a method for improving artificially induced meiotic parthenogenesis of silkworms. Background technique [0002] At present, silkworm genetic breeding mainly adopts conventional cross breeding and other means, and cross breeding has played a great role in the five replacements of silkworm varieties in my country. The essence of hybrid breeding is to continuously eliminate bad genes and gather good genes as much as possible into the pure lines of the parents. However, in the practice of silkworm breeding, it is found that in the genome of breeding materials, good genes are often closely linked with some recessive bad genes, resulting in a low efficiency of eliminating bad recessive genes in the breeding process, which generally takes 12 to 15 generations. Only by selection can a genetically stable line (variety) be established. In addition, with the in-depth ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/04
Inventor 翁宏飚刘培刚王永强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap