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Method for synchronously extracting, separating and purifying polysaccharides and flavones of cyclocarya paliurus

A technology of simultaneous extraction and green money, which is applied to medical preparations containing active ingredients, pharmaceutical formulations, plant/algae/fungus/moss components, etc. The problems of low purity and low technical content can achieve the effect of high efficiency, high extraction efficiency and simple extraction process.

Inactive Publication Date: 2014-04-02
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods are mainly water or organic solvent (such as ethanol) direct extraction or reflux extraction, the purity of the extract is too low, the technical content is not high, and it only focuses on the single extraction of total polysaccharides or total flavonoids. At the same time, other active ingredients are discarded, so it is difficult to realize the efficient utilization and industrialization of Cycarya paliurus resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Grind the raw material Cyclocarya paliurus leaves to 10-40 meshes, take 100 g of Cyclocarya paliurus leaves, use 50% alcohol as the extraction solvent, add 800-1000 mL 50% alcohol for the first time and soak for 2-3 h, and slightly boil Keep it warm for 1~2 h, then add 400~600 mL of 50% alcohol for the second time and keep warm for 1~2 h in a slightly boiling state, combine the two extracts, filter, and concentrate the extract to 300 mL under reduced pressure. Pass through a 300-400 mesh filter bag to obtain a clear filtrate, and cool to room temperature. Concentrate pretreatment: The concentrate is adjusted to pH 8.5 with 0.5 mol / L ammonia water and then filtered. The filtrate first passes through a high-pressure column equipped with No. AB-8 macroporous resin, and then passes through a high-pressure column of D301 strong-acid cation exchange resin, and collects the unabsorbed liquid after passing through two high-pressure columns. When the amount of sample loaded is ...

Embodiment 2

[0016] The raw material Cyclocarya paliurus leaves were crushed to 10-40 mesh, 100 g of Cyclocarya leaves were taken, and 50% alcohol was used as the extraction solvent, and 8-10 L of 50% alcohol was added for the first time to soak for 2-3 h, and the micro-boiling state Keep it warm for 1~2h, then add 4~6 L of 50% alcohol for the second time and keep it warm for 1~2 h in a slightly boiling state, combine the two extracts, filter, and concentrate the extract to 3~5L under reduced pressure. Pass through a 300-400 mesh filter bag to obtain a clear filtrate, and cool to room temperature. The filtrate first passes through a high-pressure column equipped with No. AB-8 macroporous resin, and then passes through a high-pressure column of D301 strong-acid cation exchange resin, and collects the unabsorbed liquid after passing through two high-pressure columns. When the amount of sample loaded is raw material (weight g): resin (weight g) = 1:1, the sample loading is completed, and the ...

Embodiment 3

[0018] Grind the raw material Cyclocarya paliurus leaves to 10-40 meshes, take 100 g of Cyclocarya paliurus leaves, use 50% alcohol as the extraction solvent, add 800-1000 mL 50% alcohol for the first time and soak for 2-3 h, and slightly boil Keep it warm for 1~2 h, then add 400~600 mL of 50% alcohol for the second time and keep warm for 1~2 h in a slightly boiling state, combine the two extracts, filter, and concentrate the extract to 300 mL under reduced pressure. Pass through a 300-400 mesh filter bag to obtain a clear filtrate, and cool to room temperature. Concentrate pretreatment: The concentrate is adjusted to pH 8.5 with 0.5 mol / L ammonia water and then filtered. The filtrate is passed through a high-pressure column equipped with AB-8 macroporous resin. When the amount of sample loaded is raw material (weight g): resin (weight g) = 1:1, the sample loading is completed, and the flow rate is 2 mL / min. First eluted with 6-10 BV of water, then 4-6 BV of 70% ethanol and ...

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Abstract

The invention relates to a method for synchronously extracting, separating and purifying polysaccharides and flavones of cyclocarya paliurus. The method is characterized by comprising the steps of pretreating cyclocarya paliurus leaves, then extracting with 30% ethanol, regulating, carrying out concentrate pretreatment, adding diluted alcohol to a sample, carrying out combined series separation with AB-8# macroporous resin and D301 strong-acid-type cation exchange resin, concentrating ethanol, eluting with 0.5mol / L ammonia solution, concentrating and carrying out freeze drying, so as to respectively obtain two bioactive substances, namely flavones and polysaccharides with relatively high purity. According to the method, the disadvantages that currently only simple ingredients can be extracted simultaneously or the extracted ingredients are relatively high in impurity and low in purity and the like are overcome, the content of flavones and the content of polysaccharides are high, the effective separation and purification for flavones and polysaccharides can be realized, and target active substances with relatively high purity can be obtained; the method has the characteristics of high extraction ratio, high product purity and high comprehensive utilization ratio for cyclocarya paliurus leaves.

Description

technical field [0001] The invention belongs to the field of natural product extraction and relates to a method for synchronously extracting active substances from leaves of Cyclocarya paliurus. Background technique [0002] Cyanocarya ( Cyclocarya paliurus (Batal.) Iljinskaja), also known as green money plum, money tree, etc., is a plant of the Juglandaceae Cyclocarya genus. It is a unique single-species plant in China and one of the endangered plants under national key protection. Cyclocarya paliurus is a tall, fast-growing tree with odd-numbered pinnate compound leaves. It is widely distributed in mountains, valleys or valleys at an altitude of 420-2500 m in Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, and Guizhou. Limestone mountains. According to the records of "Chinese Traditional Medicine Chronicles", its bark and leaves have the functions of clearing away heat, detoxifying and relieving pain, and can be used to treat stubborn tinea. Modern st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/52A61K127/00
Inventor 谢明勇谢建华聂少平申明月董彩军
Owner NANCHANG UNIV
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