Multi-sample soil respiration determination film

Abstract

The invention discloses a multi-sample soil respiration intensity determination film, relating to the field of multi-sample soil respiration intensity determination and soil microbial activity detection. The multi-sample soil respiration intensity determination film comprises an upper-layer protection film and a carbon dioxide concentration detection patch in the core part and a lower-layer matrix-borne film, wherein the carbon dioxide concentration detection path is dipped with dye, a buffer agent and a humectant, shows different colors under different carbon dioxide concentration conditions and can determine the respiration intensity of 96 soil samples at the same time. The multi-sample soil respiration intensity determination film disclosed by the invention can improve the multi-sample soil respiration intensity determination efficiency, increase the determination speed and realize a function of continuously monitoring the carbon dioxide concentration, and can greatly reduce the cost of multi-sample soil respiration intensity detection.

Description

technical field [0001] The invention relates to a film for measuring respiration intensity, in particular to a film for measuring respiration intensity of multi-sample soil. Background technique [0002] Currently, there are three techniques for measuring soil microbial metabolic functional diversity, namely multiple substrate-induced respiration technique, Biolog technique and MicroResp technique. TM technology. The measurement principles of these three techniques are basically the same, and they all take advantage of the different characteristics of the substrates that can be utilized by each microorganism that makes up the soil microbial community. By adding different types of carbon source substrates, "community-level metabolism “Fingerprint” or “physiological characteristics at the microbial community level” to determine the characteristics of soil microbial diversity. Its key detection techniques are concentrated on the determination of various soil respiration i...

AI Technical Summary

Problems solved by technology

It was successfully developed by the Macaulay Institute in 2003, but it has been developing slowly since then. The system development is still in its infancy and cannot be called a complete measurement system.

MicroResp TM In the application process of the technology, the measuring device from the carbon source substrate to the respiratory intensity needs to be completely prepared by the user from scratch, and the operation is cumbersome, thus limiting its wide use

Since 2013, the average number of research papers published using this technology is less than 10 per year, and there is no uniform standard for carbon substrate systems, making it difficult for different researchers to make horizontal comparisons

In addition, MicroResp TM There is no special data processing software, and it is completely necessary to use other common office software for calculation, data processing and arrangement, so it is difficult to achieve consensus among different researchers

In addition, its detection technology also uses absorbance for detection, and the culture device and the measurement device need to be separated during the measurement, so the cost of experimental equipment is very high, and continuous measurement cannot be achieved

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