A diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases

A technology for pulmonary interstitial lesions and diagnostic kits, which is applied in disease diagnosis, measuring devices, instruments, etc., can solve problems such as difficulty in repeating, delaying treatment timing, expensive detection methods, etc., and achieves the effect of easy operation

Inactive Publication Date: 2016-05-18
陈娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HRCT detection method is too expensive, and it is not easy to repeat, it cannot detect early ILD, and it is not easy to conduct a general survey of connective tissue diseases; lung biopsy is an invasive surgical examination, which is not easy for patients to accept
The method of alveolar bronchial lavage needs to be obtained by means of bronchoscopy, which is not easy for patients to accept; therefore, patients with early asymptomatic pulmonary interstitial lesions often delay the timing of treatment due to missed diagnosis

Method used

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  • A diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases
  • A diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases
  • A diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 135 cases of rheumatoid arthritis (RA) patients were tested in actual cases, and it was confirmed that the peripheral serum matrix metalloproteinase 7 (MMP7), interferon-γ (IFN-γ) and chemotaxis in patients with connective tissue disease pulmonary interstitial lesions Increased levels of factor IP-10. Detecting the levels of matrix metalloproteinase 7 (MMP7), interferon-γ (IFN-γ) and chemokine IP-10 in peripheral serum of patients can diagnose early pulmonary interstitial lesions of connective tissue disease. The specific verification process is as follows:

[0033] A total of 135 patients with rheumatoid arthritis (RA) were recruited from the Department of Rheumatology and Immunology, The First Affiliated Hospital of Xiamen University. All met the diagnostic criteria for RA in 1982 by the American College of Rheumatology. All the enrolled patients underwent high-resolution pulmonary CT (HRCT) and pulmonary function (PFTs) examinations, and statistics of clinical join...

Embodiment 2

[0046] A diagnostic kit for early-stage pulmonary interstitial lesions of connective tissue disease prepared according to the experimental results of the above embodiment 1, comprising a first ELISA detection component for detecting MMP7 in peripheral serum of patients with connective tissue disease, and a second ELISA for detecting IFN-γ Assay kit and third ELISA assay kit to detect IP-10.

[0047] The preparation method of the above-mentioned 96-well detection plate is as follows:

[0048] Coat 200ng / mL antibody on a 96-well plate at 100uL per well, the antibody diluent is PBS, incubate the 96-well plate at 37°C for 2h; incubate for two hours, wash the plate once, add 200uL of blocking solution to each well, Block at 37°C for 2 hours, wash the plate once after blocking, and place the plate in a drying room to dry before use; the components of the blocking solution are: 1XPBS + 0.5% casein + 2% gelatin + 0.1% preservative (proclin-300).

[0049] The detection process of each...

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Abstract

The invention discloses a connective tissue disease-associated early interstitial lung disease diagnostic kit, comprising a detection module for detecting human peripheral blood MMP7, IFN-gamma and IP-10. The commercial kit provided by the invention is sensitive, safe, reliable and easy to operate; the kit is capable of quantificationally measuring the level of specific protein cytokines and chemotactic factors in human serum, and is helpful for early diagnosis of the connective tissue disease-associated interstitial lung disease.

Description

technical field [0001] The invention specifically relates to a diagnostic kit for early-stage lung interstitial lesions of connective tissue diseases. Background technique [0002] Interstitial pulmonary disease (ILD) is an inflammatory disease of the pulmonary interstitium caused by a variety of causes. Dysfunction due to failure. The early clinical manifestations are shortness of breath after exercise. As the disease progresses, it manifests as a series of symptoms such as dyspnea, cardiac insufficiency, and repeated pulmonary infections. It is a group of diseases with high morbidity and poor prognosis, and its median survival time is only 2 -3 years. There are many diseases that lead to pulmonary interstitial lesions, such as idiopathic interstitial pneumonia (IIP). ILD) patients account for about 15% of the outpatient department of the respiratory department, and 50% of the initial pulmonary interstitial lesions are connective tissue disease pulmonary interstitial les...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535G01N33/531
CPCG01N33/6887G01N2800/10G01N2800/12
Inventor 陈娟史永红王小平葛胜祥刘永亮
Owner 陈娟
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