A kit for detecting trace protein in liquid and its operation method

Abstract

The invention discloses a kit for detecting trace protein in liquid and an operating method thereof. The kit comprises liquid A and liquid B, wherein the liquid A and the liquid B are respectively 0.1M PBS buffer solution containing 0.1M sodium chloride, the liquid A contains carboxylation magnetic beads, the liquid B contains carboxylation microballs, the carboxylation magnetic beads and the carboxylation microballs are coupled to two single cloned antibodies which are prepared by antigen determinant according to different proteins through a carbodiimide method. Compared with the traditional optical waveguide biological sensor, the kit can be reused. By adopting the method, trace target protein molecules in the solution can be rapidly detected, the process is simpler, the efficiency is higher, and a sample can be detected with high flux; by connecting the microballs, the refractive index of a resonant cavity in an optical waveguide immune sensor can be remarkably improved, and the detection sensitivity can be improved.

Description

technical field [0001] The invention relates to the field of medical in vitro detection, in particular to a kit for detecting trace protein in liquid and an operation method thereof. Background technique [0002] In the post-genome era, the research on proteins has attracted more and more attention from researchers at home and abroad. Among them, trace protein has become one of the main targets of clinical and laboratory testing, because it often has very important physiological significance. For example, clinically, it is often necessary to evaluate the content of trace protein in urine (<200mg / L) to monitor the occurrence of nephropathy. In the coagulation test, the content of serine protease (<200mg / L) needs to be monitored, so as to determine the patient's thrombus formation and cardiovascular disease status. Therefore, the development of methods for sensitive detection of trace proteins in liquid is urgently needed. [0003] Among the many trace protein detecti...

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Problems solved by technology

[0003] Among the many trace protein detection methods at present, the traditional turbidimetric method is more convenient and easy, but the specificity is poor; in recent years, the combination method of pyrogallol red and metal complex dye, enzyme-linked immunosorbent method, Methods such as the enzyme-linked aptamer method based on magnetic bead separation are relatively high in sensitivity, but these methods often have the disadvantages of complicated operation and high cost, and are not easy to use on a large scale

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