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Method for preparing collagen polypeptide with large yellow croaker leftovers

A technology of collagen polypeptide and leftovers, applied in the biological field, can solve problems such as polluting the environment and wasting nutrients

Active Publication Date: 2016-09-28
浙江东杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is also abundant in fish viscera, and its molecule has a unique triple helix structure. If it is not treated effectively, it will not only pollute the environment, but also waste a lot of nutrients

Method used

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  • Method for preparing collagen polypeptide with large yellow croaker leftovers

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Embodiment 1

[0016] (1) Preparation of fish scraps mixture: the fish skin, fish scales, and fish viscera are processed separately to remove miscellaneous proteins and facilitate the dissolution of collagen. The fish skin is thawed, washed, cut into pieces, soaked in 0.1mol / L NaOH with 20 times its mass for 2h, and washed for later use; fish scales are thawed, washed, and immersed with 20 times its mass in 0.4mol / L HCl for 2h, mass Soak 20 times of 0.1mol / L NaOH for 2h and wash for later use; the fish innards are thawed, washed, and minced for later use; then the separately processed fish scraps are mixed with fish skin, fish scales and viscera in a certain mass fraction ratio Obtain a mixture of fish scraps and mix well for later use.

[0017] (2) Pick the activated Bacillus coagulans from the nutrient agar slant medium, inoculate it in the nutrient broth seed medium, incubate it on a shaker at 40°C for 12 hours, and then inoculate the seed liquid in the nutrient meat at 6% of the inoculum I...

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Abstract

The invention discloses a method for preparing collagen polypeptides by using larimichthys crocea leftovers. The larimichthys crocea leftovers are fermented by condensed bacillus to obtain collagen polypeptide fermented substances, and the molecular weight of a product is 1,000-3,000 daltons; the larimichthys crocea leftovers comprise fish scales, fish skins and viscera mixtures; the preservation number of the condensed bacillus is CCTCC No:M2013193. According to the method, main nutritional substances produced by fermenting the larimichthys crocea leftovers by the condensed bacillus are free calcium and the collagen polypeptides; acidic products and the calcium which are produced in a fermenting process can be converted into soluble calcium; furthermore, extracellaluar proteases can be used for hydrolyzing proteins in the larimichthys crocea leftovers to supply the collagen polypeptides, ammonia acid, phosphorus and the like which assist in calcium absorption.

Description

Technical field [0001] The invention relates to biotechnology, in particular to a method for preparing collagen polypeptide by fermenting fish scraps by using probiotic Bacillus coagulans. Background technique [0002] In recent years, with the adjustment of my country's aquatic industry structure, the development of aquatic processing industry has gradually grown. However, while processing fish products, a large amount of waste will be produced, which accounts for 30-50% of the total quality of processed fish. Restricted by technology and traditional concepts, for a long period of time, fish scraps were not fully utilized, but were thrown away as garbage. In fact, the processing and comprehensive utilization of fish scraps can not only turn waste into treasure but also avoid environmental pollution. It is also an effective way for enterprises to increase efficiency and increase income for fish and farmers. When people process or eat fish, the leftovers take up a considerable p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12R1/07
Inventor 王彦波傅玲琳李刚
Owner 浙江东杰生物科技有限公司