A kind of extraction separation method of prodigiosin
A prodigiosin and separation method technology, applied in the direction of organic chemistry, can solve the problems of many components, high cost, large proportion, etc., and achieve the effect of high separation purity
Inactive Publication Date: 2016-06-08
JIAXING UNIV
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Problems solved by technology
The purpose of separation and purification of secondary metabolites of microorganisms is to obtain high-purity products from the fermentation broth that meet the quality requirements of food and medicine. However, due to the many and complex components in the fermentation broth, the cost of separation and purification is often high, accounting The proportion of the total cost of the product is very large, and the cost of separation and purification accounts for about 40-70% of different products
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[0031] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0032] figure 1 The flow chart of the extraction and separation method of prodigiosin of the present invention is shown, as shown in the figure, the present invention is realized in this way, a kind of extraction and separation method of prodigiosin comprises the following steps:
[0033] S101: Select laboratory-optimized strains, activate them on a slant surface, and insert them into seed medium for fermentation;
[0034] S102: Extracting prodigiosin: taking the fermentation broth, centrifuging, adding an appropriate amount of acidic acetone, ultrasonically extracting, evaporating to dryness wit...
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The invention discloses a method for extracting and separating prodigiosin. The method for extracting and separating prodigiosin comprises: selecting bacteria strains optimized in a laboratory, activating on an inclined plane, inserting into a seed culture medium, and carrying out fermentation and cultivation; extracting prodigiosin Bacteroerythrin: Take the fermentation broth, centrifuge, add appropriate amount of acidic acetone, extract it by ultrasonic, and dry it with a rotary evaporator to obtain the crude acetone extract, deesterify it with petroleum ether; use thin layer chromatography to carry out silica gel column layer Pre-analysis pre-experiment, by changing the ratio to conduct multiple experiments to get the best solvent ratio; carry out column chromatography; carry out secondary column chromatography separation and purification. The present invention first centrifuges, removes the supernatant that contains less prodigiosin, and uses acidic acetone to carry out crude extraction to the thalline, selects commonly used silica gel column chromatography to carry out fine separation, adopts chloroform-ethyl acetate solvent system and The solvent system gradient elution of chloroform-ethyl acetate-methanol can separate and obtain prodigiosin and unknown substances with high separation purity.
Description
technical field [0001] The invention belongs to the technical field of biochemistry, in particular to a method for extracting and separating prodigiosin. Background technique [0002] Prodigiosin is a natural red pigment mainly produced by microorganisms. It was discovered by Amako et al. in 1929 when they were studying the growth of Serratia, and it was Harashima et al. who first isolated prodigiosin in 1960. . With the in-depth study of the properties and biological activities of prodigiosin, researchers found that it has anti-bacteria, anti-malarial, anti-fungal, anti-protozoa, and the most concerned immunosuppressive activity and the ability to cause tumor cell apoptosis Therefore, as a natural pigment, prodigiosin has great application potential and broad market prospects in the fields of medicine, food, printing and dyeing, textiles, feed additives, and environmental governance. Moreover, prodigiosin, as a pigment produced by microorganisms, overcomes many disadvanta...
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IPC IPC(8): C07D207/44C07G99/00
CPCC07D207/44C07G99/00
Inventor 刘晓侠孙诗清王玉洁尤忠毓
Owner JIAXING UNIV