Sporolactobacillus terrae and application thereof

A technology of Geosporum lactobacillus and Bacillus sp., which is applied in the field of D-lactic acid preparation, can solve the problems of low concentration of D-lactic acid production, long fermentation time and the like, and achieves the effects of simplified operation process, low cost and cost saving

Active Publication Date: 2014-04-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These disclosed technologies, compared with L-lactic acid producing strains and methods, D-lactic acid producing bacterial strains and methods have the shortcomings of too long

Method used

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  • Sporolactobacillus terrae and application thereof
  • Sporolactobacillus terrae and application thereof
  • Sporolactobacillus terrae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Production of D-lactic acid by batch fermentation with Lactobacillus geobacillus (HKM-1) using glucose as carbon source and dry corn steep liquor as nitrogen source in Erlenmeyer flasks

[0072] The composition of each culture medium used in the present embodiment is as follows:

[0073] Each liter of the slant medium contains: 30-50 g of glucose, 5-10 g of yeast powder, 2-8 g of peptone, 50 g of calcium carbonate, 15-25 g of agar powder, and the balance is water. The pH of the slant medium was 6.0. It was sterilized at 115° C. for 15 minutes.

[0074] Each liter of the seed medium contains: 40-120 g of glucose, 5-10 g of yeast powder, 2-8 g of peptone, 50 g of calcium carbonate, and the balance is water. The pH of the seed medium is 5.0-7.0. It is sterilized at 115° C. for 15 minutes.

[0075] Each liter of the fermentation medium contains: 60-120g of glucose, 20g of corn steep liquor dry powder, 50g of calcium carbonate, and water as the balance; the pH of the ferme...

Embodiment 2

[0085] Lactobacillus geobacillus (HKM-1) was used to produce D-lactic acid by batch fermentation in a conical flask with sucrose as carbon source and dry corn steep liquor as nitrogen source:

[0086] The slant medium and seed medium are the same as in Example 1.

[0087] Each liter of the fermentation medium contains: 60-120g of sucrose, 20g of corn steep liquor dry powder, 50g of calcium carbonate, and water as the balance; the pH of the fermentation medium is 5.0-7.0. It is sterilized at 115°C for 15 minutes.

[0088] The method for producing D-lactic acid by fermentation comprises the following steps:

[0089] (1) Incline cultivation: same as Example 1;

[0090] (2) Seed culture: the same as in Example 1;

[0091] (3) Fermentation culture: Put 5mL of the seed culture solution prepared in step (2) into a 100mL Erlenmeyer flask filled with 40mL of fermentation medium, and culture it statically at 42°C. When the rate of change of D-lactic acid is 0, it is regarded as The f...

Embodiment 3

[0097] Lactobacillus geobacillus (HKM-1) was used to produce D-lactic acid by static fermentation in a Erlenmeyer flask with maltose as carbon source and dry corn steep liquor as nitrogen source at 42°C. Fermentation was terminated when the glucose and D-lactic acid levels remained stable.

[0098] The composition of each culture medium used in the present embodiment is as follows:

[0099] The slant medium and seed medium are the same as in Example 1.

[0100] Each liter of the fermentation medium contains: 60-120g of maltose, 20g of corn steep liquor dry powder, 100g of calcium carbonate, and water as the balance; the pH of the fermentation medium is 5.0-7.0, and it is sterilized at 115°C for 15 minutes.

[0101] The method for producing D-lactic acid by fermentation comprises the following steps:

[0102] (1) Incline cultivation: Same as Example 1.

[0103] (2) Seed culture: same as in Example 1.

[0104] (3) Fermentation culture: Put 5mL of the seed culture solution pr...

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Abstract

The invention discloses sporolactobacillus terrae HKM-1 and a method for producing D-lactic acid therefrom through fermentation. The sporolactobacillus terrae HKM-1 is collected in China Center for Type Culture Collection with the collection number CCTCC NO: M2013389, and also collected in DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) with the collection number DSM No.27555. HKM-1 can be used for producing D-lactic acid by using a cheap nitrogen source, namely dry powder of corn steep liquor; the highest fermentation yield of D-lactic acid is 197g/L, the corresponding fermentation period is only 47g, and the fermentation production capacity reaches up to 4.19g/L/h which is far higher than the value publically reported already at present; the strain HKM-1 is stable in high yield performance and capable of producing high-optical purity D-lactic acid, and the optical purity of the produced D-lactic acid is 99.9%; raw materials for fermentation are wide in source and low in cost, and the fermentation state is between anaerobic and micro-aerobic; the method provided by the invention can be used for producing the D-lactic acid through fermentation, and is capable of saving cost and improving production efficiency and also has important industrial application value.

Description

technical field [0001] The invention discloses a bacterial strain in the technical field of D-lactic acid preparation and its application method, specifically a kind of Lactobacillus terreus sporogenes using cheap nitrogen source-corn steep liquor dry powder as an organic nitrogen source to prepare D-lactic acid with high optical purity and high concentration and its application method. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, can be divided into D-lactic acid, L-lactic acid and DL-lactic acid due to different optical activity. Its production methods mainly include chemical synthesis and microbial fermentation. The chemical method can only synthesize DL-lactic acid, while the fermentation method can synthesize a single L-lactic acid, D-lactic acid or DL-lactic acid according to the different strains used. Currently, about 90% of lactic acid is produced by microbial fermentation. Since the human body only has L-lactate dehydrogenase ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/56C12R1/01
CPCC12P7/56C12N1/205C12R2001/01
Inventor 许平黄开明唐鸿志陶飞
Owner SHANGHAI JIAO TONG UNIV
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