Method for building insufficient spleen water-dampness stopping animal model

Abstract

The invention discloses a method for building an insufficient spleen water-dampness stopping animal model. The method comprises the steps that a Wistar rat is placed in a purification cage and fed with high-fat and low-protein feed, the Wistar rat eats and drinks freely and swims exhaustedly every day, and after 40-45 days of modeling, the insufficient spleen water-dampness stopping animal model is successfully built through the method by detecting general rat conditions such as the digestion-absorption functions (comprising the serum gastrin, the urine D-xylose excretion rate, the gastric emptying rate and the intestinal propulsion rate) and the serological indexes (mainly referring to serum albumin and blood fat), and the body mass index and the urine volume tested through a water load test. The method overcomes the detects that there is no insufficient spleen water-dampness stopping animal model at present. A new method for evaluating traditional Chinese medicines in a standard and scientific mode, and has the advantages of being simple in modeling method, easy to operate and popularize and close to clinic.

Description

Technical field [0001] The invention relates to a method for establishing an animal model of spleen deficiency and dampness syndrome. Background technique [0002] Traditional Chinese medicine believes that the spleen is located in the middle Jiao, which is responsible for the transportation and transformation of blood. The spleen transports food and water, absorbs and transports the fine grains and body fluids to the various organs of the body, thereby maintaining the body's life activities. If the spleen fails to function, it will affect the digestion of food and the absorption of fine grains, resulting in abdominal distension, weight loss, and appetite Lack of vibration, fatigue, less qi and blood decline, pale lips, etc. "Su Wen" says: "All dampness and swelling belong to the spleen." The spleen qi plays an important role in the lifting and dispersing movement of water and liquid to maintain the balance of water and liquid metabolism. It will inevitably lead to stagnation o...

AI Technical Summary

Problems solved by technology

There are only reports that some scholars have used the method of simple external dampness or external dampness plus internal dampness to replicate animal models of spleen deficiency and dampness. The method is to increase the temperature and humidity in the living environment of the animals, create external dampness, adopt a high-fat diet, swim, and drink excessively. Such methods damage the spleen and stomach, create internal dampness, and cause animals to show symptoms such as appetite loss, weight loss, loose stools, and rough hair. This type of model still has the following obvious deficiencies: First, the repeatability and Poor stability, lack of uniform modeling standards, too many uncontrollable factors, poor objectivity and standardization

The formation of spleen deficiency is a long-term and chronic process. The syndrome of spleen deficiency cannot be formed in such a short period of time. Although damp resistance appears, it can only be a short-term state, which is easy to recover naturally, and there is a big gap with the syndrome of traditional Chinese medicine.

Third, traditional Chinese medicine believes that the body has symptoms such as drowsiness, fatigue, and listlessness as dampness, so it is not easy to judge dampness.

The above-mentioned model of spleen deficiency and damp resistance mainly uses general conditions such as lack of appetite, poor appetite, curled up, and weight loss as evaluation indicators. It is not specific and difficult to be objective. easy to judge

Fourth, there is a gap with clinical

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