Method for cutting cellulosic material cloth directly produced by utilizing bacteria

A technology of bacterial cellulose and material cloth, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of disconnected technology transformation, high cost, and low output, so as to reduce costs and realize large-scale production , Realize the effect of resource recycling

Inactive Publication Date: 2014-05-14
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the obstacles of high cost, low output, and disconnected technology transformation, bacterial cellulose has not been able to achieve large-scale industrialization in textile industry material products, especially in cloth material products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method of utilizing bacteria to directly produce cellulose material garment cut pieces of the present invention comprises the following steps:

[0030] (1) Preparation of medium:

[0031] Seed medium: peptone 5g / L, yeast powder 5 g / L, citric acid 1 g / L, glucose 20g / L, Na2HPO4 5 g / L, pH 6.0, sterilized at 121°C for 20min;

[0032] Fermentation medium: glucose 25 g / L, Na2HPO4 3 g / L, (NH4)2SO4 5 g / L, ethanol 10 g / L, acetic acid 1 g / L, p-aminobenzoic acid 0.015 g / L, pH 6.0, 121 Sterilize at ℃ for 20 min;

[0033] Culture method: Take a ring of activated slant seeds and insert them into the seed medium, shake and culture them at 30°C and 160 r / min for 24 h as seeds; inoculate the middle medium with an inoculum volume ratio of 8%. Shake fully to make the bacteria liquid uniform, and culture at a constant temperature of 30°C for 8 days;

[0034] (2) Strain cultivation: insert one or two rings of activated Acetobacter xylinum slant seeds into the seed medium, fully shake...

Embodiment 2

[0041] The method of utilizing bacteria to directly produce cellulose material garment cut pieces of the present invention comprises the following steps:

[0042] (1) Preparation of medium:

[0043] Seed medium: peptone 4 g / L, yeast powder 4 g / L, citric acid 1.5 g / L, glucose 15 g / L, Na2HPO4 4 g / L, pH 6.0, sterilized at 120°C for 15 minutes;

[0044] Fermentation medium: glucose 20 g / L, Na2HPO4 2 g / L, (NH4)2SO4 4 g / L, ethanol 8 g / L, acetic acid 0.5 g / L, p-aminobenzoic acid 0.015 g / L, pH 6.0, extinguished at 120 °C Bacteria 15min;

[0045] Culture method: Take a ring of activated slant seeds and insert them into the seed medium, shake and culture them at 30°C and 160 r / min for 24 h as seeds; inoculate the middle medium with an inoculum volume ratio of 8%. Shake fully to make the bacteria liquid uniform, and culture at a constant temperature of 30°C for 8 days;

[0046] (2) Strain cultivation: insert one or two rings of activated Acetobacter xylinum slant seeds into the seed m...

Embodiment 3

[0053] The method of utilizing bacteria to directly produce cellulose material bedding pieces of the present invention comprises the following steps:

[0054] (1) Preparation of medium:

[0055] Seed medium: peptone 6 g / L, yeast powder 6 g / L, citric acid 1.5 g / L, glucose 25 g / L, Na2HPO4 6 g / L, pH 6.0, sterilized at 125°C for 25 minutes;

[0056] Fermentation medium: glucose 30 g / L, Na2HPO4 5 g / L, (NH4)2SO4 6 g / L, ethanol 12 g / L, acetic acid 1.5 g / L, p-aminobenzoic acid 0.015 g / L, pH 6.0, 125 Sterilize at ℃ for 25 minutes;

[0057] Culture method: Take a ring of activated slant seeds and insert them into the seed medium, shake and culture them at 30°C and 160 r / min for 24 h as seeds; inoculate the middle medium with an inoculum volume ratio of 8%. Shake fully to make the bacteria liquid uniform, and culture at a constant temperature of 30°C for 8 days;

[0058] (2) Strain cultivation: insert one or two rings of activated Acetobacter xylinum slant seeds into the seed medium, ...

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Abstract

The invention discloses a method for cutting a cellulosic material cloth directly produced by utilizing bacteria. According to the method, the output of bacteria cellulose is increased through a method of combining ultra-large oxygen supply area design depending on a three-layer composite sheet, timing and concentration subsection oxygen supply, continuous oxygen supply fermentation automatic control (equipment) and a standing fermentation process, and the cost is reduced. The method disclosed by the invention promotes the ideal growth of bacteria cellulose fibers by designing gas-liquid interface area separation and overcomes the defects of long production flow and failure in industry machining of the bacteria cellulose cloth by virtue of combination with a direct preparation technology for developing the bacteria cellulose cloth with proprietary intellectual property right.

Description

technical field [0001] This patent relates to a method for preparing cloth cut pieces, especially a method for directly producing cellulose material cloth cut pieces by using bacteria. Background technique [0002] Bacterial cellulose exists widely in nature, and it was specially produced during the Qin Dynasty in China. However, it is mainly used in beverages. It is also popular in Europe, Japan, the Soviet Union and other countries and regions. Bacterial cellulose is also used in traditional vinegar production. However, the actual separation and application of bacterial cellulose to industrial production and processing has only a history of more than 100 years. Due to insufficient understanding of its physical properties, its application is limited. In the last ten years, with the in-depth understanding of its biosynthesis mechanism and the improvement of fermentation conditions, the industrial application of bacterial cellulose has been accelerated. [0003] Bacterial ce...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
Inventor 张迎晨吴红艳
Owner ZHONGYUAN ENGINEERING COLLEGE
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