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Preparation of natural polyelectrolyte nanofibers with core-shell structure by parallel electric field-induced phase separation

A core-shell structure, parallel electric field technology, applied in fiber processing, fiber chemical characteristics, electrospinning, etc., to achieve the effect of inhibiting cancer cell growth, good water retention, and large specific surface area

Inactive Publication Date: 2016-02-10
HANGZHOU INST OF ADVANCED MATERIAL BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few reports on the preparation of core-shell structure hyaluronic acid and chitooligosaccharide nanofibers by electrospinning

Method used

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  • Preparation of natural polyelectrolyte nanofibers with core-shell structure by parallel electric field-induced phase separation
  • Preparation of natural polyelectrolyte nanofibers with core-shell structure by parallel electric field-induced phase separation
  • Preparation of natural polyelectrolyte nanofibers with core-shell structure by parallel electric field-induced phase separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Prepare a mixed solvent with a mass ratio of deionized water: formic acid: N,N-dimethylformamide = 50:25:25, and directly dissolve hyaluronic acid (Mw=1000000g / mol) in the mixed solvent , stirring at room temperature until the solution is transparent to make a 1wt% hyaluronic acid solution;

[0025] (2) A certain amount of chitosan oligosaccharide (Mw=3000g / mol -1 ) directly dissolved in the hyaluronic acid solution in step 1, so that the mass ratio of hyaluronic acid and chitooligosaccharide in the solution is 1:1, and stirred at room temperature for 2 hours to prepare a uniform electrospinning solution;

[0026] (3) Place the electrospinning solution in step 2 in a self-made parallel electrospinning device, and set the spinning voltage to 3kV under the condition that the spinning solution does not drip, so that the spinning solution is at this voltage Under the pre-phase separation; after 20 minutes, under the condition of room temperature and humidity of 30%, se...

Embodiment 2

[0029] (1) Prepare a mixed solvent with a mass ratio of deionized water: formic acid: N,N-dimethylformamide = 50:25:25, and directly dissolve hyaluronic acid (Mw=1000000g / mol) in the mixed solvent , stirring at room temperature until the solution is transparent to make a 1wt% hyaluronic acid solution;

[0030] (2) A certain amount of chitosan oligosaccharide (Mw=1000g / mol -1 ) directly dissolved in the hyaluronic acid solution in step 1, so that the mass ratio of hyaluronic acid and chitooligosaccharide in the solution is 1:1, and stirred at room temperature for 2 hours to prepare a uniform electrospinning solution;

[0031](3) Place the electrospinning solution in step 2 in a self-made parallel electrospinning device, and set the spinning voltage to 2kV under the condition that the spinning solution does not drip, so that the spinning solution is at this voltage Under the pre-phase separation; after 10 minutes, under the condition of room temperature and humidity of 30%, set...

Embodiment 3

[0033] (1) Prepare a mixed solvent with a mass ratio of deionized water: formic acid: N,N-dimethylformamide = 50:25:25, and directly dissolve hyaluronic acid (Mw=1000000g / mol) in the mixed solvent , stirring at room temperature until the solution is transparent to make a 1wt% hyaluronic acid solution;

[0034] (2) A certain amount of chitosan oligosaccharide (Mw=5000g / mol -1 ) directly dissolved in the hyaluronic acid solution in step 1, so that the mass ratio of hyaluronic acid and chitooligosaccharide in the solution is 1:1, and stirred at room temperature for 2 hours to prepare a uniform electrospinning solution;

[0035] (3) Place the electrospinning solution in step 2 in a self-made parallel electrospinning device, and set the spinning voltage to 4kV under the condition that the spinning solution does not drip, so that the spinning solution is at this voltage Under the pre-phase separation; after 30 minutes, under the condition of room temperature and humidity of 30%, se...

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Abstract

The invention relates a method for preparing polyelectrolyte nano fibers in core-shell structures by a parallel electric field inductive phase separation method. By taking natural products hyaluronic acid and chitosan oligosaccharide as raw materials, the polyelectrolyte nano fibers in core-shell structures are prepared by adopting a high voltage electrospinning technique by means of preparing soluble polyelectrolyte mixed liquor of the natural products hyaluronic acid and chitosan oligosaccharide. According to the invention, phase separation is carried out on polycationic electrolyte and polyanionic electrolyte induced by a parallel electric field, so that the nano fibers in core-shell structures are successfully prepared. The nano fibers in core-shell structures prepared by the invention has the effects of resisting bacteria and stopping bleeding and inhibiting growth of cancer cells and good water retention characteristic, meaning that the effects of resisting bacteria and stopping bleeding and inhibiting growth of cancer cells of the chitosan oligosaccharide of the shell as well as the good water retention effect of hyaluronic acid of the core layer. The nano fibers have a good application prospect in biological tissue engineering, medicines or genetic vector materials, biomedical materials and the like.

Description

technical field [0001] The invention belongs to the field of preparation of biopolymer nanofiber materials, and relates to a method for preparing natural polyelectrolyte nanofibers with a core-shell structure by a parallel electric field-induced phase separation method. Background technique [0002] Electrospinning technology is a method that uses electrostatic repulsion to pull polymer solutions or melts to prepare fibers with diameters ranging from nanometers to micrometers. During the electrospinning process, the charge is carried by the polymer and moves from the injection needle to the receiving device to form a closed electrical cycle, and the high voltage provides energy to accelerate the flow of the polymer. When there is no external voltage, the polymer fluid is stored in the injection needle due to surface tension and does not flow out. When the electric field is turned on, a large amount of electrostatic charge is generated on the surface of the fluid under the f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/18D01D5/00
CPCD01D5/0092D01D5/003D01D5/34D01F8/18D01F9/00
Inventor 马贵平陈广凯聂俊方大为
Owner HANGZHOU INST OF ADVANCED MATERIAL BEIJING UNIV OF CHEM TECH