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Short hairpin ribonucleic acid (shRNA) for transcription inhibition of hepatoma carcinoma cell proliferation by intervening insulin-like growth factor-I (IGF-I) receptor gene and application thereof

A technology of IGF-I and receptor gene, which is applied in the field of molecular biology and biomedicine, can solve the problems of no effective treatment method and ineffective treatment of sorafenib, so as to improve the treatment effect, greatly develop and apply prospects, and inhibit proliferating effect

Inactive Publication Date: 2014-05-21
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, surgical resection or liver transplantation is still the first choice of treatment, and sorafenib is the only drug that can effectively improve the survival rate of patients, but it is ineffective for sorafenib treatment, and most liver cancer patients have already missed the best time for surgery when they are diagnosed. There is currently no effective treatment, therefore, seeking new and effective methods for the treatment of advanced HCC has become a research frontier

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  • Short hairpin ribonucleic acid (shRNA) for transcription inhibition of hepatoma carcinoma cell proliferation by intervening insulin-like growth factor-I (IGF-I) receptor gene and application thereof
  • Short hairpin ribonucleic acid (shRNA) for transcription inhibition of hepatoma carcinoma cell proliferation by intervening insulin-like growth factor-I (IGF-I) receptor gene and application thereof
  • Short hairpin ribonucleic acid (shRNA) for transcription inhibition of hepatoma carcinoma cell proliferation by intervening insulin-like growth factor-I (IGF-I) receptor gene and application thereof

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Embodiment Construction

[0034] The present invention will be further described in detail below in conjunction with specific embodiments, but the embodiments of the present invention are not limited thereto.

[0035] The following content is the design and verification steps of shRNA that interferes with the transcription of IGF-I receptor gene and inhibits the proliferation of liver cancer cells of the present invention.

[0036] (1) Interference sequence design

[0037] According to Genbank’s IGF-IR Gene ID (3480), 4 shRNAs targeting IGF-IR were designed using Ambion’s shRNA design system software. Shanghai Gima Pharmaceutical Technology Co., Ltd. used Ambion’s specific shRNA design software (design2.0 ) Design and synthesis. Dissolve the four oligonucleotide chains (as shown in Table 1) in TE buffer (pH=8.0) with a concentration of 100μM, and take 5μL of 10×shDNA annealing solution (provided by Shanghai Jima Pharmaceutical Technology Co., Ltd.). 5μL of strand oligonucleotide (Table 1, 100nM), 5μL of ant...

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Abstract

The invention discloses a short hairpin ribonucleic acid (shRNA) for transcription inhibition of hepatoma carcinoma cell proliferation by intervening an insulin-like growth factor-I (IGF-I) receptor gene. The shRNA comprises any one or more of the following three shRNA template sequences, namely IGF-IR-shRNA2, IGF-IR-shRNA3 and IGF-IR-shRNA4. By adopting the shRNA, IGF-IR genetic transcription can be efficiently inhibited, so as to inhibit the hepatoma carcinoma cell proliferation and play a sensibilization role on treatment of a targeted or anti-cancer drug. The shRNA disclosed by the invention can be applied to preparation of an anticancer drug. Therefore, the shRNA has a great application prospect and economic value.

Description

Technical field [0001] The present invention relates to the field of molecular biology and biomedicine, in particular to a shRNA that interferes with IGF-I receptor gene transcription and inhibits the proliferation of liver cancer cells and its application. Background technique [0002] Hepatocellular Carcinoma (HCC) is one of the most common malignant tumors. There are more than 600,000 new liver cancer patients every year, ranking third among tumor-related deaths. The occurrence and development of HCC is a complex process of multiple causes, multiple genes, multiple steps and multiple factors, involving multiple signaling pathways and genes such as oncogene activation, tumor suppressor gene inactivation, and / or resurrection of certain oncogenes in the embryonic stage. Regulation. At present, surgical resection or liver transplantation is still the treatment of choice, and sorafenib is the only drug that can effectively improve the survival rate of patients, but it is not effec...

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P1/16
Inventor 姚登福严晓娣姚敏王理
Owner AFFILIATED HOSPITAL OF NANTONG UNIV