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Method for identifying medicinal material veratrum nigrum

A technology of medicinal materials and hellebore, applied in the field of identification of natural medicinal plants

Active Publication Date: 2014-05-21
王顺才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of IGD carbon NMR coupled fingerprint technology in the identification of Veratrum medicinal materials

Method used

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  • Method for identifying medicinal material veratrum nigrum
  • Method for identifying medicinal material veratrum nigrum
  • Method for identifying medicinal material veratrum nigrum

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0089] ④ Preparation of the test solution

[0090] Accurately weigh 200mg of the characteristic extract of Veratrum medicinal material into a 100mL volumetric flask, add appropriate amount of methanol to dissolve, dilute to the mark after ultrasonic oscillation, and shake well to obtain the test solution of the characteristic extract of Veratrum medicinal material.

[0091] ⑤Measurement of precision

[0092] The test solution was injected three times, the relative standard deviation of the peak area was RSD=1.32%, and the relative standard deviation of the retention time was RSD=0.26%.

[0093] ⑥ Determination of the test product

[0094] Draw each test solution, inject a sample, measure its peak area, and obtain the Veratramine content.

[0095] 2) Veratramine absolute content calculation

[0096] ① Calculate the mass concentration of Veratramine in the test solution by the following formula:

[0097] C X = C...

Embodiment 1

[0201] Example 1: IGD carbon NMR spectrum coupling fingerprint of the characteristic extract of Veratrum medicinal material A commercially available

[0202] (1) Preparation of characteristic extracts

[0203] Take 50g of the commercially available Veratrum medicinal material dried in the shade, crush it and pass through a 10-mesh sieve, and use 6, 6, 6 volume times (300mL, 300mL, 300mL) and 95% volume ratio of ethanol to reflux extract 3 times, each extraction 2 hours, the filtrates were combined and concentrated under reduced pressure, and the solvent was recovered until there was no alcohol smell, and the characteristic extract of Veratrum medicinal material was obtained.

[0204] (2) IGD carbon NMR spectrum test of medicinal material characteristic extracts

[0205] Take the characteristic extract of veratrum, disperse it into 5% HOAC (pH 1.0), filter it, extract it with chloroform, adjust the pH of the acidified layer to about 9 with ammonia water, extract it with chloro...

Embodiment 2

[0217] Example 2: IGD carbon NMR spectrum coupling fingerprint of the characteristic extract of Veratrum medicinal material B on the market

[0218] (1) Preparation of characteristic extracts

[0219] Take 100g of commercially available Veratrum medicinal material B that has been dried in the shade, crush it and pass through a 24-mesh sieve, use 8 or 8 volume times (800mL, 800mL) and 90% volume ratio of ethanol to reflux extract twice, extract for 1 hour each time, and combine the filtrates Concentrate under reduced pressure, recover the solvent until it has no alcohol smell, and then obtain the characteristic extract of veratrum.

[0220] (2) IGD carbon NMR spectrum test of medicinal material characteristic extracts

[0221] Take the characteristic extract of veratrum, disperse it into 5% HOAC (pH 1.0), filter it, extract it with chloroform, adjust the pH of the acidified layer to about 9.2 with ammonia water, extract it with chloroform, and recover the solvent from the chlo...

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Abstract

The invention provides a method for identifying a medicinal material veratrum nigrum. The method comprises the following steps: extracting the medicinal material veratrum nigrum to obtain a characteristic extract of the medicinal material veratrum nigrum with an active component group, performing IGD nuclear magnetic resonance carbon spectrum fingerprint spectrum detection on the characteristic extract, obtaining characteristic peak intensities of a plurality of active components in the characteristic extract according to the fingerprint spectrum, measuring the characteristic peak intensities of corresponding standard reference articles of the active components in the same mode, measuring the absolute content of the standard reference articles in the medicinal material veratrum nigrum by using a quantitative analysis method, and calculating the content of the active components in the medicinal material veratrum nigrum and the content of the active component group by using the ratio of the characteristic peak intensities and the absolute content of the standard reference articles. According to the method, the IGD nuclear magnetic resonance carbon spectrum fingerprint spectrum technique is adopted, the compound in the medicinal material veratrum nigrum and the ratio of the compounds are reflected, and the identification on the varieties and the quality of the medicinal material veratrum nigrum is accomplished.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying medicinal materials of veratrum. Background technique [0002] Veratrum nigrum (Veratrum nigrum), also known as black veratrum, mountain onion, etc., is a perennial herb of the genus Veratrum in the family Liliaceae. Province. Traditional Chinese medicine uses the roots and rhizomes of Tianmu Lilu, Maoye Lilu, Mengzi Lilu, Narrow Leaf Lilu, Dali Lilu, Xing'an Lilu, Heizi Lilu, etc., because of its distribution A wide range of main species for medicinal purposes. Veratrum returns to the stomach, lung and liver meridian, cold in nature, pungent and bitter in taste, and is used to treat diseases such as apoplexy, epilepsy, lymphangitis, malaria, mastitis, bone fractures, bruises, tinea capitis, and scabies. It can also be used to kill maggots and flies [①Chinese Academy of Sciences Flora of China Editorial Committee. F...

Claims

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Application Information

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IPC IPC(8): G01N24/08G01N30/02
Inventor 赵天增张海艳董建军常霞陈飞陈玲范毅郭唯魏悦于立芹景炳年刘雨晴
Owner 王顺才
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