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Determination method for alkaloid content in American lotus scape

A technology of alkaloids and Coptis chinensis, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unseen content determination methods, and achieve the effect of good separation and good repeatability

Inactive Publication Date: 2014-05-21
惠铁军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coptidis scape contains a variety of alkaloids. The literature uses spectrophotometry to determine the total alkaloids and high performance liquid chromatography to determine the content of berberine, but there are no major alkaloids in Coptidis scape except berberine. Assay method

Method used

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Embodiment Construction

[0013] The present invention adopts the following technical solutions.

[0014] (1) Use octadecylsilane bonded silica gel column, use 0.1% triethylamine solution as mobile phase A, acetonitrile as mobile phase B for gradient elution, the time-concentration range of mobile phase B is 0min~15min, 15min~25min, 25min~40min, the detection wavelength is 270nm; the flow rate is 1mL min -1 ; The column temperature is 30°C; the number of theoretical plates is not less than 6000 based on the peak of berberine hydrochloride.

[0015] (2) Weigh an appropriate amount of jatrorrhizine hydrochloride, palmatine hydrochloride, and berberine hydrochloride, and prepare 1.06 mg·mL with hydrochloric acid-methanol respectively -1 , 0.48 mg·mL -1 , 0.552 mg·mL -1 solution as a reference stock solution.

[0016] (3) Pipette the stock solution of the above-mentioned reference substance separately, and prepare 16.96 μg·mL with hydrochloric acid-methanol -1 , 57.6 μg·mL -1 , 264....

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Abstract

The invention especially relates to a determination method for alkaloid content in American lotus scape, which belongs to the technical field of determination methods. The determination method provided by the invention has the advantages of easiness and good repeatability. According to the determination method, gradient?elution is carried out by using an octadecylsilane bonded silica gel column with a 0.1% triethylamine solution as a mobile phase A and acetonitrile as a mobile phase B, the time-concentration variation range of the mobile phase B is 0 to 15 min, 15 to 25 min and 25 to 40 min, detection wavelength is 270 nm, flow velocity is 1 mL / min, and column temperature is 30 DEG C; and the number of theoretical plates is no less than 6000, calculated on the basis of berberine hydrochloride peaks.

Description

technical field [0001] The invention belongs to the technical field of determination methods, in particular to a method for determining the content of alkaloids in Coptis scape. Background technique [0002] Coptidis scape is the dried scape of Coptis chinensis. Removing the scape of Coptis chinensis is conducive to the accumulation of alkaloid components and reduces the consumption of nutrients in plants. In Shizhu, Chongqing alone, 2 million kilograms of flowers are discarded every year. The by-products, of which the content of berberine is 1.16%, and its content is similar to that of three needles, have the functions of laxative and laxative and blood fat-lowering, and have certain development and utilization value. Coptidis scape contains a variety of alkaloids. The literature uses spectrophotometry to determine the total alkaloids and high performance liquid chromatography to determine the content of berberine, but there are no major alkaloids in Coptidis scape except b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 惠铁军虞志平蔡文龙
Owner 惠铁军