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Biomarkers von willebrand factor (VWF) and ADAMTS13 and use thereof in cirrhosis diagnosis reagent

A biomarker and application technology, applied in biological testing, disease diagnosis, biomaterial analysis, etc., can solve the problems that patients cannot undergo FibroScan examination, and achieve safety, non-invasive, easy operation, specificity and sensitivity high effect

Active Publication Date: 2014-05-21
重庆早柒天生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A considerable number of patients cannot be examined by FibroScan

Method used

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  • Biomarkers von willebrand factor (VWF) and ADAMTS13 and use thereof in cirrhosis diagnosis reagent
  • Biomarkers von willebrand factor (VWF) and ADAMTS13 and use thereof in cirrhosis diagnosis reagent
  • Biomarkers von willebrand factor (VWF) and ADAMTS13 and use thereof in cirrhosis diagnosis reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Preparation of gold label kit and its application in the diagnosis of liver cirrhosis.

[0061] 1. Preparation of the kit:

[0062] The antibody used in the preparation process of this kit is the specific antibody of VWF or ADAMTS13. Note, during the preparation of the same kit: if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is an antibody of VWF, then the monoclonal antibody 2 and polyclonal antibody used in the preparation of the nitrocellulose-coated membrane should also use VWF Antibody; if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is the antibody of ADAMTS13, then the monoclonal antibody 2 and polyclonal antibody used in the preparation of the nitrocellulose-coated membrane should also use the antibody of ADAMTS13.

[0063] 1. Preparation of colloidal gold solution: put ultrapure water on a magnetic stirrer and stir and heat to boiling, then quickly add chloroauric acid solution...

Embodiment 2

[0084] Example 2: Sensitivity and specificity studies of VWF and ADAMTS13 as biomarkers of liver cirrhosis.

[0085] To define sensitivity and specificity as described here:

[0086] Sensitivity: The sensitivity stated in this article is the proportion of patients with chronic liver disease who actually have cirrhosis who are correctly judged as true positives; or the proportion of patients who actually enter the decompensated stage of liver cirrhosis as true positives.

[0087] Specificity: The specificity described in this article is the proportion of patients with chronic liver disease who do not actually suffer from cirrhosis who are correctly judged as true negative; or the proportion of patients who are actually in compensated cirrhosis who are correctly judged as true negative.

[0088] VWF and ADAMTS13 liver cirrhosis ratio critical value: a ratio data of VWF and ADAMTS13 content specified in this article, if the ratio of VWF and ADAMTS13 content in the subject’s serum...

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Abstract

The invention relates to the field of use of a biomarker for a disease, and discloses biomarkers von willebrand Factor (VWF) and ADAMTS13 and use thereof in a cirrhosis diagnosis reagent. The VWF disclosed by the invention is formed by protein of which the accession number of an amino acid sequence in a database for the national center of biotechnology information (NCBI) is GI317373549; the ADAMTS13 is formed by protein of which the accession number in the database for the NCBI is GI74749836. A testee is subjected to cirrhosis diagnosis by the biomarkers VWF and ADAMTS13, and the biomarkers have the advantages of being simple and feasible, safe and free of a wound in the diagnosis process, easy to be accepted by a patient, small in effect on diagnostic criteria unification caused by personal subjective factors, high in specificity and sensitivity on diagnosis result, easy to carry out a lot of screening for selection, and the like.

Description

technical field [0001] The invention relates to the field of application of disease biomarkers, in particular to liver cirrhosis biomarkers VWF and ADAMTS13 and their use in liver cirrhosis diagnostic reagents. Background technique [0002] Liver cirrhosis is a chronic progressive liver disease with diffuse liver damage caused by long-term or repeated effects of one or more causes. In my country, most of them are post-hepatitis cirrhosis, and a small part are alcoholic cirrhosis and schistosome cirrhosis. Histopathologically, extensive liver cell necrosis, nodular regeneration of residual liver cells, connective tissue hyperplasia, and fibrous septum formation lead to structural destruction of hepatic lobules and formation of pseudolobules. The liver gradually deforms and hardens and develops into cirrhosis. In the early stage, there are no obvious symptoms due to the strong compensatory function of the liver. In the later stage, liver function damage and portal hypertensio...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573G01N33/558
CPCG01N33/543G01N33/558G01N33/6812G01N33/6893G01N2800/085
Inventor 高平
Owner 重庆早柒天生物科技股份有限公司
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