A method for regulating the diversity of soil nematodes by modifying nano-carbon

A soil nematode and nano-carbon technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no significant changes, long time required for metabolism, etc.

Inactive Publication Date: 2016-01-13
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tong et al. (2007) used PCR-DGGE for the first time to evaluate the C 60 For the influence of the anaerobic microbial colony structure and function in the soil, the study found that the structure and function of the microbial community did not change significantly after the soil anaerobic microorganisms were exposed to the experiment for 180 days; Metabolism under anaerobic conditions takes a long time, so related research still needs a long-term observation process; on the other hand, C 60 The impact on the structure and function of microbial communities cannot be extrapolated to other nanoparticles and other microbial communities, and the toxicity of different nanoparticles to microorganisms is quite different
[0007] Therefore, although nano-carbon has a certain passivation effect on heavy metals, the ecological and environmental risks brought about by its restoration of heavy metals are accompanied by

Method used

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  • A method for regulating the diversity of soil nematodes by modifying nano-carbon
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  • A method for regulating the diversity of soil nematodes by modifying nano-carbon

Examples

Experimental program
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Effect test

Embodiment 1

[0076] A method for regulating the diversity of soil nematodes by modifying nano-carbon:

[0077] (1) Handling of materials:

[0078] The collected soil samples were passed through a 2mm sieve and mixed for later use; soil properties: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03mg·kg -1 , saturated water content 0.58mL·g -1 , the soil sample was passed through a 2mm sieve and mixed for later use;

[0079] For lawn plants, tall fescue ( Festuca arundinacea L . );

[0080] The nano-carbon particle size is 20nm, and the specific surface area is 1.2×10 5 m 2 kg -1 , with a pH value of 7, which were modified before application to obtain KMnO 4 、H 2 SO 4 or HNO 3 Modified nano carbon;

[0081] (2) Experimental method:

[0082] 1) The experiment uses a plastic flowerpot with a height of 25cm and a diameter of 12cm. After washing and drying the gauze, cut it into a round shape with a suitable size to fix the bottom of the flowerpot. E...

Embodiment 2

[0086] A method for regulating the diversity of soil nematodes by modifying nano-carbon:

[0087] (1) Handling of materials:

[0088] The collected soil samples were passed through a 2mm sieve and mixed for later use; soil properties: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03mg·kg -1 , saturated water content 0.58mL·g -1 , the soil sample was passed through a 2mm sieve and mixed for later use;

[0089] For lawn plants, tall fescue ( Festuca arundinacea L . );

[0090] The particle size of nano carbon is 70nm, and the specific surface area is 1.2×10 5 m 2 kg -1 , with a pH value of 7, which were modified before application to obtain KMnO 4 、H 2 SO 4 or HNO 3 Modified nano carbon;

[0091] (2) Experimental method:

[0092] 1) The experiment uses a plastic flowerpot with a height of 25cm and a diameter of 12cm. After washing and drying the gauze, cut it into a round shape with a suitable size to fix the bottom of the flowerpot...

Embodiment 3

[0096] A method for regulating the diversity of soil nematodes by modifying nano-carbon:

[0097] (1) Handling of materials:

[0098] The collected soil samples were passed through a 2mm sieve and mixed for later use; soil properties: pH 7.44, organic matter content 4.68, total nitrogen 0.21%, total phosphorus 22.03mg·kg -1 , saturated water content 0.58mL·g -1 , the soil sample was passed through a 2mm sieve and mixed for later use;

[0099] For lawn plants, tall fescue ( Festuca arundinacea L . );

[0100] The nano-carbon particle size is 50nm, and the specific surface area is 1.2×10 5 m 2 kg -1 , with a pH value of 7, which were modified before application to obtain KMnO 4 、H 2 SO 4 or HNO 3 Modified nano carbon;

[0101] (2) Experimental method:

[0102] 1) The experiment uses a plastic flowerpot with a height of 25cm and a diameter of 12cm. After washing and drying the gauze, cut it into a round shape with a suitable size to fix the bottom of the flowerpot. E...

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Abstract

The invention discloses a regulation and control method for improving diversity of nanocarbon to soil nematodes. The method comprises the steps of adopting plastic flowerpots of which the lengths are 25cm and the diameters are 2cm, cleaning and drying gauzes, fixing each gauze which is cut to a round shape with a proper size at the bottom of each flowerpot, and weighing matrix garden soil of 2kg in each container, wherein modified nanocarbon of which the weight is 1-5% that of the matrix garden soil is added into the garden soil respectively; respectively sowing festuca arundinacea seeds of 4.5g respectively; regularly quantitatively watering in the experiment period every day to meet the plant growth demands, culturing the plants in a laboratory, wherein during the cultivation period, the temperature is 25-34 DEG C, the relative humidity is 31-48%, the lighting condition is natural incident light, the experiment time is 120 days, the nematodes in the soil are separated to identify the type and number of the nematodes as the background data of the nematodes, and the type and number of the nematodes are processed after separation and identification after 4 months.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and relates to urban afforestation, in particular to a method for controlling nematode diversity of lawn soil matrix by modified nano-carbon. Background technique [0002] Carbon black is transformed from volatile components of biological or fossil raw materials during incomplete combustion or pyrolysis at high temperatures, and is a product of a gaseous process. Normally, carbon black is a porous nano material with a diameter of 30-50 nm, a large specific surface area and high active sites. [0003] In the 1980s, the global annual production of carbon black was about 50-270Tg (Tg=10 12 g) or so, about 80% of which is due to combustion. Most of the black carbon directly enters the soil, accumulates gradually, and becomes an important component of soil organic matter, and the other part is blown into the atmosphere by the wind. Due to the inertness of carbon black, its photochem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/00
Inventor 多立安赵树兰贺璐
Owner TIANJIN NORMAL UNIVERSITY
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