Preparation method of medical stone carried anti-aeromonas hydrophila agent for sea cucumbers
A technology of Aeromonas hydrophila and medical stone, applied in application, animal feed, animal feed, etc., can solve the problems of easy hydrolysis, etc., and achieve the effects of long storage time, simple processing process and low preparation cost
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Embodiment 1
[0024] (1) Bacteria enrichment: pick a loop from a single colony of the standard strain of Aeromonas hydrophila, inoculate it into ordinary LB medium, and ferment it, and culture it at 37°C for 12 hours to obtain the fermentation product; Insert into the medium of Aeromonas hydrophila for fermentation, culture at 37°C for 48h, until the final concentration of bacteria suspension is 10 10 cfu / g.
[0025] The preparation method of the Aeromonas hydrophila culture medium is as follows: tryptone 10g, yeast extract 5g, sodium chloride 10g, glucose 5g, no need to adjust pH, sterilize at 121°C for 20min; cool for later use.
[0026] (2) Washing: Mix the fermentation product obtained in step (1) with PBS buffer at a mass ratio of 1:50, stir well for 1 min, and then centrifuge at 4000 rpm for 5 min to collect the bacteria;
[0027] (3) Inactivation treatment: Add PBS buffer solution at a mass ratio of 1:50 to the collected bacteria, then add β-propiolactone at a weight ratio of 1:4000...
Embodiment 2
[0032] (1) Bacteria enrichment: pick a loop from a single colony of the standard strain of Aeromonas hydrophila, inoculate it into ordinary LB medium, and ferment it, and culture it at 37°C for 12 hours to obtain the fermentation product; Insert into the medium of Aeromonas hydrophila for fermentation, culture at 37°C for 48h, until the final concentration of bacteria suspension is 10 10 cfu / g.
[0033] The preparation method of the Aeromonas hydrophila culture medium is as follows: tryptone 10g, yeast extract 5g, sodium chloride 10g, glucose 5g, no need to adjust pH, sterilize at 121°C for 20min; cool for later use.
[0034] (2) Washing: Mix the fermentation product obtained in step (1) with PBS buffer at a mass ratio of 1:50, stir well for 1 min, and then centrifuge at 5000 rpm for 5 min to collect the bacteria;
[0035](3) Inactivation treatment: Add PBS buffer solution at a mass ratio of 1:50 to the collected bacteria, and then add β-propiolactone at a weight ratio of 1:5...
experiment example 3
[0040] (1) Bacteria enrichment: pick a loop from a single colony of the standard strain of Aeromonas hydrophila, inoculate it into ordinary LB medium, and ferment it, and culture it at 37°C for 12 hours to obtain the fermentation product; Insert into Aeromonas hydrophila medium for fermentation, culture at 37°C for 35h, until the final concentration of bacterial suspension is 10 9 cfu / g.
[0041] The preparation method of the Aeromonas hydrophila culture medium is as follows: tryptone 10g, yeast extract 5g, sodium chloride 10g, glucose 5g, no need to adjust pH, sterilize at 121°C for 20min; cool for later use.
[0042] (2) Washing: Mix the fermentation product obtained in step (1) with PBS buffer at a mass ratio of 1:50, stir well for 1 min, and then centrifuge at 4000 rpm for 5 min to collect the bacteria;
[0043] (3) Inactivation treatment: Add PBS buffer solution at a mass ratio of 1:50 to the collected bacteria, then add β-propiolactone at a weight ratio of 1:6000 to the...
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