Plant growth-promoting bacteria and preparation method and application of fungicide

A microbial agent, a technology for promoting plant growth, applied in plant growth regulators, plant growth regulators, methods based on microorganisms, etc., can solve the problem of no cultured Bacillus amyloliquefaciens, no tea strains, and different culture methods And other issues

Active Publication Date: 2014-05-28
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up, there are many strains of Bacillus amyloliquefaciens that can antagonize plant diseases, and their cultivation methods are also differ

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Sweet potato starch wastewater produces bacillus amyloliquefaciens microbial bacterial agent

[0017] Pretreatment of sweet potato starch wastewater: adjust the pH of the wastewater to 7.5 with lime water, and sterilize at 115°C for 20 minutes.

[0018] Preparation of liquid seeds: Inoculate Bacillus amyloliquefaciens preserved on a slant into a triangular flask containing pretreated sweet potato starch wastewater, and vibrate at 32° C. at 150 rpm for 26 hours to obtain liquid seeds.

[0019] Liquid fermentation: inoculate 5% (volume ratio) liquid seeds in a fermentation tank equipped with pretreated sweet potato starch wastewater, and cultivate them in sterile air at 32°C for 20 hours; The number of bacteria reaches 5.5×10 9 CFU / ml.

[0020] Bacteria preparation: take the biochar passed through a 30-mesh sieve as the carrier, add the fermentation broth to the biochar according to the weight ratio of fermentation broth: biochar of 1:2, mix well and evenl...

Embodiment 2

[0021] Embodiment 2: Bacillus amyloliquefaciens microbial agent is applied to the production of organic tea

[0022] In the organic tea garden, choose about 2 acres of experimental land with uniform growth of tea trees. The management of the experimental tea trees is the same as that of other tea trees in the base, but the fertilization is different. The test site is divided into 5 blocks, corresponding to 5 treatments respectively. There is an isolation row of about 2 meters between each treatment. The bacterial agent is diluted with tap water or the fermentation broth is directly used. The 5 treatments are respectively clean water control (CK), starch wastewater control (FCK), high concentration (2.5×10 7 CFU / ml) ditch application (H), medium concentration (1.8×10 7 CFU / ml) ditch application (M), low concentration (1.2×10 7 CFU / ml) furrow application (L), according to the amount of 3 liters of bacterial agent dilution per square meter. For each treatment, the bacterial ag...

Embodiment 3

[0023] Embodiment 3: Bacillus amyloliquefaciens microbial bacterial agent is used to prevent cucumber damping-off

[0024] Cucumber damping-off is a disease caused by fungal infection. Collect the soil in the greenhouse where cucumbers have been planted for many years, mix it evenly, put it into flower pots, set up 2 groups of flower pots, 10 pots in each group, and keep higher humidity and lower light in the greenhouse at 15°C to 20°C strength. The first group of flower pots were directly sown with cucumber seeds, 15 seeds per pot, as a control. Before sowing cucumber seeds, the 2nd group was mixed into the soil with a weight ratio of 0.2% Bacillus amyloliquefaciens microbial agent, and then 15 seeds were sown in each pot. The total number of seedlings was counted after unearthed, and the total number of lodging seedlings was counted after the seedlings grew 1 true leaf and before 2 true leaves. The results showed that the lodging rate of the control seedlings was more tha...

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Abstract

The invention relates to a bacillus amyloliquefaciens which can prevent and cure plant disease and promote plant growth, and a low-cost preparation method of fungicide, belonging to the technical field of microbial pesticides and microbial fertilizers. The fungicide is an efficient plant growth-promoting bacteria-bacillus amyloliquefaciens (Bacillus amyloliquefaciens) A3 bacterial strain separated and screened from aquatic plant rhizosphere soil, and is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6676. In the invention, bacillus amyloliquefaciens is cultured in large scale by using sweet potato starch wastewater as a fermentation culture medium to prepare the fungicide which can prevent and cure plant disease and promote plant growth. The process is low in preparation cost and simple to operate, and can also realize resource utilization of the sweet potato starch wastewater and reduce pollutant discharge. The prepared fungicide, used for preparation of tea leaves and vegetables, can not only improve the yield, but also improve the quality of tea gardens and vegetables, so that the bacillus amyloliquefaciens has wide application prospects.

Description

Technical field: [0001] The invention relates to a strain of bacillus amyloliquefaciens that efficiently antagonizes plant pathogenic bacteria and promotes plant growth, and a low-cost production method and application method thereof, belonging to the technical field of microbial pesticides and microbial fertilizers. Background technique: [0002] Bacillus amyloliquefaciens is named for its potential to produce amylase. Bacillus amyloliquefaciens is widely distributed in nature and is an important biocontrol bacterium. It inhibits plant pathogenic bacteria, fungi, viruses and nematodes by producing active substances such as antibiotics, antibacterial proteins or polypeptides, thereby playing a role in biological control ; At the same time, it is also an important class of plant rhizosphere growth-promoting bacteria, showing the effect of promoting growth on a variety of plants. For example, Idriss et al. reported that the Bacillus amyloliquefaciens FZB45 strain has phytase ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14A01N63/00A01P3/00A01P21/00C05F15/00A01C21/00C12R1/07
CPCY02A40/10
Inventor 白志辉周晨光张茉莉徐圣君陈月月肖润林
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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