A method for identifying anti-tumor lymphocytes
A lymphocyte and identification method technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of failure to achieve overall satisfactory results, time-consuming, and difficulties in cancer treatment
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Embodiment 1
[0025] A 0.5 cm tumor mass and its adjacent normal breast tissue mass were surgically resected from a breast cancer patient (patient code LMC5130). In the biosafety cabinet, the tumor tissue was cut into 1 mm square tumor tissue pieces with a scalpel, and the breast normal tissue was also cut into 1 mm square normal tissue pieces. Put the tumor tissue and breast normal tissue in a 24-well culture plate, place a small piece of tumor tissue or normal tissue in each well, and culture each sample in 4 wells. Normal breast tissues were labeled 5130N-1, 5130N-2, 5130N-3, and 5130N-4; breast tumor tissues were labeled 5130Tu-1, 5130Tu-2, 5130Tu-3, and 5130Tu-4. Add 1 ml RPMI-1640 medium and 1000 IU interleukin-2 to each well. Place the 24-well culture plate at 37°C and 5% CO 2 cultured in an incubator. After two days of culture, 1 ml of RPMI-1640 medium and 200 IU of interleukin-2 were added to each well. Place the 24-well culture plate at 37°C and 5% CO 2 Continue to grow in th...
Embodiment 2
[0028] A 0.5 cm tumor mass and its adjacent normal lung tissue mass were surgically resected from a lung cancer patient (patient code LMC576). In a biosafety cabinet, the tumor tissue was cut into small pieces of 1 mm square tumor tissue with a scalpel, and the normal lung tissue was also cut into small pieces of normal tissue of 1 mm square. Place tumor tissue and normal lung tissue in a 24-well culture plate, place a small piece of tumor tissue or normal tissue in each well, and culture each sample in 4 wells. Normal lung tissues were labeled 576N-1, 576N-2, 576N-3 and 576N-4; lung cancer tumor tissues were labeled 576Tu-1, 576Tu-2, 576Tu-3 and 576Tu-4. Add 1 ml RPMI-1640 medium and 1000 IU interleukin-2 to each well. Place the 24-well culture plate at 37°C and 5% CO 2 cultured in an incubator. After two days of culture, 1 ml of RPMI-1640 medium and 200 IU of interleukin-2 were added to each well. Place the 24-well culture plate at 37°C and 5% CO 2 Continue to grow in t...
Embodiment 3
[0031]A 0.5 cm tumor mass and its adjacent normal renal tissue mass were surgically resected from a kidney cancer patient (patient code LMC584). In a biosafety cabinet, the tumor tissue was cut into small pieces of 1 mm square tumor tissue with a scalpel, and the normal kidney tissue was also cut into small pieces of normal tissue of 1 mm square. Put the tumor tissue and normal kidney tissue in a 24-well culture plate, place a small piece of tumor tissue or normal tissue in each well, and culture each sample in 4 wells. Normal kidney tissues were labeled 584N-1, 584N-2, 584N-3, and 584N-4; kidney cancer tumor tissues were labeled 584Tu-1, 584Tu-2, 584Tu-3, and 584Tu-4. Add 1 ml RPMI-1640 medium and 1000 IU interleukin-2 to each well. Place the 24-well culture plate at 37°C and 5% CO 2 cultured in an incubator. After two days of culture, 1 ml of RPMI-1640 medium and 200 IU of interleukin-2 were added to each well. Place the 24-well culture plate at 37°C and 5% CO 2 Continu...
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