Method for rapidly detecting glucose concentration based on gold nanocluster fluorescent probe
A gold nanocluster, glucose concentration technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of unsuitability for general use, high instrument cost, low stability, etc., to achieve easy preparation and storage, high sensitivity, Effects without toxic side effects
Inactive Publication Date: 2014-06-04
NANJING UNIV OF TECH
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In addition, the instruments used in chromatography and spectrophotometry are expensive and require professional experimenters to operate, which is not suitable for widespread use in developing countries and remote areas
In view of the complexity of the glucose structure, the polarimetric method is suitable as an auxiliary detection method; the biosensor method has the advantages of wide linear detection range, high sensitivity, and low cost, and has a good application prospect, but due to its reproducibility Poor and low stability, less used in the actual detection of glucose
[0004] The instruments used in chromatography and spectrophotometry are expensive and need to be operated by professionals; polarimetry can only be used as an auxiliary means to qualitatively detect the simple structure of glucose; Temperature, humidity, air pressure, etc.) are greatly affected, and the stability is poor
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[0022] The present invention will be further described below with reference to the drawings and specific embodiments.
[0023] The glucose fluorescence detection probe is a fluorescent gold nanocluster functionalized with bovine serum albumin (BSA) as a template.
[0024] (1) Synthesis of bovine serum albumin functionalized gold nanoclusters (BSA-Au NCs) fluorescent probes:
[0025] Prepare bovine serum egg (BSA) into a 50mg / mL solution for later use, and add chloroauric acid (HAuCl 4 ) Prepare a solution of 10mmol / L and store in the dark for later use. Take 10 mL of the prepared bovine serum albumin solution and 10 mL of the chloroauric acid solution, mix them in a darkened condition, and stir vigorously in a water bath at a temperature of 37°C for 2 to 3 minutes. Then 1mL sodium hydroxide solution (1mol / L) was added, and the reaction was kept at a constant temperature in the dark for 12 hours. The color of the solution changed from light yellow to light brown, and finally to dark...
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Abstract
The invention discloses a method for rapidly detecting glucose concentration based on a gold nanocluster fluorescent probe. The glucose concentration is detected by taking gold nanocluster subjected to bovine serum albumin functionalization as a glucose fluorescence detection probe through a resonance light scattering technology. Compared with instruments used in chromatography and spectrophotometry, the instruments in the method are low in cost. The raw materials prepared by fluorescence detection have high stability, and the defect that the detection stability is low in a biosensor method is greatly improved. The fluorescence detection probe has the advantages of high sensitivity, high selectivity and low detection limit; the used reagents are non-toxic and do not have any side effect; the method is simple, rapid and easy to operate, and a large instrument is not needed.
Description
Technical field [0001] The invention relates to the technical field of nanomaterial application, in particular to a method for sensitive detection of glucose concentration in blood samples based on a gold nanocluster fluorescent probe. Background technique [0002] Glucose is an indispensable nutrient for metabolism in organisms, an energy source for living cells and an intermediate product of metabolism, and plays a very important role in food analysis and clinical diagnosis. At present, there are many methods for detecting glucose, each with advantages and disadvantages. [0003] Among all the glucose detection methods, the most widely used is high performance liquid chromatography, and the ion chromatography in this method has developed rapidly in recent years. Analyzing glucose by gas chromatography requires silicidation, and the operation is more complicated. Analyzing by spectrophotometry requires adding color reagent. In addition, the cost of instruments used in chromatog...
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IPC IPC(8): G01N21/64
Inventor 陈萌马鸿飞李玉峰冯大千王伟
Owner NANJING UNIV OF TECH