Transgenic constructs and their application in the preparation of epididymal head gene conditional knockout mouse model

Abstract

The invention relates to a transgenic construct and application of the same in preparation of an epididymis head gene conditional knockout mouse model. Specifically speaking, it is discovered by the inventor that an Lcn5 gene promoter sequence with a length of 1.8 kb can drive expression of an exogenous gene in head (especially middle-and-far-end specific) areas of epididymis of a mouse and an exogenous Cre gene expression vector driven by the promoter is thus constructed. The transgenic vector can drive specific expression of an exogenous Cre gene at the head of the epididymis of an animal and expression of other genes like CreERT2 recombinase. Usage of such a transgenic system for preparation of a transgenic mouse, a high genomic integration rate (greater than 25%) can be obtained, and expression of an exogenous gene in medium-and-far-end areas of epididymis heads can be accurately and highly efficiently driven. The invention further establishes an epididymis head specific area conditional gene knockout or overexpression technology platform.

Description

technical field

[0001] The present invention belongs to the field of genetic engineering. Specifically, the present invention relates to a transgenic construct and its application in the preparation of a conditional gene knockout mouse model of the head of the epididymis, especially the establishment of a conditional gene knockout of a specific region of the head of the epididymis Deletion or overexpression technology platform. Background technique

[0002] Transgenic technology is needed to introduce exogenous genes into animal bodies or in-depth study of gene functions. In particular, to explore the functions of genes in animal bodies or improve animal traits, establish new strains, etc., and to study the effects of genes in the epididymis on epididymis development or sperm maturation. Function requires the use of transgene overexpression or gene knockout techniques. On the one hand, these technologies require transgene targeting vectors; on the other hand, in order to co...

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