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Strawberry seedling tissue culture rapid propagation technology

A technology of tissue culture and seedlings, applied in the field of agricultural bioengineering, can solve the problems of cumbersome detoxification technology, high mutation rate, and shortage of seedlings in strawberries, and achieve the effects of reducing inoculation pollution rate, convenient material collection, and less virus

Inactive Publication Date: 2014-06-18
东港市草莓研究所
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Problems solved by technology

For this reason, after more than ten years of scientific and technological research, we have developed the rapid propagation technology of strawberry stem tip tissue culture, which has solved the current problems of strawberry detoxification technology, such as cumbersome, high cost, high mutation rate, slow reproduction, and shortage of seedlings. Broad prospects and high economic benefits

Method used

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Embodiment Construction

[0021] According to the strawberry seedling tissue culture rapid propagation method of the present invention, the method steps are as follows:

[0022] (1) Material collection and cleaning: Select the tip of the stolon. The collection time is generally from mid-May to October or later every year. A large number of stolons occur between June and September, and it is easy to obtain materials. However, during the high-temperature and high-humidity season at this time, there are many bacteria in the materials, and the sterilization is not complete. The explant inoculation is easy to be polluted and the survival rate is low. Select the stolon tip (the size of the first leaf primordium just unfolded) on the pure mother plant in the protected or open strawberry production field, pick it from 3-4cm below the stolon tip, put it in a leaky basket and wash it with natural water clean. The test proves that: the stolons of red strawberries taken from the greenhouse production field from ...

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Abstract

The present invention relates to a strawberry detoxification tissue culture rapid propagation technology, and belongs to the technical field of agricultural bioengineering. The technology comprises: taking stolon tip of strawberry as explant, and carrying out inducing proliferation, secondary culture, rooting culture, transplanting acclimation and other steps so as to rapidly propagate detoxified tissue culture seedlings. According to the present invention, the formula of the culture medium is adjusted, and the stolon tip growth point is adopted to carry out inducing culture so as to achieve direct inducting budding without the callus growth stage, prevent and control varietal character variation and easily perform inoculation operation; characteristics of scientific sampling time, low inoculation contamination rate, high survival rate, simple, rapid and complete detoxification, rapidness, low cost, strong practicality and the like are provided, wherein the propagation coefficient achieves 5-8 times; and the technology is suitable for industrialized seedling breeding, and can be adopted for commercialized production.

Description

technical field [0001] The invention relates to the technical field of agricultural bioengineering, in particular to a virus-free tissue culture and rapid propagation technology for strawberry seedlings. Background technique [0002] my country is a large agricultural country, and the cultivation of fruit trees is developing rapidly, especially the strawberry production area is increasing year by year. Dandong City is the largest strawberry production base in the country. The overall level of strawberry production technology is in a leading position in China, but the overall unit production efficiency is far lower than that of developed countries, and it belongs to poisonous production like the whole country. Historically, strawberry cultivation has been using vegetative methods such as buried stolons or ramets to propagate seedlings, resulting in a high infection rate of various viral diseases, and the plants have no immunity to viruses. Up to now, there are no specific med...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄日静王春花姜兆彤史功成张敬强刁玉峰孙静纪宏宇杜宇斌朱秀珍谷旭琳佟杰郭文普
Owner 东港市草莓研究所
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