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Biocontrol Bacteria, Separation Method, Bacterial Agent and Application for Controlling Sesame Fusarium Wilt

A technology of sesame fusarium wilt and bio-control bacteria agent, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of discounting the effect of fusarium wilt, endangering human health, and natural environment pollution, etc., to achieve Good promotional value, convenient operation, easy storage and transportation

Active Publication Date: 2015-08-05
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the traditional method mainly relied on chemical pesticides to prevent and control crop wilt diseases. However, with the extensive use of chemical pesticides, it has caused serious pollution to the natural environment and endangered human health.
Moreover, the extensive use of chemical pesticides has also caused the pathogenic bacteria of Fusarium wilt to develop drug resistance, and the effect of controlling Fusarium wilt has been greatly reduced.

Method used

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  • Biocontrol Bacteria, Separation Method, Bacterial Agent and Application for Controlling Sesame Fusarium Wilt
  • Biocontrol Bacteria, Separation Method, Bacterial Agent and Application for Controlling Sesame Fusarium Wilt
  • Biocontrol Bacteria, Separation Method, Bacterial Agent and Application for Controlling Sesame Fusarium Wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the isolation of pathogenic bacteria

[0042] Use a sterile cutting knife to cut the stem into 2cm small pieces at the junction of sesame stem disease, sterilize the surface with 75% alcohol for 30 seconds, disinfect the surface with 3% sodium hypochlorite for 3 minutes, and then rinse it with sterile water for 4 times; then use a sterile cutting knife Cut the small section of the stem longitudinally in half from the middle to expose the vascular tissue; gently forceps the central vascular tissue and put it on the PDA medium containing 50 μg / mL chloramphenicol. After 4 days of constant temperature and moisture cultivation in a 26-28 ° C incubator, the dominant colony PDA was selected and cultured for 4 days, and the single spores were isolated and purified, and stored for future use.

Embodiment 2

[0043] Embodiment 2, the separation of soil microorganism

[0044] Take 1g of the root soil of Viola japonica collected, and dilute it to 10 with sterile water gradient. -5 -10 -3 g / mL, spread the diluted mixture evenly on a 1 / 10 TSA culture plate, and incubate it upside down in a constant temperature incubator at 28°C for 2 days. Bacteria with different shapes were separated and purified by streaking on the surface of TSA medium.

Embodiment 3

[0045] Embodiment 3, biocontrol bacteria screening

[0046] The plate confrontation culture method is adopted for the screening of bio-control bacteria. The Fusarium oxysporum block is placed in the center of the PDK plate, and the test bacteria are received at a distance of 25mm from the block, and placed in a 26-28°C incubator for 4-6 days to observe whether there is a bacteriostatic zone. Generate, and measure the size of the inhibition zone, as figure 1 shown.

[0047]The results of antagonism tests showed that a biocontrol fungus against sesame wilt was found in the root soil of Viola chinensis, named SF4-11.

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Abstract

The invention discloses a bio-control bacterium for preventing and treating sesame wilt disease, a separation method, an inoculant, and an application of the inoculant. The bio-control bacterium is paenibacillus peoriae SF4-11; the preservation organization is China Center for Type Culture Collection (CCTCC); the address of the preservation organization is preservation center of Wuhan University, Luojia Mountain road, Wuchang district, Wuhan city, Hubei province; the preservation date is November 28th, 2014; and the preservation number is CCTCC No.M2012489. The bio-control bacterium is prepared by the following steps: carrying out a microorganism separation treatment and a confront culture treatment on soil of Chinese violet roots, and then carrying out a separation treatment so as to obtain the bio-control bacterium for preventing and treating the sesame wilt disease. The bio-control bacterium can effectively prevent and treat the sesame wilt disease. The inoculant prepared from the bio-control bacterium will not generate pollution to the environment during the application process, and thus is harmless. Moreover, the using amount of other chemical pesticides is reduced, the control cost of sesame diseases is reduced, the growth of sesame is promoted, and thus the inoculant and the bio-control bacterium are worthy to promote.

Description

technical field [0001] The invention relates to a biocontrol bacterium for preventing and treating sesame wilt, and at the same time relates to a method for separating the biocontrol bacterium, the biocontrol bacterium agent and its application, and belongs to the technical field of plant protection. Background technique [0002] Fusarium wilt is an important class of plant soil-borne diseases. The main pathogenic bacteria is Fusarium oxysporum. This disease harms plants from the roots of plants and can cause large-scale production reduction of plants. Among them, sesame is the most seriously endangered crop. [0003] Sesamum (Sesamum indicum), belonging to the family Flaxaceae, is one of the oldest oil crops in the world and an important high-quality oil crop in my country. The annual planting area of ​​sesame in my country is about 12 million mu, and the total output is about 750,000 tons, which plays an important role in the international oil trade. Sesame Fusarium wilt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
Inventor 苗红梅张海洋常淑娴魏利斌段迎辉
Owner HENAN ACAD OF AGRI SCI
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