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Method for extracting hickory nut extract with function of promoting neuronal growth

An extraction method and technology of nerve cells, which can be applied to nervous system diseases, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of insufficient research on the mechanism of neuroprotective effect of hickory nuts, and insufficient excavation.

Inactive Publication Date: 2014-07-02
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are a large number of literature and folk evidence that pecan oil has the effect of nourishing the brain, but it is far from enough to explore its effect, mechanism of action and specific functional factors (material basis) of nourishing the brain
As we all know, hickory nuts are rich in nutrition, and the folks generally believe that they are a good food for nourishing the brain. The effect of pecan on learning ability and memory ability, the mechanism of action of pecan and its extract neuroprotection has rarely been studied

Method used

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  • Method for extracting hickory nut extract with function of promoting neuronal growth
  • Method for extracting hickory nut extract with function of promoting neuronal growth
  • Method for extracting hickory nut extract with function of promoting neuronal growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] Pick mature Lin’an hickory nuts, air-dry them in a ventilated place, and remove the shells to obtain 10g of fresh hickory kernels, then quickly freeze 10g of fresh walnut kernels with 20-50ml of liquid nitrogen for 0.5min-10min, discard the liquid nitrogen, and obtain quick-frozen hickory Hard and brittle hickory kernels; then crush the quick-frozen hickory kernels with a pulverizer, and pass through a 100-mesh sieve to obtain hickory nut powder; use 150-250ml petroleum ether for the hickory nut powder under the temperature condition of 50-80°C in a water bath Heat and reflux for extraction for 1-7 hours to obtain an extract; put the extract in a rotary evaporator until the petroleum ether is completely volatilized to obtain 6 ml of hickory nut extract.

experiment example

[0027] Mix pecan extract and DMSO at a volume ratio of 1:3-8, then shake on a shaker at 120-200rpm for 1-4 hours to fully mix the mixture, and take 2.5μl, 5μl, and 10μl of the mixture to act on For human nerve cells, change the cell medium every 2-3 days, add the same amount of mixture again after changing the medium, and continue to culture for 5-10 days, and finally the corresponding hickory extract and medium mass volume concentration are 0.1 mg / ml, 0.2mg / ml, and 0.4mg / ml three groups of experimental examples, observe and count the synapse length of nerve cells, and through significance analysis, it is found that there is a significant difference between the experimental group and the blank group. Among them, the blank group was 0.1% DMSO, and the positive group was basic fiber growth factor bFGF.

[0028] The human neural cell line SH-SY5Y-EGFP expressing green fluorescent protein EGFP was purchased from Hangzhou New Dragon Biotechnology Co., Ltd.

[0029] Observation dia...

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Abstract

The invention discloses a method for extracting a hickory nut extract with a function of promoting neuronal growth. The method comprises the following steps: picking up ripe hickory nuts; drying in the air, and removing shells to obtain the hickory nut kernels; quickly freezing the hickory nut kernels for 0.5-10 minutes with liquid nitrogen, and removing the liquid nitrogen to obtain the quick-frozen hickory nut kernels; crushing the quick-frozen hickory nut kernels by virtue of a crusher to obtain hickory nut powder; heating and performing reflux extraction on the hickory nut powder with a petroleum ether water bath to obtain an extract; and performing rotary evaporation on the extract by using a rotary evaporator until the petroleum ether is completely evaporated to obtain the hickory nut extract. The extraction process is simple and practical, and the hickory nut extract extracted by adopting the method has the effects of remarkably promoting human nerve cell synaptic growth and protecting nerve cells.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an extraction method of hickory nut extract which can promote the growth of nerve cells. Background technique [0002] According to the data of the fifth national census, my country has entered an aging society in 2000, and the aging process is accelerating. By the end of 2007, the proportion of the population aged 65 and above in the total population in my country had reached 9.35%. According to the forecast of the United Nations, by 2030, this proportion will reach 15.9% in my country; by 2050, it will increase to 23.3%. In absolute terms, in 2010, the number of people aged 65 and over in my country reached 111 million; in 2020 it will reach 180 million, in 2030 it will reach 236 million, and in 2050 it will reach 331 million. China in the 21st century will be an irreversible aging society. This is not only a problem of aging population structure, but als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P25/00A61K131/00
Inventor 钱永常黄坚钦王向军周瑜高飞
Owner ZHEJIANG FORESTRY UNIVERSITY