Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of magnolia liliflora pigment A for stabilizing four-chain unit DNA (deoxyribonucleic acid)

A technology of magnolia and quadruplex, which is applied in the application field of magnolia A in stabilizing quadruplex DNA, and can solve the problems of weak selectivity and the like

Active Publication Date: 2014-07-02
MINZU UNIVERSITY OF CHINA
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The planar aromatic ring structure containing nitrogen makes this kind of ligands have stronger solubility and affinity, and also leads to weaker selectivity for interacting with G-quadruplexes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of magnolia liliflora pigment A for stabilizing four-chain unit DNA (deoxyribonucleic acid)
  • Application of magnolia liliflora pigment A for stabilizing four-chain unit DNA (deoxyribonucleic acid)
  • Application of magnolia liliflora pigment A for stabilizing four-chain unit DNA (deoxyribonucleic acid)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1. Magnolin A of formula I stabilizes telomeric G-quadruplex DNA

[0052] A. This example uses fluorescence resonance energy transfer technology (FRET) to confirm that magnolanin A of formula I can stabilize the G-quadruplex formed by the human telomere HTG21 sequence, and increase the melting temperature of the G-quadruplex by 3 Above ℃ (△Tm max =3.2°C). And it is proved that the stabilizing effect has a certain concentration dependence.

[0053] B. In this example, the FRET method is used to complete the selectivity test of formula I magnolanin A to G-quadruplexes. Adding a far excess of double-stranded DNA to the G-quadruplex system has no effect on the reaction results, proving that the formula Ⅰ Magnolin A has high selectivity for G-quadruplexes. The comparison of the binding effect of magnolanin A of formula I on G-quadruplex and hairpin type double helix DNA proves that magnolanin A of formula I has specificity to G-quadruplex.

[0054] C. This exa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of magnolia liliflora pigment A for stabilizing four-chain unit DNA (deoxyribonucleic acid). The magnolia liliflora pigment A shown in formula I is used for stabilizing the four-chain unit DNA. The magnolia liliflora pigment A shown in formula I can stabilize a G-four-chain unit, and the magnolia liliflora pigment A shown in formula I improves the denaturation temperature of the G-four-chain unit for more than 3 DEG C; the magnolia liliflora pigment A shown in formula I has high selectivity and specificity on the G-four-chain unit. The binding constant K of interaction of the magnolia liliflora pigment A shown in formula I and the G-four-chain unit formed by a human telomere HTG21 sequence is equal to 1.49*106, and the combination ratio n is equal to 2.51. The formula is shown in the specification.

Description

technical field [0001] The present invention relates to a new application of magnolanin A, in particular to the application of magnolanin A of formula I in stabilizing quadruplex DNA. Background technique [0002] G-quadruplex is a special kind of nucleic acid secondary structure, and some guanine (G)-rich regions in the human genome have a tendency to form this special structure. G-quadruplex mainly exists in the terminal G repeat sequence of telomere, and many important promoter regions, including c-myc, c-myb, bcl-2, c-kit, VEGF, insulin gene, β-heme gene Wait. The latest research proves that G-quadruplexes exist in the body. After the promoter sequence region of some genes forms a G-quadruplex structure, the transcription and expression levels of the corresponding genes are greatly affected. Therefore, G-quadruplexes The formation or disassembly of chain bodies may involve some important physiological events and processes in vivo, such as apoptosis, cell proliferation,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 王文蜀周爽武海波闫晓倩冯金朝张文浩高超宇
Owner MINZU UNIVERSITY OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com