Method for separating water in alcoholic beverage for assaying isotope of H and O

Abstract

The invention discloses a method for separating water in an alcoholic beverage for assaying an isotope of H and O. An assaying device is built through an N2 gas bottle, a bubble bottle, an absorption pipe, a condensation bottle, a three-way valve and a liquid nitrogen tank, the water in the alcoholic beverage is effectively separated by using an evaporation-capturing-liquid nitrogen condensation technology, and the isotope ratio of the H and the O is assayed through an elemental analysis-stable isotope mass spectrometer. According to the method, the water in various alcoholic beverages can be extracted, and the isotopes of the H and the O of the water are assayed; a sample is completely evaporated and separated, so that isotope fractionation cannot be generated in the process; a drying agent is made of a material which specifically absorbs the water and does not absorb alcohol, and impurity interference cannot be caused after separation. A low-price glass or stainless steel assembly is adopted in the process and can be recycled, so that the cost is saved. The high-accuracy testing capacity can be provided by offline separation assaying.

Description

technical field [0001] This application relates to the field of food origin traceability and isotope technology, in particular, to a method for separating water in alcoholic beverages for H and O isotope determination Background technique [0002] CO 2 -H 2 O oxygen isotope balance and platinum-catalyzed H 2 -H 2 A method for determining the H and O isotope ratios of water in low-alcohol wine based on the principle of O hydrogen isotope balance. Representative instruments such as Gas-Bentch (Thermoelectric Corporation) and Multiflow (isoprime Corporation). [0003] The principle of isotope water balance measurement is mainly to use CO 2 with H 2 Oxygen in O (H 2 with H 2 H in O) undergoes isotope exchange, and when the isotope balance is reached, the CO 2 (H 2 ) into an isotope ratio mass spectrometer (IRMS) to determine the H and O isotope ratios. Since ethanol (C 2 h 5 O) have and CO 2 close molecular mass, so the ethanol enters the mass spectrometer and affe...

AI Technical Summary

Problems solved by technology

Due to ethanol (C 2 h 5 O) have and CO 2 close molecular mass, so the ethanol enters the mass spectrometer and affects the CO 2 Determination of O isotope leads to deviation in O isotope determination

Except for ethanol, any component with similar molecular weight to the measured gas will affect the measurement results

In addition, due to the high ethanol content in high-alcohol wine, high-concentration ethanol will also cause damage to mass spectrometry

Due to the continuous flow principle of commercial mass spectrometry components, the carrier gas continuously converts CO 2 and H 2 Into the mass spectrometer, the difference in the flow rate and equilibrium temperature of the carrier gas makes the measurement accuracy lower than that of the separate measurement method after separation

Finally, due to the high degree of automation commercial components are very expensive

[0004] In summary, there are following shortcoming in the prior art: 1. can only measure the H and O isotope in the low-alcohol wine, there is no effective method for the water isotope determination in the high-alcohol wine

; 2. High equipment cost; 3. Susceptible to matrix interference

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