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Microecological probiotic preparation containing Lactobacillus casei and preparation method thereof

A technology of Lactobacillus casei and probiotics, applied in the field of feed microorganisms, can solve the problems of large environmental pollution, complicated operation of machinery and equipment, difficult post-processing, etc. Effect

Active Publication Date: 2017-03-29
内蒙古科拓微生态科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment

Method used

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  • Microecological probiotic preparation containing Lactobacillus casei and preparation method thereof
  • Microecological probiotic preparation containing Lactobacillus casei and preparation method thereof
  • Microecological probiotic preparation containing Lactobacillus casei and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.

[0047] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.

[0048] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed solution, the inoculum size is 8% (v / w), the fermentation temperature is controlled at 37°C, the humidity is 50%, and fermentation is carried out for 24h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 5% (v / w), the fermentation temperature was controlled at 33°C, the humidity was 50%, and the mixed bacteria were cultured for 12 hours. After t...

Embodiment 2

[0052] Example 2: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.

[0053] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.

[0054] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed liquid, the inoculum size is 2% (v / w), the fermentation temperature is controlled at 37°C, the humidity is 55%, and the fermentation is 36h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 8% (v / w), the fermentation temperature was controlled at 30°C, the humidity was 45%, and the mixed bacteria were cultured for 12 hours. After the fermentatio...

Embodiment 3

[0058] Example 3: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.

[0059] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.

[0060] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed liquid, the inoculum size is 8% (v / w), the fermentation temperature is controlled at 33°C, the humidity is 50%, and the fermentation is 36h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 5% (v / w), the fermentation temperature was controlled at 33°C, the humidity was 50%, and the mixed bacteria were cultured for 18 hours. After the fermentatio...

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Abstract

The invention belongs to the technical field of feed microorganisms and relates to an application technology which takes soybean pulp and bran as substrates, and carries out solid-state fermentation by using probiotics lactobacillus casei HM-09. The technology can be widely applied to animal probiotics micro-ecology and the like; a probiotics solid preparation product is prepared by the following method comprising the following steps: inoculating probiotics lactobacillus casei HM-09 seed liquor on a solid-state fermentation culture medium, then inoculating bacillus licheniformis BL-09, and drying to prepare solid culture powder containing a probiotics agent after fermentation is ended. The collection number of the bacillus licheniformis BL-09 is CGMCC No.5686. The preparation method is low in production cost and simple to operate, and economic benefits can be increased.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and relates to an application technology of solid-state fermentation with probiotic Lactobacillus casei HM-09 (Lactobacillus casei HM-09) using soybean meal and bran as substrates. This technology can be widely used in animal health benefits Bacterial microecology, etc. Background technique [0002] At present, probiotic products mostly use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, and the rest are fermented in liquid state to directly collect probiotic cells and make probiotic powder, tablets or capsules. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. Therefore this application adopts Lactobacillus casei HM-09 (Lactobacillus casei HM-09) solid-state fermented soybean meal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/245C12R1/10A23K10/12A23K10/30A23K10/37
CPCY02P60/87
Inventor 高鹏飞张善亭包维臣
Owner 内蒙古科拓微生态科技发展有限公司
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