Microecological probiotic preparation containing Lactobacillus casei and preparation method thereof
A technology of Lactobacillus casei and probiotics, applied in the field of feed microorganisms, can solve the problems of large environmental pollution, complicated operation of machinery and equipment, difficult post-processing, etc. Effect
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Embodiment 1
[0046] Example 1: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.
[0047] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.
[0048] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed solution, the inoculum size is 8% (v / w), the fermentation temperature is controlled at 37°C, the humidity is 50%, and fermentation is carried out for 24h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 5% (v / w), the fermentation temperature was controlled at 33°C, the humidity was 50%, and the mixed bacteria were cultured for 12 hours. After t...
Embodiment 2
[0052] Example 2: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.
[0053] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.
[0054] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed liquid, the inoculum size is 2% (v / w), the fermentation temperature is controlled at 37°C, the humidity is 55%, and the fermentation is 36h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 8% (v / w), the fermentation temperature was controlled at 30°C, the humidity was 45%, and the mixed bacteria were cultured for 12 hours. After the fermentatio...
Embodiment 3
[0058] Example 3: A probiotic preparation prepared by solid-state fermentation of Lactobacillus casei HM-09, the preparation method of which includes strain culture and solid-state fermentation culture.
[0059] The seed culture conditions: Bacillus licheniformis BL-09 seed culture conditions, placed on a shaker, the rotation speed is 180r / min, the temperature is 37°C, cultivated for 24h; Lactobacillus casei HM-09 is cultured statically at 37°C in an incubator 24h.
[0060] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is inoculated with Lactobacillus casei HM-09 seed liquid, the inoculum size is 8% (v / w), the fermentation temperature is controlled at 33°C, the humidity is 50%, and the fermentation is 36h; then inoculated with lichen Bacillus BL-09, the inoculum amount was 5% (v / w), the fermentation temperature was controlled at 33°C, the humidity was 50%, and the mixed bacteria were cultured for 18 hours. After the fermentatio...
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