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A kind of SNP typing method and kit

A typing method and single-molecule amplification technology, which is applied in the field of SNP typing methods and kits, can solve the problems of low throughput of SNP typing methods, and achieve the effect of avoiding mutual interference and improving throughput

Active Publication Date: 2018-02-13
SHENGZHEN CHINA GENE TECH COMPANY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide a SNP typing method, which aims to solve the problem of low throughput of SNP typing methods in the prior art

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  • A kind of SNP typing method and kit
  • A kind of SNP typing method and kit
  • A kind of SNP typing method and kit

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no. 1 example

[0079] Aiming at realizing the addressable immobilization of the nucleic acid fragment containing the SNP site to be tested in an indirect manner, the present invention proposes a first embodiment: the nucleic acid fragment containing the SNP site to be tested is first fixed on the microsphere, and then the microsphere It is immobilized on a solid phase carrier, so as to realize the addressable immobilization of the nucleic acid fragment containing the SNP site to be tested.

[0080] Further, said step A includes the following steps:

[0081] A1. Use specific primers to perform non-single molecule amplification on the sample to be tested to obtain a nucleic acid fragment containing the SNP site to be tested;

[0082] A2. Immobilize the nucleic acid fragment containing the SNP site to be tested on the microsphere, and then addressably fix the microsphere on the solid phase carrier.

[0083] It should be noted:

[0084] The sample to be tested is any form of sample containing ...

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Abstract

The invention relates to the technical field of DNA detection, and provides an SNP typing method and a kit. The method comprises the following steps: A, fixing a nucleic acid segment containing a to-be-tested SNP site on a solid-phase carrier in an addressable manner; B, sequencing the nucleic acid segment containing the to-be-tested SNP site fixed on the solid-phase carrier, so as to determine the genetype of the to-be-tested SNP site on the nucleic acid segment, wherein the nucleic acid segment containing the to-be-tested SNP site is the product obtained by non-single molecule amplification. The kit comprises a specific primer pair and a solid-phase carrier, wherein the specific primer pair is used for obtaining the nucleic acid segment containing the to-be-tested SNP site by non-single molecule amplification; the solid-phase carrier is used for fixing the nucleic acid segment containing the to-be-tested SNP site in the addressable manner. By adopting the method and the kit disclosed by the invention, mutual interference between different probes can be avoided, and the flux for detecting the SNP site is greatly improved.

Description

technical field [0001] The invention relates to the technical field of DNA detection, in particular to a SNP typing method and a kit. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) refers to the existence of conversion, transversion, insertion, deletion and other changes at a specific nucleotide position in DNA in the genome. At present, the existing SNP typing detection technologies at home and abroad include the following, high resolution melting curve detection method (high resolution melting, HRM), SNP typing method based on chip technology, allele-specific amplification method (Amplification Refractory MutationSystem Real Time PCR, ARMS-RT PCR), Taqman fluorescent probe method, mass spectrometry and high performance liquid chromatography, etc. [0003] Among them, the SNP typing method based on chip technology is most commonly used because it can detect multiple SNP sites at the same time, and the throughput is relati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/10
CPCC12Q1/6869C12Q2565/518C12Q2563/149C12Q2525/191
Inventor 盛司潼钟茂春
Owner SHENGZHEN CHINA GENE TECH COMPANY