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Preparation method and application of molecular imprinting sensor for detecting artemisinin

A molecular imprinting, artemisinin technology, applied in the direction of material electrochemical variables, can solve the problems of poor regeneration and reversibility, high detection limit, affecting the application of molecular imprinting technology, etc., to achieve improved response, high affinity and selectivity. Effect

Inactive Publication Date: 2014-07-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of nanoporous carbon-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and 8 μL of N,N-dimethylformamide dispersion (0.5 g / L) of nanoporous carbon was added dropwise on the surface of the glassy carbon electrode, and placed in an infrared Under the lamp, after the solvent is evaporated, the nanoporous carbon modified glassy carbon electrode is obtained;

[0021] (2) Preparation of oxidized nanoporous carbon: In the reactor, add 35mL concentrated nitric acid, 1.0g nanoporous carbon, 1.0g sodium nitrate, 3.0g potassium permanganate, ultrasonically disperse for 25min, then add 50mL deionized water, Then stirred and reacted at 75°C for 18 h, cooled to room temperature, filtered, washed repeatedly with deionized water until the filtrate was neutral, and dried in vacuum to obtain ...

Embodiment 2

[0025] (1) Preparation of nanoporous carbon-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and 5 μL of N,N-dimethylformamide dispersion (0.5 g / L) of nanoporous carbon was added dropwise on the surface of the glassy carbon electrode, and placed in the infrared Under the lamp, after the solvent is evaporated, the nanoporous carbon modified glassy carbon electrode is obtained;

[0026] (2) Preparation of oxidized nanoporous carbon: In the reactor, add 18mL concentrated nitric acid, 1.0g nanoporous carbon, 0.5g sodium nitrate, 2.0g potassium permanganate, ultrasonically disperse for 20min, then add 25mL deionized water, Then stirred and reacted at 70°C for 20 h, cooled to room temperature, filtered, washed repeatedly with deionized water until the filtrate was neutral, and dried in vacuum to obtain...

Embodiment 3

[0030] (1) Preparation of nanoporous carbon-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3The surface of the powder was polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and 10 μL of N,N-dimethylformamide dispersion (0.5 g / L) of nanoporous carbon was added dropwise on the surface of the glassy carbon electrode, and placed in an infrared Under the lamp, after the solvent is evaporated, the nanoporous carbon modified glassy carbon electrode is obtained;

[0031] (2) Preparation of oxidized nanoporous carbon: In the reactor, add 22mL concentrated nitric acid, 0.5g nanoporous carbon, 1.0g sodium nitrate, 1.0g potassium permanganate, ultrasonically disperse for 20min, then add 18mL deionized water, Then stirred and reacted at 80°C for 16 h, cooled to room temperature, filtered, washed repeatedly with deionized water until the filtrate was neutral, and dried in vacuum to obtain ...

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Abstract

The invention discloses a preparation method of a molecular imprinting sensor for detecting artemisinin. The preparation method is characterized by comprising the following steps of firstly modifying a glassy carbon electrode by utilizing nanometer porous carbon, combining a sol-gel imprinting technique, nanometer platinum particles, a layer-by-layer self assembly method and an electro-polymerization method, and successfully studying an imprinting electrochemical sensor with specific selectivity on the surface of the glassy carbon electrode modified by the nanometer porous carbon. Therefore, the response of the artemisinin molecular imprinting sensor prepared by the method disclosed by the invention is greatly improved. The imprinting sensor has relatively high compatibility and selectivity to artemisinin. The artemisinin molecular imprinting sensor is connected with an electrochemical workstation to form a special template molecular identification sensor. The artemisinin molecular imprinting sensor prepared by the method disclosed by the invention has the advantages that the cost is low, the sensitivity is high, the specificity is good, the detection is quick, and the reusability is available.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of an artemisinin molecularly imprinted sensor, which is based on the specific recognition of molecular imprinting and is used to detect artemisinin in medicines. Artemisinin technology. Background technique [0002] Artemisinin is a new type of sesquiterpene lactone containing peroxy bridge isolated from Artemisia annua. It has pharmacological activities such as antimalarial, antibacterial, antipyretic, and immune enhancement. It has low toxicity and no side effects. It is recommended by the World Health Organization drug of choice in the treatment of malaria. The detection and purification of active ingredients of artemisinin are very important to the quality of medicines. In terms of extraction and separation of natural medicines with very low active ingredients, superc...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 李慧芝李志英李燕
Owner UNIV OF JINAN
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