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Method for preparing drug-loadable low-molecular-weight water-soluble chitosan nano-particle

A water-soluble chitosan, low-molecular-weight technology, applied in pharmaceutical formulations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problems of dissolution, limited application of chitosan, and high viscosity

Inactive Publication Date: 2014-07-30
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the large molecular weight and high viscosity of commonly used chitosan, it cannot be dissolved under physiological pH conditions, which limits the application of chitosan as a vaccine carrier.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The water-soluble chitosan was dissolved in deionized water to prepare 5 ml of 2mg / ml water-soluble chitosan solution. Under the condition of room temperature and magnetic stirring, 1 ml of bovine serum albumin aqueous solution with a concentration of 0.2 mg / ml and 1 ml of sodium tripolyphosphate aqueous solution with a concentration of 0.2 mg / ml were sequentially added, and the drug-loaded nanoparticles were spontaneously generated under the condition of stirring at room temperature. The average particle size of the obtained drug-loaded nanoparticles was 135.5±10.2 nm.

Embodiment 2

[0018] The water-soluble chitosan was dissolved in deionized water to prepare 5 ml of 2.8mg / ml water-soluble chitosan solution. Under the condition of room temperature and magnetic stirring, 1 ml of bovine serum albumin aqueous solution with a concentration of 0.2 mg / ml and 1 ml of sodium tripolyphosphate aqueous solution with a concentration of 0.2 mg / ml were sequentially added, and the drug-loaded nanoparticles were spontaneously generated under the condition of stirring at room temperature. The average particle diameter of the obtained drug-loaded nanoparticles was 99.7±9.4 nm.

Embodiment 3

[0020] The water-soluble chitosan was dissolved in deionized water to prepare 5 ml of 3.6mg / ml water-soluble chitosan solution. Under the condition of room temperature and magnetic stirring, 1 ml of bovine serum albumin aqueous solution with a concentration of 0.2 mg / ml and 1 ml of sodium tripolyphosphate aqueous solution with a concentration of 0.2 mg / ml were sequentially added, and the drug-loaded nanoparticles were spontaneously generated under the condition of stirring at room temperature. The average particle size of the obtained drug-loaded nanoparticles was 110.2±11.3 nm.

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Abstract

The invention provides a method for preparing a drug-loadable low-molecular-weight water-soluble chitosan nano-particle. The method comprises the following steps: a, dissolving water-soluble chitosan in deionized water to prepare a water-soluble chitosan solution with concentration of 2-10mg / ml; b, sequentially adding a bovine serum albumin aqueous solution with concentration of 0.1-0.3mg / ml and sodium tripolyphosphate aqueous solution with concentration of 0.1-0.3mg / ml, and spontaneously generating a drug-loaded nano-particle under room-temperature stirring condition, wherein the volume ratio of the water-soluble chitosan solution to bovine serum albumin aqueous solution to sodium tripolyphosphate aqueous solution is 1:(0.1-0.3):(0.1-0.3). According to the method, the water-soluble chitosan nano-particle can be obtained under a neutral condition and plays a role in sustainably releasing bovine serum albumin and other medicaments, the process is simple and practical, and the nano-particle is small in average grain diameter.

Description

[0001] technical field [0002] The invention relates to a preparation method of low-molecular-weight water-soluble chitosan nanoparticles that can be loaded with drugs. Background technique [0003] Chitosan is the only natural alkaline polysaccharide in nature, its basic unit is glucosamine, insoluble in water, alkaline solution or common organic solvent, but soluble in hydrochloric acid, nitric acid, formic acid, acetic acid and other dilute acids, chitosan With its excellent properties, such as biocompatibility, degradability, non-toxicity, adsorption, and rich sources, sugar has been widely used in the fields of medicine, food, chemical industry, agriculture, and environmental protection. It was approved by Japan, the United States in 1991 Medical circles and nutritional food research institutions in Europe, Europe and other places have identified it as the sixth major life element after carbohydrates, proteins, fats, vitamins and minerals. [0004] Chitosan has biocom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61K47/36
Inventor 王春尹爱国周天
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH