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Method for cultivating silkworm cordyceps

A cultivation method and technology of Cordyceps, which is applied in the field of cultivation of Cordyceps, can solve problems such as susceptibility to septicemia, achieve normal development, protect the ecological environment, and reduce production costs

Inactive Publication Date: 2014-08-06
高青和润丝绸有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silkworms inoculated in mass production are prone to septicemia, how to take measures to solve this problem from the aspects of strain culture, inoculation and environmental control also needs further research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of cultivation method of silkworm caterpillar fungus: comprise the following steps:

[0020] The 19-3, MS1 and 1-Y bacterial classifications are inoculated on the silkworm bodies (the silkworms are reared to the silkworm bodies on the 2nd day of the 5th instar) after being mixed at a mass ratio of 1:1:1; the concentration of the bacterial classifications is 250mg / mL, the injection volume is 3mL; the infection rate after inoculation is 97%, the hardening rate is 95%, and the weed rate is 78%; then aseptic culture, the weed is produced to obtain silkworm caterpillar fungus.

[0021] The inoculation is needle-puncture inoculation, the sterile culture temperature is 15°C, and the air humidity is 85%; light requirements: 20 days after inoculation is the early stage of cultivation, and there is no requirement for light. Photosensitive for 14 hours.

[0022] The silkworm caterpillar fungus that embodiment 1 prepares is tested:

[0023] Cordycepic acid was 49.7, crude...

Embodiment 2

[0025] A kind of cultivation method of silkworm caterpillar fungus: comprise the following steps:

[0026] The 19-3, MS1 and 1-Y bacterial classifications are inoculated on the silkworm bodies (the silkworms are reared to the silkworm bodies on the 3rd day of the 5th instar) after being mixed in a mass ratio of 1:2:3; the concentration of the bacterial classifications is 500mg / mL, the injection volume is 1 mL; after inoculation, the infection rate is 98%, the hardening rate is 96%, and the weed rate is 80%; then aseptic culture, the weed is produced to obtain silkworm caterpillar fungus.

[0027] The inoculation is injection inoculation, the sterile culture temperature is 23°C, and the air humidity is 75%; light requirements: 20 days after inoculation is the early stage of cultivation, there is no requirement for light, and the 20th to 60th day after inoculation is the middle and late stage, requiring 14 Hour.

[0028] The silkworm caterpillar fungus that embodiment 2 prepar...

Embodiment 3

[0031] A kind of cultivation method of silkworm caterpillar fungus: comprise the following steps:

[0032] Inoculate the 19-3 strain on the silkworm chrysalis; the concentration of the strain is 400mg / mL, and the injection volume is 2mL; the infection rate after inoculation is 96%, the hardening rate is 96%, and the weed rate is 75%; Get silkworm grass.

[0033] The inoculation is injection inoculation, the sterile culture temperature is 23°C, and the air humidity is 75%; light requirements: 20 days after inoculation is the early stage of cultivation, there is no requirement for light, and the 20th to 60th day after inoculation is the middle and late stage, requiring 14 Hour.

[0034] The silkworm fungus that embodiment 3 prepares is tested:

[0035] The content of cordycepic acid was 44.3, that of crude polysaccharide was 62.5, that of cordycepin was 2.66, and that of cordycepin adenosine was 1.82.

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Abstract

The invention belongs to the technical field of cordyceps cultivation and particularly relates to a method for cultivating silkworm cordyceps. The method comprises the following steps that strains are inoculated to silkworm bodies or silkworm chrysalises, then axenic cultivation is conducted, and grown grass is the silkworm cordyceps, wherein the strains are one kind or more kinds of a 19-3 strain, an MSl strain and a 1-Y strain. The silkworm cordyceps obtained through the method grows normally, unit grass yield is high, and compared with wild cordyceps, the chemical components and the nutritional ingredients are basically the same. According to the method, the organic combination of the silkworm breeding industry and the cordyceps cultivation is achieved, the method is mature and perfect, production cost can be lowered greatly, the problems that the cordyceps resources are in great shortage and is high in price are solved, preservation of the ecological environment is facilitated, and the silkworm cordyceps is a healthcare product with the homology of medicine and food.

Description

technical field [0001] The invention belongs to the technical field of cordyceps cultivation, and in particular relates to a cultivation method of silkworm caterpillar fungus. Background technique [0002] Wild Cordyceps sinensis is a precious Chinese herbal medicine, which has the functions of tonifying deficiency, benefiting essence, protecting lung, nourishing kidney, stopping bleeding and resolving phlegm, nourishing and strengthening, and preventing and curing cancer. However, natural resources are scarce and expensive, making it difficult to popularize. The artificial cultivation of silkworm fungus can effectively alleviate the contradiction that wild Cordyceps sinensis is on the verge of depletion and is expensive. The distribution of wild Cordyceps sinensis is narrow, the natural parasitic rate is low, and the resources are very limited. In recent years, the ecological environment of the main producing areas of Cordyceps sinensis has been artificially destroyed, and...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05F1/00
CPCY02A40/20
Inventor 苏凯尹遵伟张永华段立平
Owner 高青和润丝绸有限公司
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