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Acquisition method of surface enhanced Raman two-dimensional correlation spectrum

A technology of surface-enhanced Raman and two-dimensional correlation spectroscopy, applied in Raman scattering, spectrometry/spectrophotometry/monochromator, optical radiation measurement, etc., can solve the problem of increased detection cost, sample system damage, disappearance and other problems, to achieve the effect of ingenious perturbation design, low detection cost and easy reproducibility

Active Publication Date: 2014-08-06
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] However, external perturbation accessories or custom perturbation equipment are usually expensive, increasing the cost of detection
Human-imposed external interference may have the disadvantages of destroying the sample system due to excessive temperature or pH change, which is not easy to control and has poor reproducibility, and because the SERS technology itself is very sensitive, it is easily affected by various factors. Interference Slightly excessive can easily lead to the disappearance of SERS "hot spots", thus reducing or disappearing the enhancement effect

Method used

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  • Acquisition method of surface enhanced Raman two-dimensional correlation spectrum
  • Acquisition method of surface enhanced Raman two-dimensional correlation spectrum
  • Acquisition method of surface enhanced Raman two-dimensional correlation spectrum

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Embodiment 1

[0046] In this embodiment one, the dynamic spectrum of the sample to be tested is obtained by a Raman spectrometer, and the dynamic spectrum is processed by existing spectrum processing software, so as to obtain the surface-enhanced Raman two-dimensional correlation spectrum of the sample to be tested. The sample to be tested in this embodiment 1 is pioglitazone hydrochloride, the thin-layer plate selected is silica gel thin-layer plate, and the surface enhancer selected is silver sol.

[0047] 1. Instruments and samples

[0048] (1) Instrument: BWS415-785H portable Raman spectrometer (Bida Tech, USA), excitation wavelength 785nm;

[0049] (2) Preparation of silver sol: take silver nitrate (AgNO 3 ) 17mg was dissolved in 100ml of secondary deionized water, 1.6ml of 1% trisodium citrate was added, heated in a microwave oven on high heat for 6min, the solution was grayish green, cooled to room temperature, added water to the mark, and set aside.

[0050] (3) Preparation of the...

Embodiment 2

[0062] In this embodiment two, the methanol solution of pioglitazone hydrochloride of 1mol / L is taken as the solution of the sample to be tested, the thin-layer plate selected is a silica gel thin-layer plate, and a BWS415-785H portable Raman spectrometer is used to collect relevant dynamic spectra. The silver sol prepared in Example 1 was used as a surface enhancer.

[0063] Set the integration time of the BWS415-785H portable Raman spectrometer to 5s, and the laser intensity to 100mW.

[0064] With reference to the acquisition method of the surface-enhanced Raman two-dimensional correlation spectrum of batch one sample solution in embodiment one, obtain as follows Figure 7 Synchronous and asynchronous spectra of the surface-enhanced Raman 2D correlation spectrum of pioglitazone hydrochloride shown.

Embodiment 3

[0066] In this embodiment three, the methanol solution of pioglitazone hydrochloride of 1mol / L is taken as the solution of the sample to be tested, the thin-layer plate selected is a silica gel thin-layer plate, and a BWS415-785H portable Raman spectrometer is used to collect relevant dynamic spectra. The silver sol prepared in Example 1 was used as a surface enhancer.

[0067] Set the integration time of the BWS415-785H portable Raman spectrometer to 5s, and the laser intensity to 155mW.

[0068] With reference to the acquisition method of the surface-enhanced Raman two-dimensional correlation spectrum of batch one sample solution in embodiment one, obtain as follows Figure 8 Synchronous and asynchronous spectra of the surface-enhanced Raman 2D correlation spectrum of pioglitazone hydrochloride shown.

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Abstract

The invention relates to an acquisition method of a surface enhanced Raman two-dimensional correlation spectrum. According to the method, a dynamic spectrogram of a sample to be tested is acquired through a Raman spectrometer, and the dynamic spectrogram is processed through spectrogram processing software to obtain the surface enhanced Raman two-dimensional correlation spectrum. The acquisition method of the surface enhanced Raman two-dimensional correlation spectrum is characterized in that the acquisition of the dynamic spectrogram includes the following two steps that first, the sample is prepared, and a site to be tested is marked; second, the laser intensity of the Raman spectrometer is set, the site to be tested is continuously irradiated to be infinitesimally disturbed by the laser of the Raman spectrometer at the laser intensity, a series of one-dimensional surface enhanced Raman spectrums of the site to be tested are acquired at the same time, and the dynamic spectrogram of the sample to be tested is obtained. According to the technical scheme, the site of the sample is continuously irradiated to be infinitesimally disturbed by the laser of the Raman spectrometer, operation is easy to perform and repeat, equipment is simple, and detection cost is low.

Description

technical field [0001] The invention relates to a collection method of surface-enhanced Raman two-dimensional correlation spectrum, which uses the Raman spectrometer's own laser to perturb the sample to be measured, obtains a series of dynamic spectra of the sample to be measured, and then obtains the two-dimensional correlation spectrum of the sample to be measured Synchronous and asynchronous spectra of spectra. Background technique [0002] Surface Enhanced Raman Scatting (SERS) means that when some molecules are adsorbed to the surface of some rough metals (silver, gold, copper, etc.), their Raman scattering will increase by 10% compared with normal Raman scattering 3 ~10 6 times. There are many compounds that can produce the SERS effect, among which the molecules with lone pair electrons or π electron clouds exhibit the strongest SERS effect. [0003] In recent years, with the popularity of portable Raman spectrometers, the use of surface-enhanced Raman spectroscopy ...

Claims

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Application Information

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IPC IPC(8): G01J3/44G01N21/65
Inventor 陆峰朱青霞李皓柴逸峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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