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Method for determining retention capacity of albendazole in mulberry leaves

A technology for albendazole and its determination method, which is applied in the field of determination of albendazole retention in mulberry leaves, can solve the interference of albendazole detection, cannot realize the separation and purification of albendazole, and cannot Determination and other problems, to achieve the effect of simple method, good enrichment and high accuracy

Active Publication Date: 2014-08-06
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the composition difference between plant matrix tissue and animal matrix tissue, the solid phase extraction conditions of the prior art cannot realize the separation and purification of albendazole in plant matrix tissue, especially flavonoids, alkaloids and Components such as phytosterols will seriously interfere with the detection of albendazole, so that the content of albendazole in the mulberry leaf matrix tissue cannot be directly determined by high performance liquid chromatography

Method used

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  • Method for determining retention capacity of albendazole in mulberry leaves
  • Method for determining retention capacity of albendazole in mulberry leaves
  • Method for determining retention capacity of albendazole in mulberry leaves

Examples

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Effect test

Embodiment 1

[0035] A kind of assay method of albendazole retention in mulberry leaf, concrete operation steps are as follows:

[0036] (1) Sample extraction: Accurately weigh 0.8g of dry mulberry leaf powder to be tested, soak in 80g of acetonitrile, extract by vortexing for 20min, vacuum filter the feed solution, transfer the filtrate to a 100mL stoppered measuring cylinder, add 6g of NaCl, and shake After 1 min, let it stand for stratification, absorb 40.0 g of the upper layer acetonitrile solution and concentrate it to 1 / 20 in a rotary evaporator at 45°C, and add 2 g of methanol-dichloromethane mixture with a volume ratio of 10:90 to dissolve to obtain the liquid to be purified;

[0037] (2) Sample purification: first use 5mL of methanol:dichloromethane with a volume ratio of 10:90 methanol-dichloromethane mixture to rinse the amino solid-phase extraction column. The size of the amino solid-phase extraction column is 6mL in volume and 300mg in filler , when the solvent liquid level rea...

Embodiment 2

[0045] A kind of assay method of albendazole retention in mulberry leaf, concrete operation steps are as follows:

[0046] (1) Sample extraction: Accurately weigh 0.6g of dry mulberry leaf powder to be tested, add 60g of acetonitrile to soak, extract by vortexing for 25min, vacuum filter the feed solution, transfer the filtrate to a 100mL stoppered measuring cylinder, add 4g of NaCl, and shake After 1 min, let it stand for stratification, absorb 50.0g of the upper layer acetonitrile solution and concentrate it to 1 / 20 in a rotary evaporator at 45°C, add 3g of methanol-dichloromethane mixture with a volume ratio of 10:90 to dissolve to obtain the solution to be purified;

[0047] (2) Sample purification: first use 5mL of methanol-dichloromethane mixture with a volume ratio of 20:80 to rinse the amino solid-phase extraction column. , when the solvent level reaches the surface of the column adsorption layer, discard the eluent, immediately add the liquid to be purified in step (1...

Embodiment 3

[0055] A kind of assay method of albendazole retention in mulberry leaf, concrete operation steps are as follows:

[0056] (1) Sample extraction: Accurately weigh 1.0g of dry mulberry leaf powder to be tested, add 100g of acetonitrile to soak, extract by vortexing for 10min, vacuum filter the feed solution, transfer the filtrate to a 100mL stoppered measuring cylinder, add 8g of NaCl, and shake After 1 min, let it stand for stratification, absorb 30.0 g of the acetonitrile solution in the upper layer and concentrate it to 1 / 15 in a rotary evaporator at 45 ° C, and add 1 g of methanol-dichloromethane mixture with a volume ratio of 10:90 to dissolve to obtain the liquid to be purified;

[0057] (2) Sample purification: first use 5mL of methanol-dichloromethane mixture with a volume ratio of 5:95 to rinse the amino-SPE column. The amino-SPE column is 5mL in volume and 500mg in filler , when the solvent level reaches the surface of the column adsorption layer, discard the eluent, ...

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Abstract

The invention belongs to the technical field of drug testing and discloses a method for determining the retention capacity of albendazole (ABZ) in mulberry leaves. According to the method, a solid phase extraction method is adopted for purifying a target substance, and purification adopts an amino solid-phase extraction column; a mixed solution of methyl alcohol-dichloromethane with the volume ratio being (5-20):(80-95) is used for activating the solid-phase extraction column, and a mixed solution of methyl alcohol-dichloromethane with the volume ratio being (1-10):(90-99) is used for drip washing, so that interfering components in the mulberry leaves are almost completely adsorbed by packing of the extraction column, and the target component, namely albendazole, is almost completely eluted out, and thus, the albendazole in the mulberry leaf tissue is separated and enriched well, and a purified sample can be directly quantified through liquid chromatogram. The method has the advantages of simplicity, easiness, low cost, good repeatability of detection result and high degree of accuracy.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method for determining the retention of albendazole in mulberry leaves. Background technique [0002] Sericulture in China has a history of more than 5,000 years. The production and sales of silk and silk products in my country account for about 70% of the world's total. It is the only export commodity that can monopolize the world trade market. Silkworm microparticles have the ability to infect embryos and have a devastating impact on silkworm egg production due to their ability to infect germs. They are quarantine objects in silk-producing countries in the world. In the past 20 years, silkworm microparticle disease has become popular in my country, and millions of super-toxic silkworm eggs have been eliminated, resulting in heavy economic losses. Existing studies have shown that albendazole is an ideal drug for the treatment of silkworm microparticle disease (Yang Qio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 杨琼李丽邢东旭肖阳李庆荣叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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